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. 1970 Jun 1;45(3):598–605. doi: 10.1083/jcb.45.3.598

MEMBRANE SPLITTING IN FREEZE-ETCHING

Covalently Bound Ferritin as a Membrane Marker

Pedro Pinto da Silva 1, Daniel Branton 1
PMCID: PMC2107921  PMID: 4918216

Abstract

The freeze-etch technique was used to observe red blood cell ghosts labeled on both surfaces with covalently bound ferritin. Ferritin molecules were never observed on fracture faces, thus indicating that fracture does not show membrane-surface detail. Subliming away the surrounding ice did expose the ferritin on the membrane surface. These results were consistent with the concept that membranes split during the fracture process of freeze-etching.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Branton D. Fracture faces of frozen membranes. Proc Natl Acad Sci U S A. 1966 May;55(5):1048–1056. doi: 10.1073/pnas.55.5.1048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Branton D., Southworth D. Fracture faces of frozen Chlorella and Saccharomyces cells. Exp Cell Res. 1967 Sep;47(3):648–653. doi: 10.1016/0014-4827(67)90028-6. [DOI] [PubMed] [Google Scholar]
  3. Clark A. W., Branton D. Fracture faces in frozen outer segments from the guinea pig retina. Z Zellforsch Mikrosk Anat. 1968;91(4):586–603. doi: 10.1007/BF00455276. [DOI] [PubMed] [Google Scholar]
  4. DODGE J. T., MITCHELL C., HANAHAN D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
  5. Deamer D. W., Branton D. Fracture planes in an ice-bilayer model membrane system. Science. 1967 Nov 3;158(3801):655–657. doi: 10.1126/science.158.3801.655. [DOI] [PubMed] [Google Scholar]
  6. Haggis G. H. The iron oxide core of the ferritin molecule. J Mol Biol. 1965 Dec;14(2):598–602. doi: 10.1016/s0022-2836(65)80210-8. [DOI] [PubMed] [Google Scholar]
  7. Koehler J. K. Freeze-etching observations on nucleated erythrocytes with special reference to the nuclear and plasma membranes. Z Zellforsch Mikrosk Anat. 1968;85(1):1–17. doi: 10.1007/BF00330582. [DOI] [PubMed] [Google Scholar]
  8. Nelson G. J. Lipid composition of erythrocytes in various mammalian species. Biochim Biophys Acta. 1967 Oct 2;144(2):221–232. doi: 10.1016/0005-2760(67)90152-x. [DOI] [PubMed] [Google Scholar]
  9. Park R. B., Pfeifhofer A. O. Ultrastructural observations on deep-etched thylakoids. J Cell Sci. 1969 Jul;5(1):299–311. doi: 10.1242/jcs.5.1.299. [DOI] [PubMed] [Google Scholar]
  10. SALEM L. The role of long-range forces in the cohesion of lipoproteins. Can J Biochem Physiol. 1962 Sep;40:1287–1298. [PubMed] [Google Scholar]
  11. SCHICK A. F., SINGER S. J. On the formation of covalent linkages between two protein molecules. J Biol Chem. 1961 Sep;236:2477–2485. [PubMed] [Google Scholar]
  12. Staehelin L. A. The interpretation of freeze-etched artificial and bilogical membranes. J Ultrastruct Res. 1968 Feb;22(3):326–347. doi: 10.1016/s0022-5320(68)90025-7. [DOI] [PubMed] [Google Scholar]
  13. Stoeckenius W., Engelman D. M. Current models for the structure of biological membranes. J Cell Biol. 1969 Sep;42(3):613–646. doi: 10.1083/jcb.42.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Weinstein R. S., Bullivant S. The application of freeze-cleaving technics to studies on red blood cell fine structure. Blood. 1967 May;29(5):780–789. [PubMed] [Google Scholar]

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