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. 1967 May 1;33(2):243–253. doi: 10.1083/jcb.33.2.243

PARTICLE ARRANGEMENTS IN PROPLASTIDS OF TRITICUM VULGARE L. SEEDLINGS

Paul G Bartels 1, T Elliot Weier 1
PMCID: PMC2108352  PMID: 6039369

Abstract

Particles having ribosome-like characteristics are described in proplastids of dark-grown wheat seedlings as the membranes of the prolamellar body become transformed, under the influence of light, into grana and fret membranes. Three arrangements of particles were noted: (1) a random distribution of discrete particles; (2) particles occurring in helices or parallel rows; and (3) particles arranged in rough squares with six to eight particles per side. It is possible that the third type of particle is a cross-section of long parallel rods. A particle ranges in size from 170 to 220 A, those of group three being somewhat smaller. The particulates vary from diamond shaped with smooth surfaces to circular with irregular surfaces. These particles have the characteristics of ribosomes as visualized by the electron microscope: they are preserved by glutaraldehyde and osmium tetroxide, they stain intensely with uranyl acetate, and are digested by RNase. Their properties do not coincide with those of viruses, smog-induced particles, stromacenter particles, or phytoferritin. They are frequently adjacent to membranes but never attached to membranes. The involvement of ribosomes in membrane development is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOARDMAN N. K., FRANCKI R. I., WILDMAN S. G. PROTEIN SYNTHESIS BY CELL-FREE EXTRACTS FROM TOBACCO LEAVES. II. ASSOCIATION OF ACTIVITY WITH CHLOROPLAST RIBOSOMES. Biochemistry. 1965 May;4:872–876. doi: 10.1021/bi00881a012. [DOI] [PubMed] [Google Scholar]
  2. CEDERGREN B., HARARY I. IN VITRO STUDIES ON SINGLE BEATING RAT HEART CELLS. VI. ELECTRON MICROSCOPIC STUDIES OF SINGLE CELLS. J Ultrastruct Res. 1964 Dec;11:428–442. doi: 10.1016/s0022-5320(64)80074-5. [DOI] [PubMed] [Google Scholar]
  3. Cronshaw J., Hoefert L., Esau K. Ultrastructural features of Beta leaves infected with beet yellows virus. J Cell Biol. 1966 Dec;31(3):429–443. doi: 10.1083/jcb.31.3.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dallner G., Siekevitz P., Palade G. E. Biogenesis of endoplasmic reticulum membranes. I. Structural and chemical differentiation in developing rat hepatocyte. J Cell Biol. 1966 Jul;30(1):73–96. doi: 10.1083/jcb.30.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GIBBS S. P. Chloroplast development in Ochromonas danica. J Cell Biol. 1962 Nov;15:343–361. doi: 10.1083/jcb.15.2.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HYDE B. B., HODGE A. J., KAHN A., BIRNSTIEL M. L. STUDIES ON PHYTOFERRITIN. I. IDENTIFICATION AND LOCALIZATION. J Ultrastruct Res. 1963 Oct;59:248–258. doi: 10.1016/s0022-5320(63)80005-2. [DOI] [PubMed] [Google Scholar]
  7. Jacobson A. B., Swift H., Bogorad L. Cytochemical studies concerning the occurrence and distribution of RNA in plastids of Zea mays. J Cell Biol. 1963 Jun;17:557–570. doi: 10.1083/jcb.17.3.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LYTTLETON J. W. Isolation of ribosomes from spinach chloroplasts. Exp Cell Res. 1962 Mar;26:312–317. doi: 10.1016/0014-4827(62)90183-0. [DOI] [PubMed] [Google Scholar]
  9. Lee P. E. Electron microscopy of inclusions associated with wheat streak mosaic virus. J Ultrastruct Res. 1965 Oct;13(3):359–366. doi: 10.1016/s0022-5320(65)80082-x. [DOI] [PubMed] [Google Scholar]
  10. MANILOFF J., MOROWITZ H. J., BARRNETT R. J. STUDIES OF THE ULTRASTRUCTURE AND RIBOSOMAL ARRANGEMENTS OF THE PLEUROPNEUMONIA-LIKE ORGANISM A5969. J Cell Biol. 1965 Apr;25:139–150. doi: 10.1083/jcb.25.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MUEHLETHALER K., FREY-WYSSLING A. [Development and structure of proplastids]. J Biophys Biochem Cytol. 1959 Dec;6:507–512. [PMC free article] [PubMed] [Google Scholar]
  12. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SHALLA T. A. Relations of tobacco mosaic virus and barley stripe mosaic virus to their host cells as revealed by ultrathin tissue-sectioning for the electron microscope. Virology. 1959 Feb;7(2):193–219. doi: 10.1016/0042-6822(59)90187-4. [DOI] [PubMed] [Google Scholar]
  14. Spencer D. Protein synthesis by isolated spinach chloroplasts. Arch Biochem Biophys. 1965 Aug;111(2):381–390. doi: 10.1016/0003-9861(65)90200-6. [DOI] [PubMed] [Google Scholar]
  15. ZAITLIN M., BOARDMAN N. K. The association of tobacco mosaic virus with plastids. I. Isolation of virus from the chloroplast fraction of diseased-leaf homogenates. Virology. 1958 Dec;6(3):743–757. doi: 10.1016/0042-6822(58)90120-x. [DOI] [PubMed] [Google Scholar]

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