Abstract
Rat liver peroxisomes have been separated according to size by zonal sedimentation. A method is described for calculating the size of the particles from their final position in the gradient. Peroxisomes seem biochemically homogeneous throughout their size distribution. 3 hr after injection of tritiated leucine, the specific radioactivity of catalase is the same in peroxisomes of different sizes, and it remains so for up to 1 wk after administration of the precursor. This observation rules out the possibility that peroxisomes have an extended period of independent growth. If individual particles maintain an independent existence, they must be formed very rapidly. The other possible explanation is that peroxisomes exchange material within the liver cell.
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- Anderson N. G., Rutenberg E. Analytical techniques for cell fractions. VII. A simple gradient-forming apparatus. Anal Biochem. 1967 Nov;21(2):259–265. doi: 10.1016/0003-2697(67)90187-x. [DOI] [PubMed] [Google Scholar]
- Anderson N. G., Waters D. A., Fisher W. D., Cline G. B., Nunley C. E., Elrod L. H., Rankin C. T., Jr Analytical techniques for cell fractions. V. Characteristics of the B-XIV and B-XV zonal centrifuge rotors. Anal Biochem. 1967 Nov;21(2):235–252. doi: 10.1016/0003-2697(67)90185-6. [DOI] [PubMed] [Google Scholar]
- Baudhuin P., Beaufay H., De Duve C. Combined biochemical and morphological study of particulate fractions from rat liver. Analysis of preparations enriched in lysosomes or in particles containing urate oxidase, D-amino acid oxidase, and catalase. J Cell Biol. 1965 Jul;26(1):219–243. doi: 10.1083/jcb.26.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beaufay H., Jacques P., Baudhuin P., Sellinger O. Z., Berthet J., De Duve C. Tissue fractionation studies. 18. Resolution of mitochondrial fractions from rat liver into three distinct populations of cytoplasmic particles by means of density equilibration in various gradients. Biochem J. 1964 Jul;92(1):184–205. doi: 10.1042/bj0920184. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
- De Duve C., Baudhuin P. Peroxisomes (microbodies and related particles). Physiol Rev. 1966 Apr;46(2):323–357. doi: 10.1152/physrev.1966.46.2.323. [DOI] [PubMed] [Google Scholar]
- HIGASHI T., PETERS T., Jr STUDIES ON RAT LIVER CATALASE. II. INCORPORATION OF 14-C-LEUCINE INTO CATALASE OF LIVER CELL FRACTIONS IN VIVO. J Biol Chem. 1963 Dec;238:3952–3954. [PubMed] [Google Scholar]
- Leighton F., Poole B., Lazarow P. B., De Duve C. The synthesis and turnover of rat liver peroxisomes. I. Fractionation of peroxisome proteins. J Cell Biol. 1969 May;41(2):521–535. doi: 10.1083/jcb.41.2.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PRICE V. E., STERLING W. R., TARANTOLA V. A., HARTLEY R. W., Jr, RECHCIGL M., Jr The kinetics of catalase synthesis and destruction in vivo. J Biol Chem. 1962 Nov;237:3468–3475. [PubMed] [Google Scholar]
- Poole B. Biogenesis and turnover of rat liver peroxisomes. Ann N Y Acad Sci. 1969 Dec 19;168(2):229–243. doi: 10.1111/j.1749-6632.1969.tb43112.x. [DOI] [PubMed] [Google Scholar]