Abstract
Previous studies have implicated the reticuloendothelial cells of the liver in certain aspects of steroid metabolism. The similarity in the metabolism of steroids and polycyclic hydrocarbons suggested that the nonparenchymal cells possibly play a role in these areas. The present study presents evidence that at least one of the microsomal NADPH-requirig enzymes, benzpyrene hydroxylase, is present in nonparenchymal cells and, furthermore, is "inducible." In adult rats treated with 3-methylcholanthrene or β-naphthoflavone, the nonparenchymal cells exhibited increases in benzpyrene hydroxylase activity of 17-fold and five-fold, respectively. Treatment with phenobarbital resulted in only a slight increase in enzyme activity. Enzyme activity in parenchymal cells under similar conditions was increased sixfold and fivefold by 3-methylcholanthrene and β-naphthoflavone, respectively, but not by phenobarbital.
Full Text
The Full Text of this article is available as a PDF (483.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAEZ S., SRIKANTIA S. G., CARLETON A. Cellular sites of hepatic ferritin activation and inactivation. Am J Physiol. 1958 Jan;192(1):181–185. doi: 10.1152/ajplegacy.1957.192.1.181. [DOI] [PubMed] [Google Scholar]
- BERLINER D. L., NABORS C. J., Jr, DOUGHERTY T. F. THE ROLE OF HEPATIC AND ADRENAL RETICULOENDOTHELIAL CELLS IN STEROID BIOTRANSFORMATION. J Reticuloendothel Soc. 1964 Jan;1:1–11. [PubMed] [Google Scholar]
- Berry M. N., Friend D. S. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969 Dec;43(3):506–520. doi: 10.1083/jcb.43.3.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Conney A. H. Pharmacological implications of microsomal enzyme induction. Pharmacol Rev. 1967 Sep;19(3):317–366. [PubMed] [Google Scholar]
- DiCarlo F. J., Crew M. C., Young J. E. Biotransformation of pentaerythritol tetranitrate by parenchymal and reticuloendothelial cells of mouse liver. J Reticuloendothel Soc. 1967 Jan;4(1):24–33. [PubMed] [Google Scholar]
- DiCarlo F. J., Haynes L. J., Coutinho C. B., Phillips G. E. Pentobarbital sleeping time and RES stimulation. J Reticuloendothel Soc. 1965 Dec;2(5):367–378. [PubMed] [Google Scholar]
- Gillette J. R. Biochemistry of drug oxidation and reduction by enzymes in hepatic endoplasmic reticulum. Adv Pharmacol. 1966;4:219–261. doi: 10.1016/s1054-3589(08)60100-3. [DOI] [PubMed] [Google Scholar]
- Govier W. C. Reticuloendothelial cells as the site of sulfanilamide acetylation in the rabbit. J Pharmacol Exp Ther. 1965 Nov;150(2):305–308. [PubMed] [Google Scholar]
- Howard R. B., Pesch L. A. Respiratory activity of intact, isolated parenchymal cells from rat liver. J Biol Chem. 1968 Jun 10;243(11):3105–3109. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Mills D. M., Zucker-Franklin D. Electron microscopic study of isolated Kupffer cells. Am J Pathol. 1969 Feb;54(2):147–166. [PMC free article] [PubMed] [Google Scholar]
- Nebert D. W., Gelboin H. V. Substrate-inducible microsomal aryl hydroxylase in mammalian cell culture. I. Assay and properties of induced enzyme. J Biol Chem. 1968 Dec 10;243(23):6242–6249. [PubMed] [Google Scholar]
- ST. GEORGE S., FRIEDMAN M., BYERS S. O. Mass separation of reticuloendothelial and parenchymal cells of rat's liver. Science. 1954 Sep 17;120(3116):463–465. doi: 10.1126/science.120.3116.463. [DOI] [PubMed] [Google Scholar]
- WATTENBERG L. W., LEONG J. L. EFFECTS OF PHENOTHIAZINES ON PROTECTIVE SYSTEMS AGAINST POLYCYCLIC HYDROCARBONS. Cancer Res. 1965 Apr;25:365–370. [PubMed] [Google Scholar]
- Wooles W. R., Borzelleca J. F. Prolongation of barbiturate sleeping time in mice by stimulation of the reticuloendothelial system (RES). J Reticuloendothel Soc. 1966 May;3(1):41–47. [PubMed] [Google Scholar]