Abstract
Potassium pyroantimonate, when used as fixative (saturated or half-saturated, without addition of any conventional fixative) has been demonstrated to produce intracellular precipitates of the insoluble salts of calcium, magnesium, and sodium and to preserve the general cell morphology. In both animal and plant tissues, the electron-opaque antimonate precipitates were found deposited in the nucleus—as well as within the nucleolus—and in the cytoplasm, largely at the site of the ribonucleoprotein particles; the condensed chromatin appeared relatively free of precipitates. The inorganic cations are probably in a loosely bound state since they are not retained by conventional fixatives. The implications of this inorganic cation distribution in the intact cell are discussed in connection with their anionic counterparts, i.e., complexing of cations by fixed anionic charges and the coexistence of a large pool of inorganic orthophosphate anions in the nucleus and nucleolus.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bulger R. E. Use of potassium pyroantimonate in the localization of sodium ions in rat kidney tissue. J Cell Biol. 1969 Jan;40(1):79–94. doi: 10.1083/jcb.40.1.79. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaye G. I., Cole J. D., Donn A. Electron microscopy: sodium localization in normal and ouabain-treated transporting cells. Science. 1965 Nov 26;150(3700):1167–1168. doi: 10.1126/science.150.3700.1167. [DOI] [PubMed] [Google Scholar]
- Kaye G. I., Wheeler H. O., Whitlock R. T., Lane N. Fluid transport in the rabbit gallbladder. A combined physiological and electron microscopic study. J Cell Biol. 1966 Aug;30(2):237–268. doi: 10.1083/jcb.30.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lane B. P., Martin E. Electron probe analysis of cationic species in pyroantimonate precipitates in epon-embedded tissue. J Histochem Cytochem. 1969 Feb;17(2):102–106. doi: 10.1177/17.2.102. [DOI] [PubMed] [Google Scholar]
- Legato M. J., Langer G. A. The subcellular localization of calcium ion in mammalian myocardium. J Cell Biol. 1969 May;41(2):401–423. doi: 10.1083/jcb.41.2.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liau M. C., Perry R. P. Ribosome precursor particles in nucleoli. J Cell Biol. 1969 Jul;42(1):272–283. doi: 10.1083/jcb.42.1.272. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Libanati C. M., Tandler C. J. The distribution of the water-soluble inorganic orthophosphate ions within the cell: accumulation in the nucleus. Electron probe microanalysis. J Cell Biol. 1969 Sep;42(3):754–765. doi: 10.1083/jcb.42.3.754. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Monneron A., Bernhard W. Fine structural organization of the interphase nucleus in some mammalian cells. J Ultrastruct Res. 1969 May;27(3):266–288. doi: 10.1016/s0022-5320(69)80017-1. [DOI] [PubMed] [Google Scholar]
- Nolte A. Elektronenmikroskopische Untersuchungen zum Natrium- und Chloridionentransport in der proximalen Tubuluszelle der Rattenniere. Z Zellforsch Mikrosk Anat. 1966;72(4):562–573. [PubMed] [Google Scholar]
- Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
- Rogers M. E. Ribonucleoprotein particles in the amphibian oocyte nucleus. Possible intermediates in ribosome synthesis. J Cell Biol. 1968 Mar;36(3):421–432. doi: 10.1083/jcb.36.3.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STUDZINSKI G. P. SELECTIVE BINDING OF ZINC BY BASIC PROTEINS OF THE HELA CELL NUCLEOLUS. J Histochem Cytochem. 1965 May-Jun;13:365–375. doi: 10.1177/13.5.365. [DOI] [PubMed] [Google Scholar]
- Spicer S. S., Hardin J. H., Greene W. B. Nuclear precipitates in pyroantimonate-osmium tetroxide-fixed tissues. J Cell Biol. 1968 Oct;39(1):216–221. doi: 10.1083/jcb.39.1.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tandler C. J., Solari A. J. Nucleolar orthophosphate ions. Electron microscope and diffraction studies. J Cell Biol. 1969 Apr;41(1):91–108. doi: 10.1083/jcb.41.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]