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. 1971 Aug 1;50(2):446–456. doi: 10.1083/jcb.50.2.446

ULTRASTRUCTURAL LOCALIZATION OF CALCITONIN IN THE PARAFOLLICULAR CELLS OF PIG THYROID GLAND WITH CYTOCHROME c-LABELED ANTIBODY FRAGMENTS

P B De Grandi 1, J P Kraehenbuhl 1, M A Campiche 1
PMCID: PMC2108284  PMID: 4107018

Abstract

Parafollicular cells in mammalian thyroid glands are thought to be responsible for the secretion of calcitonin. In this study, calcitonin was localized in pig thyroid gland by an indirect immunocytochemical technique using rabbit antiserum directed against synthetic porcine calcitonin for the first step, and sheep Fab fragments prepared against rabbit Fab and coupled to cytochrome c for the second step. The antigenic determinants of calcitonin were present only in the parafollicular cells, whose secretory granules were heavily labeled. Labeling of the cytoplasmic matrix is thought to indicate a possible leakage of the polypeptide from the granules. A striking observation was the complete absence of labeling in the cisternae of the rough-surfaced endoplasmic reticulum and of the Golgi apparatus. It is concluded that the secretory granules of parafollicular cells contain calcitonin; the mechanism of synthesis of this peptide is not clearly understood.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bussolati G., Pearse A. G. Immunofluorescent localization of calcitonin in the 'C' cells of pig and dog thyroid. J Endocrinol. 1967 Feb;37(2):205–209. doi: 10.1677/joe.0.0370205. [DOI] [PubMed] [Google Scholar]
  2. CARO L. G., PALADE G. E. PROTEIN SYNTHESIS, STORAGE, AND DISCHARGE IN THE PANCREATIC EXOCRINE CELL. AN AUTORADIOGRAPHIC STUDY. J Cell Biol. 1964 Mar;20:473–495. doi: 10.1083/jcb.20.3.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COPP D. H., CAMERON E. C., CHENEY B. A., DAVIDSON A. G., HENZE K. G. Evidence for calcitonin--a new hormone from the parathyroid that lowers blood calcium. Endocrinology. 1962 May;70:638–649. doi: 10.1210/endo-70-5-638. [DOI] [PubMed] [Google Scholar]
  4. COUPLAND R. E., PYPER A. S., HOPWOOD D. A METHOD FOR DIFFERENTIATING BETWEEN NORADRENALINE- AND ADRENALINE-STORING CELLS IN THE LIGHT AND ELECTRON MICROSCOPE. Nature. 1964 Mar 21;201:1240–1242. doi: 10.1038/2011240b0. [DOI] [PubMed] [Google Scholar]
  5. Ekholm R., Ericson L. E. The ultrastructure of the parafollicular cells of the thyroid gland in the rat. J Ultrastruct Res. 1968 Jun;23(5):378–402. doi: 10.1016/s0022-5320(68)80105-4. [DOI] [PubMed] [Google Scholar]
  6. Ericson L. E. Subcellular localization of 5-hydroxtryptamine in the parafollicular cells of the mouse thyroid gland. An autoradiographic study. J Ultrastruct Res. 1970 Apr;31(1):162–177. doi: 10.1016/s0022-5320(70)90152-8. [DOI] [PubMed] [Google Scholar]
  7. Jaim-Etcheverry G., Zieher L. M. Cytochemical localization of monoamine stores in sheep thyroid gland at the electron microscope level. Experientia. 1968 Jun 15;24(6):593–595. doi: 10.1007/BF02153794. [DOI] [PubMed] [Google Scholar]
  8. Jamieson J. D., Palade G. E. Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex. J Cell Biol. 1967 Aug;34(2):577–596. doi: 10.1083/jcb.34.2.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jamieson J. D., Palade G. E. Intracellular transport of secretory proteins in the pancreatic exocrine cell. II. Transport to condensing vacuoles and zymogen granules. J Cell Biol. 1967 Aug;34(2):597–615. doi: 10.1083/jcb.34.2.597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Karnovsky M. J., Rice D. F. Exogenous cytochrome c as an ultrastructural tracer. J Histochem Cytochem. 1969 Nov;17(11):751–753. doi: 10.1177/17.11.751. [DOI] [PubMed] [Google Scholar]
  11. Kracht J., Hachmeister U., Breustedt H. J., Lenke M. Immunohistological studies on thyrocalcitonin in C-cells. Endokrinologie. 1968 Feb;52(5):395–401. [PubMed] [Google Scholar]
  12. Kraehenbuhl J. P., De Grandi P. B., Campiche M. A. Ultrastructural localization of intracellular antigen using enzyme-labeled antibody fragments. J Cell Biol. 1971 Aug;50(2):432–445. doi: 10.1083/jcb.50.2.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lietz H., Schmähling H. U., Zippel H. Veränderungen an den C-Zellen der Rattenschilddrüse bei Hyper- und Hypocalciämie. Histochemische, elektronenoptische und autoradiographische Befunde. Virchows Arch A Pathol Pathol Anat. 1969;348(3):290–305. [PubMed] [Google Scholar]
  14. Lietz H. Zur Ultrastruktur der C-Zellen in der Rattenschilddrüse bei gestörtem Calciumstoffwechsel. Virchows Arch A Pathol Pathol Anat. 1970;350(2):136–149. [PubMed] [Google Scholar]
  15. Matsuzawa T., Kurosumi K. Morphological changes in the parafollicular cells of the rat thyroid glands after administration of calcium shown by electron microscopy. Nature. 1967 Mar 4;213(5079):927–928. doi: 10.1038/213927a0. [DOI] [PubMed] [Google Scholar]
  16. Nunez E. A., Gould R. P., Hamilton D. W., Hayward J. S., Holt S. J. Seasonal changes in the fine structure of the basal granular cells of the bat thyroid. J Cell Sci. 1967 Sep;2(3):401–410. doi: 10.1242/jcs.2.3.401. [DOI] [PubMed] [Google Scholar]
  17. Pearse A. G. Common cytochemical and ultrastructural characteristics of cells producing polypeptide hormones (the APUD series) and their relevance to thyroid and ultimobranchial C cells and calcitonin. Proc R Soc Lond B Biol Sci. 1968 May 14;170(1018):71–80. doi: 10.1098/rspb.1968.0025. [DOI] [PubMed] [Google Scholar]
  18. Stoeckel M. E., Porte A., Canguilhem B. Sur l'ultrastructure des cellules parafolliculaires de la thyroïde du hamster sauvage (Cricetus cricetus) C R Acad Sci Hebd Seances Acad Sci D. 1967 May 22;264(21):2490–2492. [PubMed] [Google Scholar]

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