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. 1971 Oct 1;51(1):249–264. doi: 10.1083/jcb.51.1.249

A PARACRYSTALLINE INCLUSION IN NEUROSPORA CRASSA

Induction by Ethidium and Acridine, Isolation, and Characterization

David D Wood 1, David J L Luck 1
PMCID: PMC2108245  PMID: 4329522

Abstract

A paracrystal indistinguishable from the one which occurs in the mitochondrial mutant abnormal-1 can be induced in wild-type Neurospora crassa after growth in either ethidium or euflavine. This paracrystal has been isolated and partially characterized. It appears to be composed of a single polypeptide (mol wt 68,000) which can be reversibly crystallized and dissociated by changes in the pH and ionic strength. When aggregated, the polypeptide forms oligomers which are arranged end-to-end into fibers. During the characterization of the polypeptide, it was found that the polypeptide's electrophoretic and immunological properties could be used as assays. Using these methods it was found that the polypeptide normally accumulates in a soluble form in the cytoplasm of wild-type Neurospora at the end of the log-phase of growth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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