Skip to main content
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1971 Dec 1;51(3):826–836. doi: 10.1083/jcb.51.3.826

BASAL BODIES, BUT NOT CENTRIOLES, IN NAEGLERIA

Chandler Fulton 1, Allan D Dingle 1
PMCID: PMC2108039  PMID: 4942778

Abstract

Amebae of Naegleria gruberi transform into flagellates whose basal bodies have the typical centriole-like structure. The amebae appear to lack any homologous structure, even during mitosis. Basal bodies are constructed during transformation and, in cells transforming synchronously at 25°C, they are first seen about 10 min before flagella are seen. No structural precursor for these basal bodies has been found. These observations are discussed in the light of hypotheses about the continuity of centrioles.

Full Text

The Full Text of this article is available as a PDF (958.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldrich H. C. The ultrastructure of meiosis in three species of Physarum. Mycologia. 1967 Jan-Feb;59(1):127–148. [PubMed] [Google Scholar]
  2. Aldrich H. C. The ultrastructure of mitosis in myxamoebae and plasmodia of Physarum flavicomum. Am J Bot. 1969 Mar;56(3):290–299. [PubMed] [Google Scholar]
  3. Bowers B., Korn E. D. The fine structure of Acanthamoeba castellanii. I. The trophozoite. J Cell Biol. 1968 Oct;39(1):95–111. doi: 10.1083/jcb.39.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dingle A. D., Fulton C. Development of the flagellar apparatus of Naegleria. J Cell Biol. 1966 Oct;31(1):43–54. doi: 10.1083/jcb.31.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dippell R. V. The development of basal bodies in paramecium. Proc Natl Acad Sci U S A. 1968 Oct;61(2):461–468. doi: 10.1073/pnas.61.2.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friedländer M., Wahrman J. The spindle as a basal body distributor. A study in the meiosis of the male silkworm moth, Bombyx mori. J Cell Sci. 1970 Jul;7(1):65–89. doi: 10.1242/jcs.7.1.65. [DOI] [PubMed] [Google Scholar]
  7. Fulton C., Dingle A. D. Appearance of the flagellate phenotype in populations of Naegleria amebae. Dev Biol. 1967 Feb;15(2):165–191. doi: 10.1016/0012-1606(67)90012-7. [DOI] [PubMed] [Google Scholar]
  8. Fulton C., Guerrini A. M. Mitotic synchrony in Nalegleria amebae. Exp Cell Res. 1969 Aug;56(2):194–200. doi: 10.1016/0014-4827(69)90002-0. [DOI] [PubMed] [Google Scholar]
  9. GALL J. G. Centriole replication. A study of spermatogenesis in the snail Viviparus. J Biophys Biochem Cytol. 1961 Jun;10:163–193. doi: 10.1083/jcb.10.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Guttes S., Guttes E., Ellis R. A. Electron microscope study of mitosis in Physarum polycephalum. J Ultrastruct Res. 1968 Mar;22(5):508–529. doi: 10.1016/s0022-5320(68)90038-5. [DOI] [PubMed] [Google Scholar]
  11. Ichida A. A., Fuller M. S. Ultrastructure of mitosis in the aquatic fungus Catenaria anguillulae. Mycologia. 1968 Jan-Feb;60(1):141–155. [PubMed] [Google Scholar]
  12. Kalnins V. I., Porter K. R. Centriole replication during ciliogenesis in the chick tracheal epithelium. Z Zellforsch Mikrosk Anat. 1969;100(1):1–30. doi: 10.1007/BF00343818. [DOI] [PubMed] [Google Scholar]
  13. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lessie P. E., Lovett J. S. Ultrastructural changes during sporangium formation and zoospore differentiation in Blastocladiella Emersonii. Am J Bot. 1968 Feb;55(2):220–236. [PubMed] [Google Scholar]
  15. Longo F. J., Anderson E. Cytological aspects of fertilization in the lamellibranch, Mytilus edulis. I. Polar body formation and development of the female pronucleus. J Exp Zool. 1969 Sep;172(1):69–95. doi: 10.1002/jez.1401720107. [DOI] [PubMed] [Google Scholar]
  16. McManus M. A. Ultrastructure of the somatic nuclear division in the plasmodium of the myxomycete Clastoderma debaryanum. Mycologia. 1968 Mar-Apr;60(2):426–436. [PubMed] [Google Scholar]
  17. Mizukami I., Gall J. Centriole replication. II. Sperm formation in the fern, Marsilea, and the cycad, Zamia. J Cell Biol. 1966 Apr;29(1):97–111. doi: 10.1083/jcb.29.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moser J. W., Kreitner G. L. Centrosome structure in Anthoceros laevis and Marchantia polymorpha. J Cell Biol. 1970 Feb;44(2):454–458. doi: 10.1083/jcb.44.2.454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Murray R. G., Murray A. S., Pizzo A. The fine structure of mitosis in rat thymic lymphocytes. J Cell Biol. 1965 Aug;26(2):601–619. doi: 10.1083/jcb.26.2.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Outka D. E., Kluss B. C. The ameba-to-flagellate transformation in Tetramitus rostratus. II. Microtubular morphogenesis. J Cell Biol. 1967 Nov;35(2):323–346. doi: 10.1083/jcb.35.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Perkins F. O. Formation of centriole and centriole-like structures during meiosis and mitosis in Labyrinthula sp. (Rhizopodea, Labyrinthulida). An electron-microscope study. J Cell Sci. 1970 May;6(3):629–653. doi: 10.1242/jcs.6.3.629. [DOI] [PubMed] [Google Scholar]
  22. RENAUD F. L., SWIFT H. THE DEVELOPMENT OF BASAL BODIES AND FLAGELLA IN ALLOMYCES ARBUSCULUS. J Cell Biol. 1964 Nov;23:339–354. doi: 10.1083/jcb.23.2.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. ROBBINS E., GONATAS N. K. THE ULTRASTRUCTURE OF A MAMMALIAN CELL DURING THE MITOTIC CYCLE. J Cell Biol. 1964 Jun;21:429–463. doi: 10.1083/jcb.21.3.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Robbins E., Jentzsch G., Micali A. The centriole cycle in synchronized HeLa cells. J Cell Biol. 1968 Feb;36(2):329–339. doi: 10.1083/jcb.36.2.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Robinow C. F., Caten C. E. Mitosis in Aspergillus nidulans. J Cell Sci. 1969 Sep;5(2):403–431. doi: 10.1242/jcs.5.2.403. [DOI] [PubMed] [Google Scholar]
  26. Robinow C. F., Marak J. A fiber apparatus in the nucleus of the yeast cell. J Cell Biol. 1966 Apr;29(1):129–151. doi: 10.1083/jcb.29.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. SANBORN E., KOEN P. F., MACNABB J. D., MOORE G. CYTOPLASMIC MICROTUBULES IN MAMMALIAN CELLS. J Ultrastruct Res. 1964 Aug;11:123–138. doi: 10.1016/s0022-5320(64)80097-6. [DOI] [PubMed] [Google Scholar]
  28. SCHUSTER F. AN ELECTRON MICROSCOPE STUDY OF THE AMOEBO-FLAGELLATE, NAEGLERIA GRUBERI (SCHARDINGER). I. THE AMOEBOID AND FLAGELLATE STAGES. J Protozool. 1963 Aug;10:297–313. doi: 10.1111/j.1550-7408.1963.tb01681.x. [DOI] [PubMed] [Google Scholar]
  29. STEMPAK J. G., WARD R. T. AN IMPROVED STAINING METHOD FOR ELECTRON MICROSCOPY. J Cell Biol. 1964 Sep;22:697–701. doi: 10.1083/jcb.22.3.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sachs M. I., Anderson E. A cytological study of artificial parthenogenesis in the sea urchin Arbacia punctulata. J Cell Biol. 1970 Oct;47(1):140–158. doi: 10.1083/jcb.47.1.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sorokin S. P. Reconstructions of centriole formation and ciliogenesis in mammalian lungs. J Cell Sci. 1968 Jun;3(2):207–230. doi: 10.1242/jcs.3.2.207. [DOI] [PubMed] [Google Scholar]
  32. Steinman R. M. An electron microscopic study of ciliogenesis in developing epidermis and trachea in the embryo of Xenopus laevis. Am J Anat. 1968 Jan;122(1):19–55. doi: 10.1002/aja.1001220103. [DOI] [PubMed] [Google Scholar]
  33. Tilney L. G., Porter K. R. Studies on microtubules in Heliozoa. I. The fine structure of Actinosphaerium nucleofilum (Barrett), with particular reference to the axial rod structure. Protoplasma. 1965;60(4):317–344. doi: 10.1007/BF01247886. [DOI] [PubMed] [Google Scholar]
  34. VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Van Assel S., Brachet J. Formation de cytasters dans les oeufs de batraciens sous l'action de l'eau lourde. J Embryol Exp Morphol. 1966 Apr;15(2):143–151. [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES