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. 1972 Apr 1;53(1):66–72. doi: 10.1083/jcb.53.1.66

SEPARATION OF MITOCHONDRIAL MEMBRANES OF NEUROSPORA CRASSA

II. Submitochondrial Localization of the Isoleucine-Valine Biosynthetic Pathway

W E Cassady 1, E H Leiter 1, A Bergquist 1, R P Wagner 1
PMCID: PMC2108690  PMID: 4259051

Abstract

Separation of Neurospora mitochondrial outer membranes from the inner membrane/matrix fraction was effected by digitonin treatment and discontinuous density gradient centrifugation. The solubilization of four isoleucine-valine biosynthetic enzymes was studied as a function of digitonin concentration and time of incubation in the detergent. The kinetics of the appearance of valine biosynthetic function in fractions outside of the inner membrane/matrix fraction, coupled with enzyme solubilization patterns similar to that for the matrix marker, mitochondrial malate dehydrogenase, indicate that the four isoleucine-valine pathway enzymes are localized in the mitochondrial matrix.

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Selected References

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