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. 1972 Nov 1;55(2):322–327. doi: 10.1083/jcb.55.2.322

CHROMATIN STRUCTURE AND THE CELL DIVISION CYCLE

Actinomycin Binding in Synchronized HeLa Cells

Thoru Pederson 1, Elliott Robbins 1
PMCID: PMC2108799  PMID: 5076779

Abstract

Measurements of actinomycin-3H binding in synchronized HeLa cells reveal that the binding capacity of chromatin decreases progressively during the S phase despite a doubling of nuclear DNA content, reaches a minimal level during G2 and mitosis, and then increases gradually throughout the subsequent G1 interval. Since this pattern was evident in experiments with living cells, ethanol-fixed cells, and isolated nuclei, but not with purified DNA, the actinomycin binding profile may reflect changes in the degree of association between DNA and chromosomal proteins at different stages of the cell cycle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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