Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1990 Dec;172(12):7278–7281. doi: 10.1128/jb.172.12.7278-7281.1990

Evidence for salt-associated restriction pattern modifications in the archaeobacterium Haloferax mediterranei.

G Juez 1, F Rodriguez-Valera 1, N Herrero 1, F J Mojica 1
PMCID: PMC210857  PMID: 1979325

Abstract

DNA restriction pattern modifications were detected when Haloferax mediterranei was grown in low (10%) salt concentrations. After cells were grown again in optimal (25%) salt concentrations, the original pattern was recovered. These salt-associated DNA modifications were revealed with 5% of the 160 DNA fragments cloned and used as probes in hybridization experiments. Patterns obtained when genomic DNA was digested with different restriction enzymes showed that these modifications are related not to insertions or deletions in genome but to modifications of some specific sequences.

Full text

PDF
7278

Images in this article

7279Image
on p.7279
7279Image
on p.7279
7279Image
on p.7279

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Betlach M., Friedman J., Boyer H. W., Pfeifer F. Characterization of a halobacterial gene affecting bacterio-opsin gene expression. Nucleic Acids Res. 1984 Oct 25;12(20):7949–7959. doi: 10.1093/nar/12.20.7949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blackburn E. H., Karrer K. M. Genomic reorganization in ciliated protozoans. Annu Rev Genet. 1986;20:501–521. doi: 10.1146/annurev.ge.20.120186.002441. [DOI] [PubMed] [Google Scholar]
  3. Cedar H. DNA methylation and gene activity. Cell. 1988 Apr 8;53(1):3–4. doi: 10.1016/0092-8674(88)90479-5. [DOI] [PubMed] [Google Scholar]
  4. DasSarma S. Mechanisms of genetic variability in Halobacterium halobium: the purple membrane and gas vesicle mutations. Can J Microbiol. 1989 Jan;35(1):65–72. doi: 10.1139/m89-010. [DOI] [PubMed] [Google Scholar]
  5. DasSarma S., RajBhandary U. L., Khorana H. G. High-frequency spontaneous mutation in the bacterio-opsin gene in Halobacterium halobium is mediated by transposable elements. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2201–2205. doi: 10.1073/pnas.80.8.2201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dassarma S., Halladay J. T., Jones J. G., Donovan J. W., Giannasca P. J., de Marsac N. T. High-frequency mutations in a plasmid-encoded gas vesicle gene in Halobacterium halobium. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6861–6865. doi: 10.1073/pnas.85.18.6861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Doerfler W. DNA methylation and gene activity. Annu Rev Biochem. 1983;52:93–124. doi: 10.1146/annurev.bi.52.070183.000521. [DOI] [PubMed] [Google Scholar]
  8. Hofman J. D., Schalkwyk L. C., Doolittle W. F. ISH51: a large, degenerate family of insertion sequence-like elements in the genome of the archaebacterium, Halobacterium volcanii. Nucleic Acids Res. 1986 Sep 11;14(17):6983–7000. doi: 10.1093/nar/14.17.6983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Holliday R. The inheritance of epigenetic defects. Science. 1987 Oct 9;238(4824):163–170. doi: 10.1126/science.3310230. [DOI] [PubMed] [Google Scholar]
  10. Kuhn I., Stephenson F. H., Boyer H. W., Greene P. J. Positive-selection vectors utilizing lethality of the EcoRI endonuclease. Gene. 1986;42(3):253–263. doi: 10.1016/0378-1119(86)90229-5. [DOI] [PubMed] [Google Scholar]
  11. Lodwick D., Ross H. N., Harris J. E., Almond J. W., Grant W. D. dam methylation in the archaebacteria. J Gen Microbiol. 1986 Nov;132(11):3055–3059. doi: 10.1099/00221287-132-11-3055. [DOI] [PubMed] [Google Scholar]
  12. McConnell D. J., Searcy D. G., Sutcliffe J. G. A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum. Nucleic Acids Res. 1978 Jun;5(6):1729–1739. doi: 10.1093/nar/5.6.1729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Patterson N. H., Pauling C. Evidence for two restriction-modification systems in Halobacterium cutirubrum. J Bacteriol. 1985 Aug;163(2):783–784. doi: 10.1128/jb.163.2.783-784.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pfeifer F., Betlach M. Genome organization in Halobacterium halobium: a 70 kb island of more (AT) rich DNA in the chromosome. Mol Gen Genet. 1985;198(3):449–455. doi: 10.1007/BF00332938. [DOI] [PubMed] [Google Scholar]
  15. Pfeifer F., Blaseio U., Horne M. Genome structure of Halobacterium halobium: plasmid dynamics in gas vacuole deficient mutants. Can J Microbiol. 1989 Jan;35(1):96–100. doi: 10.1139/m89-015. [DOI] [PubMed] [Google Scholar]
  16. Pfeifer F., Friedman J., Boyer H. W., Betlach M. Characterization of insertions affecting the expression of the bacterio-opsin gene in Halobacterium halobium. Nucleic Acids Res. 1984 Mar 12;12(5):2489–2497. doi: 10.1093/nar/12.5.2489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pfeifer F., Weidinger G., Goebel W. Genetic variability in Halobacterium halobium. J Bacteriol. 1981 Jan;145(1):375–381. doi: 10.1128/jb.145.1.375-381.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  19. Sapienza C., Doolittle W. F. Unusual physical organization of the Halobacterium genome. Nature. 1982 Feb 4;295(5848):384–389. doi: 10.1038/295384a0. [DOI] [PubMed] [Google Scholar]
  20. Sapienza C., Rose M. R., Doolittle W. F. High-frequency genomic rearrangements involving archaebacterial repeat sequence elements. Nature. 1982 Sep 9;299(5879):182–185. doi: 10.1038/299182a0. [DOI] [PubMed] [Google Scholar]
  21. Simsek M., DasSarma S., RajBhandary U. L., Khorana H. G. A transposable element from Halobacterium halobium which inactivates the bacteriorhodopsin gene. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7268–7272. doi: 10.1073/pnas.79.23.7268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Weidinger G., Klotz G., Goebel W. A large plasmid from Halobacterium halobium carrying genetic information for gas vacuole formation. Plasmid. 1979 Jul;2(3):377–386. doi: 10.1016/0147-619x(79)90021-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES