Abstract
Attempts were made to test the motile functions of bundles of microfilaments found in baby hamster kidney (BHK-21) cells, by using cytochalasin B (CB). It was found that individual cells respond differently to the drug. These differential effects are quite obvious in both light and electron microscope preparations. Some cells contain normal bundles of microfilaments even after 24 hr in CB, and other cells form muscle-like configurations which also contain arrays of microfilaments. These varied effects suggest the existence of several types of microfilaments in BHK-21 cells, and make the interpretation of the motile role of microfilaments difficult to evaluate at the present time.
Full Text
The Full Text of this article is available as a PDF (1.1 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adelman M. R., Taylor E. W. Further purification and characterization of slime mold myosin and slime mold actin. Biochemistry. 1969 Dec;8(12):4976–4988. doi: 10.1021/bi00840a047. [DOI] [PubMed] [Google Scholar]
- Barrett D., Angelo G. M. Maternal characteristics of hatching enzymes in hybrid sea urchin embryos. Exp Cell Res. 1969 Oct;57(2):159–166. doi: 10.1016/0014-4827(69)90137-2. [DOI] [PubMed] [Google Scholar]
- Carter S. B. Effects of cytochalasins on mammalian cells. Nature. 1967 Jan 21;213(5073):261–264. doi: 10.1038/213261a0. [DOI] [PubMed] [Google Scholar]
- FRANZINI-ARMSTRONG C., PORTER K. R. SARCOLEMMAL INVAGINATIONS CONSTITUTING THE T SYSTEM IN FISH MUSCLE FIBERS. J Cell Biol. 1964 Sep;22:675–696. doi: 10.1083/jcb.22.3.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Follett E. A., Goldman R. D. The occurrence of microvilli during spreading and growth of BHK21-C13 fibroblasts. Exp Cell Res. 1970 Jan;59(1):124–136. doi: 10.1016/0014-4827(70)90631-2. [DOI] [PubMed] [Google Scholar]
- Goldman R. D., Follett E. A. Birefringent filamentous organelle in BHK-21 cells and its possible role in cell spreading and motility. Science. 1970 Jul 17;169(3942):286–288. doi: 10.1126/science.169.3942.286. [DOI] [PubMed] [Google Scholar]
- Goldman R. D. The role of three cytoplasmic fibers in BHK-21 cell motility. I. Microtubules and the effects of colchicine. J Cell Biol. 1971 Dec;51(3):752–762. doi: 10.1083/jcb.51.3.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ishikawa H., Bischoff R., Holtzer H. Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J Cell Biol. 1969 Nov;43(2):312–328. [PMC free article] [PubMed] [Google Scholar]
- Locke M., Krishnan N., McMahon J. T. A routine method for obtaining high contrast without staining sections. J Cell Biol. 1971 Aug;50(2):540–544. doi: 10.1083/jcb.50.2.540. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nachmias V. T., Huxley H. E. Electron microscope observations on actomyosin and actin preparations from Physarum polycephalum, and on their interaction with heavy meromyosin subfragment I from muscle myosin. J Mol Biol. 1970 May 28;50(1):83–90. doi: 10.1016/0022-2836(70)90105-1. [DOI] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STOKER M., MACPHERSON I. SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES. Nature. 1964 Sep 26;203:1355–1357. doi: 10.1038/2031355a0. [DOI] [PubMed] [Google Scholar]
- Sandborn E. B., Duclos S., Messier P. E., Roberge J. J. Atypical intestinal striated muscle in Drosophila melanogaster. J Ultrastruct Res. 1967 Jun;18(5):695–702. doi: 10.1016/s0022-5320(67)80214-4. [DOI] [PubMed] [Google Scholar]
- Sanger J. W., Holtzer S., Holtzer H. Effects of cytochalasin B on muscle cells in tissue culture. Nat New Biol. 1971 Jan 27;229(4):121–123. doi: 10.1038/newbio229121a0. [DOI] [PubMed] [Google Scholar]
- Schroeder T. E. The contractile ring. I. Fine structure of dividing mammalian (HeLa) cells and the effects of cytochalasin B. Z Zellforsch Mikrosk Anat. 1970;109(4):431–449. [PubMed] [Google Scholar]
- Spooner B. S., Wessells N. K. Effects of cytochalasin B upon microfilaments involved in morphogenesis of salivary epithelium. Proc Natl Acad Sci U S A. 1970 Jun;66(2):360–361. doi: 10.1073/pnas.66.2.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spooner B. S., Yamada K. M., Wessells N. K. Microfilaments and cell locomotion. J Cell Biol. 1971 Jun;49(3):595–613. doi: 10.1083/jcb.49.3.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wessells N. K., Spooner B. S., Ash J. F., Bradley M. O., Luduena M. A., Taylor E. L., Wrenn J. T., Yamada K. Microfilaments in cellular and developmental processes. Science. 1971 Jan 15;171(3967):135–143. doi: 10.1126/science.171.3967.135. [DOI] [PubMed] [Google Scholar]
- Wrenn J. T., Wessells N. K. Cytochalasin B: effects upon microfilaments involved in morphogenesis of estrogen-induced glands of oviduct. Proc Natl Acad Sci U S A. 1970 Jul;66(3):904–908. doi: 10.1073/pnas.66.3.904. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamada K. M., Spooner B. S., Wessells N. K. Axon growth: roles of microfilaments and microtubules. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1206–1212. doi: 10.1073/pnas.66.4.1206. [DOI] [PMC free article] [PubMed] [Google Scholar]