Abstract
The number of sperm incorporated into eggs made polyspermic with varying concentrations of nicotine (0.025–0.25%, v/v) appears to be directly related to the concentrations employed. The cortical response is morphologically equivalent to that observed in control preparations. Shortly after their incorporation all of the spermatozoa undergo structural events normally associated with the development of the male pronucleus in monospermic eggs. During the reorganization of the spermatozoa, sperm asters are formed. The number of male pronuclei that initially migrate to and encounter the female pronucleus is usually one to three. When pronuclei come into proximity to one another the surface of the female pronucleus proximal to the advancing male pronuclei flattens and becomes highly convoluted. Subsequently, the pronuclei contact each other and the outer and inner membranes of the pronuclear envelopes fuse, thereby producing the zygote nucleus. The male pronuclei remaining in the zygote after this initial series of pronuclear fusions continue to differentiate, i.e. they enlarge, form nucleolus-like bodies, and undergo further chromatin dispersion. In approximately 90% of the zygotes, all of the remaining male pronuclei progressively migrate to the zygote nucleus and fuse to form one large nucleus by 80 min postinsemination. Mitosis and cleavage of the polyspermic zygote occurs later than in monospermic eggs.
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Selected References
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- ALLEN R. D., HAGSTROM B. E. Some interrelationships among cortical reaction phenomena in the sea urchin egg. Exp Cell Res. 1955;(Suppl 3):1–15. [PubMed] [Google Scholar]
- Anderson E. Oocyte differentiation in the sea urchin, Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction. J Cell Biol. 1968 May;37(2):514–539. doi: 10.1083/jcb.37.2.514. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Anderson W. A. Nuclear and cytoplasmic DNA synthesis during early embryogenesis of Paracentrotus lividus. J Ultrastruct Res. 1969 Jan;26(1):95–110. doi: 10.1016/s0022-5320(69)90038-0. [DOI] [PubMed] [Google Scholar]
- Colwin A. L., Colwin L. H. Behavior of the spermatozoon during sperm-blastomere fusion and its significance for fertilization (Saccoglossus kowalevskii: hemichordata). Z Zellforsch Mikrosk Anat. 1967;78(2):208–220. doi: 10.1007/BF00334763. [DOI] [PubMed] [Google Scholar]
- Gurdon J. B. Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes. J Embryol Exp Morphol. 1968 Nov;20(3):401–414. [PubMed] [Google Scholar]
- Gurdon J. B., Woodland H. R. The cytoplasmic control of nuclear activity in animal development. Biol Rev Camb Philos Soc. 1968 May;43(2):233–267. doi: 10.1111/j.1469-185x.1968.tb00960.x. [DOI] [PubMed] [Google Scholar]
- HAGSTROM B. E., ALLEN R. D. The mechanism of nicotine-induced polyspermy. Exp Cell Res. 1956 Feb;10(1):14–23. doi: 10.1016/0014-4827(56)90065-9. [DOI] [PubMed] [Google Scholar]
- HULTIN E., HAGSTROM B. E. The variability in the fertilization rate. Exp Cell Res. 1956 Apr;10(2):294–308. doi: 10.1016/0014-4827(56)90003-9. [DOI] [PubMed] [Google Scholar]
- HULTIN E. Mechanism of fertilization by rate determinations. Exp Cell Res. 1956 Apr;10(2):286–293. doi: 10.1016/0014-4827(56)90002-7. [DOI] [PubMed] [Google Scholar]
- Hunter R. H. Polyspermic fertilization in pigs during the luteal phase of the estrous cycle. J Exp Zool. 1967 Aug;165(3):451–459. doi: 10.1002/jez.1401650313. [DOI] [PubMed] [Google Scholar]
- JENSEN W. A. OBSERVATIONS ON THE FUSION OF NUCLEI IN PLANTS. J Cell Biol. 1964 Dec;23:669–672. doi: 10.1083/jcb.23.3.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Longo F. J., Anderson E. The fine structure of pronuclear development and fusion in the sea urchin, Arbacia punctulata. J Cell Biol. 1968 Nov;39(2):339–368. doi: 10.1083/jcb.39.2.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROTHSCHILD L. Polyspermy. Q Rev Biol. 1954 Dec;29(4):332–342. doi: 10.1086/400393. [DOI] [PubMed] [Google Scholar]
- ROTHSCHILD, SWANN M. M. The fertilization reaction in the sea-urchin egg; the effect of nicotine. J Exp Biol. 1950 Dec;27(3-4):400–406. doi: 10.1242/jeb.27.3.400. [DOI] [PubMed] [Google Scholar]
- Werle E., Schievelbein H. Activity of nicotine and inactivity of kallikrein and kallidin in aggregation of blood platelets. Nature. 1965 Aug 21;207(999):871–872. doi: 10.1038/207871a0. [DOI] [PubMed] [Google Scholar]
- Zimmerman A. M., Zimmerman S. Action of colcemid in sea urchin eggs. J Cell Biol. 1967 Aug;34(2):483–488. doi: 10.1083/jcb.34.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]