Abstract
The peroxidatic activity of hemoglobin permitted visualization of its uptake by rat hepatocytes by means of the Graham-Karnovsky 3,3'-diaminobenzidine (DAB) procedure. Lysosomes were visualized by their acid phosphatase, β-glucuronidase, and glucosaminidase activities. When large doses of rat, cow, or human hemoglobin are intravenously injected, or when hemoglobinemia is induced by injection of distilled water, DAB-positive hemoglobin is engulfed by pinocytosis. Pinocytotic vacuoles become digestive vacuoles ("phagolysosomes") by fusion with lysosomes of the dense body type that have moved from their pericanalicular position. By 16–24 hr after even massive amounts of hemoglobin (400 mg/100 g), the protein is barely demonstrable in hepatocytes. At the lowest doses of injected hemoglobin (15 mg/100 g body weight), DAB-positive vacuoles are demonstrable only in the Kupffer cells.
Full Text
The Full Text of this article is available as a PDF (2.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blair O. M., Stenger R. J., Hopkins R. W., Simeone F. A. Hepatocellular ultrastructure in dogs with hypovolemic shock. Lab Invest. 1968 Feb;18(2):172–178. [PubMed] [Google Scholar]
- ERICSSON J. L. ABSORPTION AND DECOMPOSITION OF HOMOLOGOUS HEMOGLOBIN IN RENAL PROXIMAL TUBULAR CELLS. Acta Pathol Microbiol Scand Suppl. 1964;168:SUPPL 168–168:1+. [PubMed] [Google Scholar]
- Goldfischer S. The cytochemical localization of myoglobin in striated muscle of man and walrus. J Cell Biol. 1967 Jul;34(1):398–403. doi: 10.1083/jcb.34.1.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
- Graham R. C., Jr, Kellermeyer R. W. Bovine lactoperoxidase as a cytochemical protein tracer for electron microscopy. J Histochem Cytochem. 1968 Apr;16(4):275–278. doi: 10.1177/16.4.275. [DOI] [PubMed] [Google Scholar]
- HAYASHI M. HISTOCHEMICAL DEMONSTRATION OF N-ACETYL-BETA-GLUCOSAMINIDASE EMPLOYING NAPHTHOL AS-BI N-ACETYL-BETA -GLUCOSAMINIDE AS SUBSTRATE. J Histochem Cytochem. 1965 May-Jun;13:355–360. doi: 10.1177/13.5.355. [DOI] [PubMed] [Google Scholar]
- HAYASHI M., NAKAJIMA Y., FISHMAN W. H. THE CYTOLOGIC DEMONSTRATION OF BETA-GLUCURONIDASE EMPLOYING NAPHTHOL AS-BI GLUCURONIDE AND HEXAZONIUM PARAROSANILIN; A PRELIMINARY REPORT. J Histochem Cytochem. 1964 Apr;12:293–297. doi: 10.1177/12.4.293. [DOI] [PubMed] [Google Scholar]
- Hug G., Schubert W. K. Lysosomes in type II glycogenosis. Changes during administration of extract from Aspergillus niger. J Cell Biol. 1967 Oct;35(1):C1–C6. doi: 10.1083/jcb.35.1.c1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levy E., Slusser R. J., Ruebner B. H. Hepatic changes produced by a single dose of endotoxin in the mouse. Electron microscopy. Am J Pathol. 1968 Feb;52(2):477–502. [PMC free article] [PubMed] [Google Scholar]
- Morell A. G., Irvine R. A., Sternlieb I., Scheinberg I. H., Ashwell G. Physical and chemical studies on ceruloplasmin. V. Metabolic studies on sialic acid-free ceruloplasmin in vivo. J Biol Chem. 1968 Jan 10;243(1):155–159. [PubMed] [Google Scholar]
- OSTROW J. D., JANDL J. H., SCHMID R. The formation of bilirubin from hemoglobin in vivo. J Clin Invest. 1962 Aug;41:1628–1637. doi: 10.1172/JCI104620. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WACHSTEIN M., MEISEL E. DEMONSTRATION OF PEROXIDASE ACTIVITY IN TISSUE SECTIONS. J Histochem Cytochem. 1964 Jul;12:538–544. doi: 10.1177/12.7.538. [DOI] [PubMed] [Google Scholar]
- WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]