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. 1972 Oct 1;55(1):104–125. doi: 10.1083/jcb.55.1.104

FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS

Isolation of Nuclear Membranes

Ariane Monneron 1, Günter Blobel 1, George E Palade 1
PMCID: PMC2108744  PMID: 4120072

Abstract

0.3–0.5 M MgCl2 was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55–60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sectioning, freeze etching, and negative staining procedures.

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Selected References

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