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. 1970 Oct 1;47(1):263–275. doi: 10.1083/jcb.47.1.263

ELECTRON MICROSCOPE RADIOAUTOGRAPHIC STUDY OF GLYCOGEN SYNTHESIS IN THE RABBIT RETINA

M M Magalhães 1, Antonio Coimbra 1
PMCID: PMC2108392  PMID: 4935337

Abstract

Glycogen is present in the rabbit retina in monoparticulate form. Beta particles (∼ 229 A) are abundant in Müller cell cytoplasm, particularly in its inner portion, decreasing in number outwards along the cell. They are slightly larger (∼ 250 A) and much scarcer in neurons, though regularly present in the juxtanuclear Golgi region of ganglion cells. When the retina was incubated in a glucose-free medium, it was rapidly depleted of native glycogen. On further incubation in medium containing glucose-3H plus unlabeled glucose, glycogen reappeared in the form of beta particles of the same size and distribution as native ones, while radioautography revealed the appearance of amylase-labile radioactivity in the same locations. This newly formed glycogen was not associated with any particular organelle. The rate of synthesis, as judged from the amount of radioactivity, was high in the inner portion of Müller cells and declined uniformly toward the cell outer end, following a logarithmic gradient. The rate of synthesis was low in ganglion cells, at best approaching values in the outer portion of Müller cells. The concentration of glycogen in the inner portion of Müller cells is consistent with the view that it may be the source of glucose for the anaerobic glycolysis prevailing in the inner retina.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COGAN D. G., KUWABARA T. Tetrazolium studies on the retina. II. Substrate dependent patterns. J Histochem Cytochem. 1959 Sep;7:334–341. doi: 10.1177/7.5.334. [DOI] [PubMed] [Google Scholar]
  2. COHEN L. H., NOELL W. K. Glucose catabolism of rabbit retina before and after development of visual function. J Neurochem. 1960 May;5:253–276. doi: 10.1111/j.1471-4159.1960.tb13363.x. [DOI] [PubMed] [Google Scholar]
  3. Coimbra A., Leblond C. P. Sites of glycogen synthesis in rat liver cells as shown by electron microscope radioautography after administration of glucose-H3. J Cell Biol. 1966 Jul;30(1):151–175. doi: 10.1083/jcb.30.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coimbra A. Radioautographic studies of glycogen synthesis in the striated muscle of rat tongue. Am J Anat. 1969 Mar;124(3):361–377. doi: 10.1002/aja.1001240306. [DOI] [PubMed] [Google Scholar]
  5. Coimbra A. Evaluation of the glycogenolytic effect of alpha-amylase using radioautography and electron microscopy. J Histochem Cytochem. 1966 Dec;14(12):898–906. doi: 10.1177/14.12.898. [DOI] [PubMed] [Google Scholar]
  6. HASTINGS A. B., TENG C. T., NESBETT F. B., SINEX F. M. Studies on carbohydrate metabolism in rat liver slices. I. The effect of cations in the media. J Biol Chem. 1952 Jan;194(1):69–81. [PubMed] [Google Scholar]
  7. HUTCHINSON B. T., KUWABARA T. Phosphorylase and uridine diphosphoglucose glycogen synthetase in the retina. Arch Ophthalmol. 1962 Oct;68:538–545. doi: 10.1001/archopht.1962.00960030542021. [DOI] [PubMed] [Google Scholar]
  8. KUWABARA T., COGAN D. G. Retinal glycogen. Arch Ophthalmol. 1961 Nov;66:680–688. doi: 10.1001/archopht.1961.00960010682013. [DOI] [PubMed] [Google Scholar]
  9. KUWABARA T., COGAN D. G. Tetrzolium studies on the retina: III. Activity of metabolic intermediates and miscellaneous substrates. J Histochem Cytochem. 1960 May;8:214–224. doi: 10.1177/8.3.214. [DOI] [PubMed] [Google Scholar]
  10. Kopriwa B M. A semiautomatic instrument for the radioautographic coating technique. J Histochem Cytochem. 1966 Dec;14(12):923–928. doi: 10.1177/14.12.923. [DOI] [PubMed] [Google Scholar]
  11. Kopriwa B. M. The influence of development on the number and appearance of silver grains in electron microscopic radioautography. J Histochem Cytochem. 1967 Sep;15(9):501–515. doi: 10.1177/15.9.501. [DOI] [PubMed] [Google Scholar]
  12. LESSELL S., KUWABARA T. PHOSPHATASE HISTOCHEMISTRY OF THE EYE. Arch Ophthalmol. 1964 Jun;71:851–860. doi: 10.1001/archopht.1964.00970010867015. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROBERTS N. R., LEWIS C. The quantitative histochemistry of the retina. J Biol Chem. 1956 Jun;220(2):879–892. [PubMed] [Google Scholar]
  14. STROMINGER J. L., LOWRY O. H. The quantitative histochemistry of brain. IV. Lactic, malic, and glutamic dehydrogenases. J Biol Chem. 1955 Apr;213(2):635–646. [PubMed] [Google Scholar]
  15. VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wolfe D. E., Nicholls J. G. Uptake of radioactive glucose and its conversion to glycogen by neurons and glial cells in the leech central nervous system. J Neurophysiol. 1967 Nov;30(6):1593–1609. doi: 10.1152/jn.1967.30.6.1593. [DOI] [PubMed] [Google Scholar]

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