Abstract
Visual cell outer segment renewal was studied in eyes of mutant Royal College of Surgeons (RCS) and Sprague-Dawley (control) rats by a combination of microscopy and radioautography with the light and electron microscopes. RCS and control rats were injected with amino acids-3H at 11 days of age. Radioactive rod outer segment discs were assembled at the outer segment base from radioactive proteins synthesized in the rod inner segments. In controls, all radioactive discs assembled at 11 days of age were displaced the length of the outer segments, removed from outer segment tips, and phagocytized by the pigment epithelium by 8 days after injection. In the RCS rats, disc assembly and displacement resembled controls for the first 3 days after injection. However, as disc assembly continued for some time thereafter, a layer of labeled, disorganized, lamellar debris accumulated between the outer segment tips and the pigment epithelium. The buildup of debris was accompanied by visual cell death. At no time during the study was there evidence for phagocytic activity by the pigment epithelium. 61 days after injection, the layer of debris was the only heavily radioactive component in the retina. In the retina of RCS rats, the outer segment renewal mechanism malfunctions because the pigment epithelium does not fulfill its normal phagocytic role. The end result is visual cell death and blindness.
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Selected References
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- Bourne M. C., Campbell D. A., Tansley K. HEREDITARY DEGENERATION OF THE RAT RETINA. Br J Ophthalmol. 1938 Oct;22(10):613–623. doi: 10.1136/bjo.22.10.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DOWLING J. E., SIDMAN R. L. Inherited retinal dystrophy in the rat. J Cell Biol. 1962 Jul;14:73–109. doi: 10.1083/jcb.14.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall M. O., Bok D., Bacharach A. D. Biosynthesis and assembly of the rod outer segment membrane system. Formation and fate of visual pigment in the frog retina. J Mol Biol. 1969 Oct 28;45(2):397–406. doi: 10.1016/0022-2836(69)90114-4. [DOI] [PubMed] [Google Scholar]
- Herron W. L., Riegel B. W., Myers O. E., Rubin M. L. Retinal dystrophy in the rat--a pigment epithelial disease. Invest Ophthalmol. 1969 Dec;8(6):595–604. [PubMed] [Google Scholar]
- LUCAS D. R., ATTFIELD M., DAVEY J. B. Retinal dystrophy in the rat. J Pathol Bacteriol. 1955 Oct;70(2):469–474. doi: 10.1002/path.1700700225. [DOI] [PubMed] [Google Scholar]
- Lettré H., Paweletz N. Probleme der elektronenmikroskopischen Autoradiographie. Naturwissenschaften. 1966 Jun;53(11):268–271. doi: 10.1007/BF00621640. [DOI] [PubMed] [Google Scholar]
- Peters T., Jr, Ashley C. A. An artefact in radioautography due to binding of free amino acids to tissues by fixatives. J Cell Biol. 1967 Apr;33(1):53–60. doi: 10.1083/jcb.33.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Young R. W., Bok D. Participation of the retinal pigment epithelium in the rod outer segment renewal process. J Cell Biol. 1969 Aug;42(2):392–403. doi: 10.1083/jcb.42.2.392. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Young R. W., Droz B. The renewal of protein in retinal rods and cones. J Cell Biol. 1968 Oct;39(1):169–184. doi: 10.1083/jcb.39.1.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Young R. W. Shedding of discs from rod outer segments in the rhesus monkey. J Ultrastruct Res. 1971 Jan;34(1):190–203. doi: 10.1016/s0022-5320(71)90014-1. [DOI] [PubMed] [Google Scholar]
- Young R. W. The renewal of photoreceptor cell outer segments. J Cell Biol. 1967 Apr;33(1):61–72. doi: 10.1083/jcb.33.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]