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. 1972 Oct 1;55(1):179–185. doi: 10.1083/jcb.55.1.179

CYTOCHALASIN B

VI. Competitive Inhibition of Nucleoside Transport by Cultured Novikoff Rat Hepatoma Cells

Peter G W Plagemann 1, Richard D Estensen 1
PMCID: PMC2108743  PMID: 4347250

Abstract

Cytochalasin B competitively inhibits the transport of uridine and thymidine by Novikoff rat hepatoma cells growing in suspension culture with apparent Ki's of 2 and 6 µM, respectively, but has no effect on the intracellular phosphorylation of the nucleosides. Choline transport is not affected by cytochalasin B. Results from pulse-chase experiments indicate that cytochalasin B has no direct effect on the synthesis of RNA, DNA, or uridine diphosphate-sugars. The inhibition of uridine and thymidine incorporation into nucleic acids by cytochalasin B is solely the consequence of the inhibition of nucleoside transport.

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Selected References

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