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. 1971 Jun 1;49(3):712–721. doi: 10.1083/jcb.49.3.712

STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS

II. Isolation and Lipid Analysis of Cell Fractions

Yoshinori Nozawa 1, Guy A Thompson Jr 1
PMCID: PMC2108490  PMID: 4933471

Abstract

A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals significant differences in lipid composition. Most noteworthy are the high concentrations of phosphonolipid and tetrahymanol in the surface membranes.

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Selected References

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