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. 1990 Dec;172(12):6736–6740. doi: 10.1128/jb.172.12.6736-6740.1990

Tn10-derived transposons active in Bacillus subtilis.

M A Petit 1, C Bruand 1, L Jannière 1, S D Ehrlich 1
PMCID: PMC210787  PMID: 2174858

Abstract

Small derivatives of the Escherichia coli transposon Tn10, comprising IS10 ends and a chloramphenicol resistance gene, were introduced in Bacillus subtilis on a thermosensitive plasmid, pE194. In the presence of the Tn10 transposase gene fused to signals functional in B. subtilis, these derivatives transposed with a frequency of 10(-6) per element per generation. They had no highly preferred insertion site or region, as judged by restriction analysis of the chromosomal DNA, and generated auxotrophic and sporulation-deficient mutants with a frequency of about 1%. These results suggest that Tn10 derivatives might be a useful genetic tool in B. subtilis and possibly other gram-positive microorganisms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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