Abstract
In cultures of duck anterior pituitaries, the synthesis and renewal of the specific secretory protein prolactin and of total newly synthesized tissue proteins were studied. As concerns prolactin, assay of the tissue and culture media hormone content demonstrates de novo synthesis of prolactin in vitro at a constant rate during at least 2 wk. The prolactin content after 1 wk and after 2 wk of culture is the same and is similar to the initial content. The renewal time of this prolactin can be estimated at 28 or 48 hr. As concerns total proteins, the use of a chase after a short pulse of 5 min in the presence of tritiated L-leucine demonstrated that newly synthesized proteins are excreted into the culture medium from 30 min to 1 hr after the beginning of the chase. Therefore, the synthesis and excretion of proteins are two discontinuous phenomena. The migration rate of the total proteins was slower than that of prolactin, indicating that this hormone does not represent more than about half of the newly synthesized proteins. These conclusions are in good agreement with those based on high resolution radioautographic data previously obtained on the same material.
Full Text
The Full Text of this article is available as a PDF (438.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALLEN E. H., SCHWEET R. S. Synthesis of hemoglobin in a cell-free system. I. Properties of the complete system. J Biol Chem. 1962 Mar;237:760–767. [PubMed] [Google Scholar]
- Ectors F., Pasteels J. L., Herlant M. Action de la médroxyprogestérone (Provera) sur l'hypophyse, les glandes mammaires et les ovaires chez la ratte. C R Acad Sci Hebd Seances Acad Sci D. 1966 Dec 19;263(25):1988–1991. [PubMed] [Google Scholar]
- Gourdji D., Tixier-Vidal A. Mise en évidence d'un contrôle hypothalamique stimulant de la prolactive hypophysaire chez le Canard. C R Acad Sci Hebd Seances Acad Sci D. 1966 Jul 11;263(2):162–165. [PubMed] [Google Scholar]
- Gourdji D., Tixier-Vidal A. Variations du contenu hypophysaire en prolactine chez le canard Pékin mâle au cours du cycle sexuel et de la photostimulation expérimentale du testicule. C R Acad Sci Hebd Seances Acad Sci D. 1966 Apr 18;262(16):1746–1749. [PubMed] [Google Scholar]
- Tixier-Vidal A., Gourdji D. Evolution cytologique ultrastructurale de l'hypophyse du canard en culture organotypique. Elaboration autonome de prolactine par les explants. C R Acad Sci Hebd Seances Acad Sci D. 1965 Jul 19;261(3):805–808. [PubMed] [Google Scholar]
- Tixier-Vidal A., Picart R. Etude quantitative par radioautographie au microscope electronique de l'utilisation de la DL-leucine-3H par les cellules de l'hypophyse du canard en culture organotypiqe. J Cell Biol. 1967 Dec;35(3):501–519. doi: 10.1083/jcb.35.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]