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. 1971 Jun 1;49(3):722–730. doi: 10.1083/jcb.49.3.722

STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS

III. Lipid Incorporation into Various Cellular Membranes of Logarithmic Phase Cultures

Yoshinori Nozawa 1, Guy A Thompson Jr 1
PMCID: PMC2108485  PMID: 5092209

Abstract

When 1-14C-palmitic acid is used to pulse label logarithmic cultures of Tetrahymena pyriformis, radioactivity appears in lipids of the various membrane types at vastly differing rates. The microsomes and postmicrosomal supernatant attain a high specific radioactivity within 1 min, while the membranes enveloping the cilia require several hours to reach the microsomal level. A similar pattern is obtained when the tracer is sodium 1-14C-acetate or 8,9-3H-hexadecyl glycerol. In all fractions the phosphonolipid incorporates radioactivity from 14C-palmitate much less rapidly than do the other major phospholipids. The patterns of labeling suggest that new lipids are transported from a cytoplasmic site of synthesis to points of membrane fabrication throughout the cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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