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. 1972 Jan 1;52(1):1–14. doi: 10.1083/jcb.52.1.1

CHANGES IN THE FINE STRUCTURE OF THE NEUROMUSCULAR JUNCTION OF THE FROG CAUSED BY BLACK WIDOW SPIDER VENOM

Allen W Clark 1, William P Hurlbut 1, Alexander Mauro 1
PMCID: PMC2108677  PMID: 4536612

Abstract

Application of black widow spider venom to the neuromuscular junction of the frog causes an increase in the frequency of miniature end-plate potentials (min.e.p.p.) and a reduction in the number of synaptic vesicles in the nerve terminal. Shortly after the increase in min.e.p.p. frequency, the presynaptic membrane of the nerve terminal has either infolded or "lifted." Examination of these infoldings or lifts reveals synaptic vesicles in various stages of fusion with the presynaptic membrane. After the supply of synaptic vesicles has been exhausted, the presynaptic membrane returns to its original position directly opposite the end-plate membrane. The terminal contains all of its usual components with the exception of the synaptic vesicles. The only other alteration of the structures making up the neuromuscular junction occurs in the axon leading to the terminal. Instead of completely filling out its Schwann sheath, the axon has pulled away and its axoplasm appears to be denser than the control. The relation of these events to the vesicle hypothesis is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIRKS R., HUXLEY H. E., KATZ B. The fine structure of the neuromuscular junction of the frog. J Physiol. 1960 Jan;150:134–144. doi: 10.1113/jphysiol.1960.sp006378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BIRKS R., KATZ B., MILEDI R. Physiological and structural changes at the amphibian myoneural junction, in the course of nerve degeneration. J Physiol. 1960 Jan;150:145–168. doi: 10.1113/jphysiol.1960.sp006379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bittner G. D., Kennedy D. Quantitative aspects of transmitter release. J Cell Biol. 1970 Dec;47(3):585–592. doi: 10.1083/jcb.47.3.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Braun M., Schmidt R. F. Potential changes recorded from the frog motor nerve terminal during its activation. Pflugers Arch Gesamte Physiol Menschen Tiere. 1966;287(1):56–80. doi: 10.1007/BF00362454. [DOI] [PubMed] [Google Scholar]
  5. Chen I. L., Lee C. Y. Ultrastructural changes in the motor nerve terminals caused by beta-bungarotoxin. Virchows Arch B Cell Pathol. 1970;6(4):318–325. doi: 10.1007/BF02899133. [DOI] [PubMed] [Google Scholar]
  6. Clark A. W., Mauro A., Longenecker H. E., Jr, Hurlbut W. P. Effects of black widow spider venom on the frog neuromuscular junction. Effects on the fine structure of the frog neuromuscular junction. Nature. 1970 Feb 21;225(5234):703–705. doi: 10.1038/225703a0. [DOI] [PubMed] [Google Scholar]
  7. Couteaux R., Pécot-Dechavassine M. Vésicules synaptiques et poches au niveau des "zones actives" de la jonction neuromusculaire. C R Acad Sci Hebd Seances Acad Sci D. 1970 Dec 21;271(25):2346–2349. [PubMed] [Google Scholar]
  8. D'Ajello V., Mauro A., Bettini S. Effect of the venom of the black widow spider, Latrodectus mactans tredecimguttatus, on evoked action potentials in the isolated nerve cord of Periplaneta americana. Toxicon. 1969 Sep;7(2):139–144. doi: 10.1016/0041-0101(69)90076-2. [DOI] [PubMed] [Google Scholar]
  9. DE ROBERTIS E. D., BENNETT H. S. Some features of the submicroscopic morphology of synapses in frog and earthworm. J Biophys Biochem Cytol. 1955 Jan;1(1):47–58. doi: 10.1083/jcb.1.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. DE ROBERTIS E. Submicroscopic morphology and function of the synapse. Exp Cell Res. 1958;14(Suppl 5):347–369. [PubMed] [Google Scholar]
  11. DEL CASTILLO J., KATZ B. Local activity at a depolarized nerve-muscle junction. J Physiol. 1955 May 27;128(2):396–411. doi: 10.1113/jphysiol.1955.sp005315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. FATT P., KATZ B. An analysis of the end-plate potential recorded with an intracellular electrode. J Physiol. 1951 Nov 28;115(3):320–370. doi: 10.1113/jphysiol.1951.sp004675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fahrenbach W. H. The morphology of the eyes of Limulus. II. Ommatidia of the compound eye. Z Zellforsch Mikrosk Anat. 1969;93(4):451–483. doi: 10.1007/BF00338531. [DOI] [PubMed] [Google Scholar]
  14. Gray E. G., Guillery R. W. Synaptic morphology in the normal and degenerating nervous system. Int Rev Cytol. 1966;19:111–182. doi: 10.1016/s0074-7696(08)60566-5. [DOI] [PubMed] [Google Scholar]
  15. Hubbard J. I., Kwanbunbumpen S. Evidence for the vesicle hypothesis. J Physiol. 1968 Feb;194(2):407–420. doi: 10.1113/jphysiol.1968.sp008415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Karnovsky M. J. The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J Cell Biol. 1967 Oct;35(1):213–236. doi: 10.1083/jcb.35.1.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LILEY A. W. An investigation of spontaneous activity at the neuromuscular junction of the rat. J Physiol. 1956 Jun 28;132(3):650–666. doi: 10.1113/jphysiol.1956.sp005555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Longenecker H. E., Jr, Hurlbut W. P., Mauro A., Clark A. W. Effects of black widow spider venom on the frog neuromuscular junction. Effects on end-plate potential, miniature end-plate potential and nerve terminal spike. Nature. 1970 Feb 21;225(5234):701–703. doi: 10.1038/225701a0. [DOI] [PubMed] [Google Scholar]
  19. Longenecker H. E., Jr, Hurlbut W. P., Mauro A., Clark A. W. Effects of black widow spider venom on the frog neuromuscular junction. Effects on end-plate potential, miniature end-plate potential and nerve terminal spike. Nature. 1970 Feb 21;225(5234):701–703. doi: 10.1038/225701a0. [DOI] [PubMed] [Google Scholar]
  20. Miledi R., Slater C. R. On the degeneration of rat neuromuscular junctions after nerve section. J Physiol. 1970 Apr;207(2):507–528. doi: 10.1113/jphysiol.1970.sp009076. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nickel E., Potter L. T. Synaptic vesicles in freeze-etched electric tissue of Torpedo. Brain Res. 1970 Sep 29;23(1):95–100. doi: 10.1016/0006-8993(70)90352-5. [DOI] [PubMed] [Google Scholar]
  22. PALAY S. L. Synapses in the central nervous system. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):193–202. doi: 10.1083/jcb.2.4.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Paggi P., Rossi A. Effect of Latrodectus mactans tredecimguttatus venom on sympathetic ganglion isolated in vitro. Toxicon. 1971 Jul;9(3):265–269. doi: 10.1016/0041-0101(71)90079-1. [DOI] [PubMed] [Google Scholar]
  24. REGER J. F. The fine structure of neuromuscular synapses of gastrocnemii from mouse and frog. Anat Rec. 1958 Jan;130(1):7–23. doi: 10.1002/ar.1091300103. [DOI] [PubMed] [Google Scholar]
  25. ROBERTSON J. D. The ultrastructure of a reptilian myoneural junction. J Biophys Biochem Cytol. 1956 Jul 25;2(4):381–394. doi: 10.1083/jcb.2.4.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tranzer J. P., Thoenen H. An electron microscopic study of selective, acute degeneration of sympathetic nerve terminals after administration of 6-hydroxydopamine. Experientia. 1968 Feb 15;24(2):155–156. doi: 10.1007/BF02146956. [DOI] [PubMed] [Google Scholar]
  27. Whittaker V. P. The nature of the acetylcholine pools in brain tissue. Prog Brain Res. 1969;31:211–222. doi: 10.1016/S0079-6123(08)63240-9. [DOI] [PubMed] [Google Scholar]

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