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. 1971 Sep 1;50(3):737–745. doi: 10.1083/jcb.50.3.737

CENTROSOMES AND MICROTUBULES DURING MEIOSIS IN THE MUSHROOM BOLETUS RUBINELLUS

David J McLaughlin 1
PMCID: PMC2108287  PMID: 4329156

Abstract

The double centrosome in the basidium of Boletus rubinellus has been observed in three planes with the electron microscope at interphase preceding nuclear fusion, at prophase I, and at interphase I. It is composed of two components connected by a band-shaped middle part. At anaphase I a single, enlarged centrosome is found at the spindle pole, which is attached to the cell membrane. Microtubules mainly oriented parallel to the longitudinal axis of the basidium are present at prefusion, prophase I and interphase I. Cytoplasmic microtubules are absent when the spindle is present. The relationship of the centrosome in B. rubinellus to that in other organisms and the role of the cytoplasmic microtubules are discussed.

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Selected References

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  1. Burgess J. Microtubules and cell division in the microspore of Dactylorchis fuschii. Protoplasma. 1970;69(2):253–264. doi: 10.1007/BF01280725. [DOI] [PubMed] [Google Scholar]
  2. Girbardt M. Ultrastructure and dynamics of the moving nucleus. Symp Soc Exp Biol. 1968;22:249–259. [PubMed] [Google Scholar]
  3. Johnson U. G., Porter K. R. Fine structure of cell division in Chlamydomonas reinhardi. Basal bodies and microtubules. J Cell Biol. 1968 Aug;38(2):403–425. doi: 10.1083/jcb.38.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kubai D. F., Ris H. Division in the dinoflagellate Gyrodinium cohnii (Schiller). A new type of nuclear reproduction. J Cell Biol. 1969 Feb;40(2):508–528. doi: 10.1083/jcb.40.2.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lu B. C. Meiosis in Coprinus lagopus: a comparative study with light and electron microscopy. J Cell Sci. 1967 Dec;2(4):529–536. doi: 10.1242/jcs.2.4.529. [DOI] [PubMed] [Google Scholar]
  7. MOLLENHAUER H. H. PLASTIC EMBEDDING MIXTURES FOR USE IN ELECTRON MICROSCOPY. Stain Technol. 1964 Mar;39:111–114. [PubMed] [Google Scholar]
  8. Margulis L. Evolutionary criteria in thallophytes: a radical alternative. Science. 1968 Sep 6;161(3845):1020–1022. doi: 10.1126/science.161.3845.1020. [DOI] [PubMed] [Google Scholar]
  9. McLaughlin D. J. Environmental control of fruitbody development in Boletus rubinellus in axenic culture. Mycologia. 1970 Mar-Apr;62(2):307–331. [PubMed] [Google Scholar]
  10. Motta J. J. Somatica nuclear division in Armillaria Mellea. Mycologia. 1969 Sep-Oct;61(5):873–886. [PubMed] [Google Scholar]
  11. Perkins F. O. Formation of centriole and centriole-like structures during meiosis and mitosis in Labyrinthula sp. (Rhizopodea, Labyrinthulida). An electron-microscope study. J Cell Sci. 1970 May;6(3):629–653. doi: 10.1242/jcs.6.3.629. [DOI] [PubMed] [Google Scholar]
  12. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Robinow C. F., Marak J. A fiber apparatus in the nucleus of the yeast cell. J Cell Biol. 1966 Apr;29(1):129–151. doi: 10.1083/jcb.29.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Westergaard M., von Wettstein D. The nucleolar cycle in an ascomycete. C R Trav Lab Carlsberg. 1970;37(10):195–237. [PubMed] [Google Scholar]

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