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. 1971 Nov 1;51(2):355–368. doi: 10.1083/jcb.51.2.355

NUCLEOLAR-DERIVED RIBONUCLEIC ACID IN CHROMOSOMES, NUCLEAR SAP, AND CYTOPLASM OF CHIRONOMUS TENTANS SALIVARY GLAND CELLS

U Ringborg 1, L Rydlander 1
PMCID: PMC2108124  PMID: 5112649

Abstract

The distribution of monodisperse high molecular weight RNA (38, 30, 28, 23, and 18S RNA) was studied in the salivary gland cells of Chironomus tentans. RNA labeled in vitro and in vivo with tritiated cytidine and uridine was isolated from microdissected nucleoli, chromosomes, nuclear sap, and cytoplasm and analyzed by electrophoresis on agarose-acrylamide composite gels. As shown earlier, the nucleoli contain labeled 38, 30, and 23S RNA. In the chromosomes, labeled 18S RNA was found in addition to the 30 and 23S RNA previously reported. The nuclear sap contains labeled 30 and 18S RNA, and the cytoplasm labeled 28 and 18S RNA. On the basis of the present and earlier analyses, it was concluded that the chromosomal monodisperse high molecular weight RNA fractions (a) show a genuine chromosomal localization and are not due to unspecific contamination, (b) are not artefacts caused by in vitro conditions, but are present also in vivo, and (c) are very likely related to nucleolar and cytoplasmic (pre)ribosomal RNA. The 30 and 23S RNA components are likely to be precursors to 28 and 18S ribosomal RNA. The order of appearance of the monodisperse high molecular weight RNA fractions in the nucleus is in turn and order: (a) nucleolus, (b) chromosomes, and (c) nuclear sap. Since both 23 and 18S RNA are present in the chromosomes, the conversion to 18S RNA may take place there. On the other hand, 30S RNA is only found in the nucleus while 28S RNA can only be detected in the cytoplasm, suggesting that this conversion takes place in connection with the exit of the molecule from the nucleus.

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Selected References

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  1. BROWN D. D., GURDON J. B. ABSENCE OF RIBOSOMAL RNA SYNTHESIS IN THE ANUCLEOLATE MUTANT OF XENOPUS LAEVIS. Proc Natl Acad Sci U S A. 1964 Jan;51:139–146. doi: 10.1073/pnas.51.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birnstiel M., Speirs J., Purdom I., Jones K., Loening U. E. Properties and composition of the isolated ribosomal DNA satellite of Xenopus laevis. Nature. 1968 Aug 3;219(5153):454–463. doi: 10.1038/219454a0. [DOI] [PubMed] [Google Scholar]
  3. Brown D. D., Weber C. S. Gene linkage by RNA-DNA hybridization. II. Arrangement of the redundant gene sequences for 28 s and 18 s ribosomal RNA. J Mol Biol. 1968 Jun 28;34(3):681–697. doi: 10.1016/0022-2836(68)90189-7. [DOI] [PubMed] [Google Scholar]
  4. Daneholt B., Edström J. E., Egyházi E., Lambert B., Ringborg U. Chromosomal RNA synthesis in polytene chromosomes of Chironomus tentans. Chromosoma. 1969;28(4):399–417. doi: 10.1007/BF00284257. [DOI] [PubMed] [Google Scholar]
  5. Daneholt B., Edström J. E. The content of deoxyribonucleic acid in individual polytene chromosomes of Chironomus tentans. Cytogenetics. 1967;6(5):350–356. doi: 10.1159/000154939. [DOI] [PubMed] [Google Scholar]
  6. Darnell J. E., Jr Ribonucleic acids from animal cells. Bacteriol Rev. 1968 Sep;32(3):262–290. doi: 10.1128/br.32.3.262-290.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dingman C. W., Peacock A. C. Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresis. Biochemistry. 1968 Feb;7(2):659–668. doi: 10.1021/bi00842a022. [DOI] [PubMed] [Google Scholar]
  8. Egyházi E., Daneholt B., Edström J. E., Lambert B., Ringborg U. Low molecular weight RNA in cell components of Chironomus tentans salivary glands. J Mol Biol. 1969 Sep 28;44(3):517–532. doi: 10.1016/0022-2836(69)90377-5. [DOI] [PubMed] [Google Scholar]
  9. Ennis H. L. Synthesis of ribonucleic acid in L cells during inhibition of protein synthesis by cycloheximide. Mol Pharmacol. 1966 Nov;2(6):543–557. [PubMed] [Google Scholar]
  10. Geiduschek E. P., Haselkorn R. Messenger RNA. Annu Rev Biochem. 1969;38:647–676. doi: 10.1146/annurev.bi.38.070169.003243. [DOI] [PubMed] [Google Scholar]
  11. Granboulan N., Scherrer K. Visualisation in the electron microscope and size of RNA from animal cells. Eur J Biochem. 1969 May 1;9(1):1–20. doi: 10.1111/j.1432-1033.1969.tb00569.x. [DOI] [PubMed] [Google Scholar]
  12. Grossbach U. Chromosomen-Aktivitat und biochemische Zelldifferenzierung in den Speicheldrüsen von Camptochironomus. Chromosoma. 1969;28(2):136–187. doi: 10.1007/BF00331528. [DOI] [PubMed] [Google Scholar]
  13. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Quagliarotti G., Ritossa F. M. On the arrangement of genes for 28 s and 18 s ribosomal RNA's in Drosophila melanogaster. J Mol Biol. 1968 Aug 28;36(1):57–69. doi: 10.1016/0022-2836(68)90219-2. [DOI] [PubMed] [Google Scholar]
  15. RITOSSA F. M., SPIEGELMAN S. LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER. Proc Natl Acad Sci U S A. 1965 Apr;53:737–745. doi: 10.1073/pnas.53.4.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ringborg U., Daneholt B., Edström J. E., Egyházi E., Lambert B. Electrophoretic characterization of nucleolar RNA from Chironomus tentans salivary gland cells. J Mol Biol. 1970 Jul 28;51(2):327–340. doi: 10.1016/0022-2836(70)90146-4. [DOI] [PubMed] [Google Scholar]
  17. Ringborg U., Daneholt B., Edström J. E., Egyházi E., Rydlander L. Evidence for transport of preribosomal RNA from the nucleolus to the chromosomes in Chironomus tentans salivary gland cells. J Mol Biol. 1970 Aug;51(3):679–686. doi: 10.1016/0022-2836(70)90016-1. [DOI] [PubMed] [Google Scholar]
  18. Vaughan M. H., Jr, Soeiro R., Warner J. R., Darnell J. E., Jr The effects of methionine deprivation on ribosome synthesis in HeLa cells. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1527–1534. doi: 10.1073/pnas.58.4.1527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Willems M., Wagner E., Laing R., Penman S. Base composition of ribosomal RNA precursors in the HeLa cell nucleolus: further evidence of non-conservative processing. J Mol Biol. 1968 Mar 14;32(2):211–220. doi: 10.1016/0022-2836(68)90005-3. [DOI] [PubMed] [Google Scholar]

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