Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1971 Nov 1;51(2):419–432. doi: 10.1083/jcb.51.2.419

THE CELLULAR SITE OF ACTION OF ANGIOTENSIN

John B Richardson 1, Aurèle Beaulnes 1
PMCID: PMC2108140  PMID: 4329616

Abstract

The site of action and the distribution of angiotensin II have been studied in the mouse. A comparison of the ratios of angiotensin-14C and inulin-3H at the time of the pressor effect reveals an extracellular pattern of distribution. Morphological studies were made using angiotensin coupled to exogenous enzymes which can be demonstrated histochemically. Coupling of angiotensin to horseradish peroxidase or cytochrome c, with glutaraldehyde or difluorodinitrodiphenylsulfone (FNPS) as the coupling agent, does not alter the pattern of its vasopressor response or that of its inactivation; nor are differences present between angiotensin and the angiotensin-enzyme complexes in the stimulation of in vitro tissue preparations. Dissociation of the complexes was shown not to occur in vitro, but the possibility of a serum factor splitting the complexes immediately after intravenous injection cannot be excluded. Since these complexes are localized on the endothelium and not on the smooth muscle at the time of maximum hypertension, the endothelium is proposed as the site of action for angiotensin.

Full Text

The Full Text of this article is available as a PDF (1.7 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry. 1969 Jan;6(1):43–52. doi: 10.1016/0019-2791(69)90177-3. [DOI] [PubMed] [Google Scholar]
  2. Bakhle Y. S., Reynard A. M., Vane J. R. Metabolism of the angiotensins in isolated perfused tissues. Nature. 1969 Jun 7;222(5197):956–959. doi: 10.1038/222956a0. [DOI] [PubMed] [Google Scholar]
  3. Bruns R. R., Palade G. E. Studies on blood capillaries. I. General organization of blood capillaries in muscle. J Cell Biol. 1968 May;37(2):244–276. doi: 10.1083/jcb.37.2.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clementi F., Palade G. E. Intestinal capillaries. I. Permeability to peroxidase and ferritin. J Cell Biol. 1969 Apr;41(1):33–58. doi: 10.1083/jcb.41.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Day M. D., Owen D. A. The effect of reserpine on the pressor responses to angiotensin in the conscious cat. Br J Pharmacol. 1970 Jun;39(2):414–427. doi: 10.1111/j.1476-5381.1970.tb12904.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Doyle A. E., Louis W. J., Jerums G., Osborn E. C. Metabolism and blood levels following infusion of a radioactive analog of angiotensin. Am J Physiol. 1968 Jul;215(1):164–168. doi: 10.1152/ajplegacy.1968.215.1.164. [DOI] [PubMed] [Google Scholar]
  7. FURCHGOTT R. F., BHADRAKOM S. Reactions of strips of rabbit aorta to epinephrine, isopropylarterenol, sodium nitrite and other drugs. J Pharmacol Exp Ther. 1953 Jun;108(2):129–143. [PubMed] [Google Scholar]
  8. Giacomelli F., Wiener J., Spiro D. The cellular pathology of experimental hypertension. V. Increased permeability of cerebral arterial vessels. Am J Pathol. 1970 Apr;59(1):133–160. [PMC free article] [PubMed] [Google Scholar]
  9. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  10. Graham R. C., Jr, Limpert S., Kellermeyer R. W. The uptake and transport of exogenous proteins in mouse liver. Ultrastructural cytochemical studies with peroxidase tracers. Lab Invest. 1969 Mar;20(3):298–304. [PubMed] [Google Scholar]
  11. Haas E., Goldblatt H., Lewis L., Gipson E. C. Angiotensin-protective cofactor. Am J Physiol. 1969 Oct;217(4):981–987. doi: 10.1152/ajplegacy.1969.217.4.981. [DOI] [PubMed] [Google Scholar]
  12. Hodge R. L., Ng K. K., Vane J. R. Disappearance of angiotensin from the circulation of the dog. Nature. 1967 Jul 8;215(5097):138–141. doi: 10.1038/215138a0. [DOI] [PubMed] [Google Scholar]
  13. Kalsner S., Nickerson M. A method for the study of mechanisms of drug disposition in smooth muscle. Can J Physiol Pharmacol. 1968 Sep;46(5):719–730. doi: 10.1139/y68-113. [DOI] [PubMed] [Google Scholar]
  14. Karnovsky M. J., Rice D. F. Exogenous cytochrome c as an ultrastructural tracer. J Histochem Cytochem. 1969 Nov;17(11):751–753. doi: 10.1177/17.11.751. [DOI] [PubMed] [Google Scholar]
  15. Karnovsky M. J., Shea S. M. Transcapillary transport by pinocytosis. Microvasc Res. 1970 Oct;2(4):353–360. doi: 10.1016/0026-2862(70)90030-0. [DOI] [PubMed] [Google Scholar]
  16. Karnovsky M. J. The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J Cell Biol. 1967 Oct;35(1):213–236. doi: 10.1083/jcb.35.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Like A. A. The uptake of exogenous peroxidase by the beta cells of the islets of Langerhans. Am J Pathol. 1970 May;59(2):225–246. [PMC free article] [PubMed] [Google Scholar]
  18. MOORE D. H., RUSKA H. The fine structure of capillaries and small arteries. J Biophys Biochem Cytol. 1957 May 25;3(3):457–462. doi: 10.1083/jcb.3.3.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Majno G., Shea S. M., Leventhal M. Endothelial contraction induced by histamine-type mediators: an electron microscopic study. J Cell Biol. 1969 Sep;42(3):647–672. doi: 10.1083/jcb.42.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ng K. K., Vane J. R. Fate of angiotensin I in the circulation. Nature. 1968 Apr 13;218(5137):144–150. doi: 10.1038/218144a0. [DOI] [PubMed] [Google Scholar]
  21. Oparil S., Sanders C. A., Haber E. In-vivo and in-vitro conversion of angiotensin I to angiotensin II in dog blood. Circ Res. 1970 May;26(5):591–599. doi: 10.1161/01.res.26.5.591. [DOI] [PubMed] [Google Scholar]
  22. Peart W. S. C. The renin-angiotensin system. A history and review of the renin-angiotensin system. Proc R Soc Lond B Biol Sci. 1969 Jul 1;173(1032):317–325. doi: 10.1098/rspb.1969.0057. [DOI] [PubMed] [Google Scholar]
  23. Reese T. S., Karnovsky M. J. Fine structural localization of a blood-brain barrier to exogenous peroxidase. J Cell Biol. 1967 Jul;34(1):207–217. doi: 10.1083/jcb.34.1.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rhodin J. A. The ultrastructure of mammalian arterioles and precapillary sphincters. J Ultrastruct Res. 1967 Apr;18(1):181–223. doi: 10.1016/s0022-5320(67)80239-9. [DOI] [PubMed] [Google Scholar]
  25. Rhodin J. A. Ultrastructure of mammalian venous capillaries, venules, and small collecting veins. J Ultrastruct Res. 1968 Dec;25(5):452–500. doi: 10.1016/s0022-5320(68)80098-x. [DOI] [PubMed] [Google Scholar]
  26. Richards F. M., Knowles J. R. Glutaraldehyde as a protein cross-linkage reagent. J Mol Biol. 1968 Oct 14;37(1):231–233. doi: 10.1016/0022-2836(68)90086-7. [DOI] [PubMed] [Google Scholar]
  27. SCHACHTER D., FREINKEL N., SCHWARTZ I. L. Movement of insulin between plasma and interstitial fluid. Am J Physiol. 1950 Mar;160(3):532–535. doi: 10.1152/ajplegacy.1950.160.3.532. [DOI] [PubMed] [Google Scholar]
  28. Shea S. M., Karnovsky M. J., Bossert W. H. Vesicular transport across endothelium: simulation of a diffusion model. J Theor Biol. 1969 Jul;24(1):30–42. doi: 10.1016/s0022-5193(69)80004-4. [DOI] [PubMed] [Google Scholar]
  29. Straus W. Changes in intracellular location of small phagosomes (micropinocytic vesicles) in kidney and liver cells in relation to time after injection and dose of horseradish peroxidase. J Histochem Cytochem. 1967 Jul;15(7):381–393. doi: 10.1177/15.7.381. [DOI] [PubMed] [Google Scholar]
  30. Vane J. R. The release and fate of vaso-active hormones in the circulation. Br J Pharmacol. 1969 Feb;35(2):209–242. doi: 10.1111/j.1476-5381.1969.tb07982.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES