Abstract
Uridine diphosphate (UDP) glucuronyltransferase activity in chick liver rises at hatching from near zero to adult levels. This rise will occur prematurely in embryo liver during organ culture. Increase in enzyme activity during organ culture differs with embryo age: in liver from 11-day old embryos it ceases at adult values; in liver from 5-day old embryos it continues to much higher-than-adult levels. Phenobarbital added to culture medium accelerates these rises in enzyme activity and elevates the plateau reached in 11-day embryo liver to that observed in 5-day embryo liver. Kinetic analysis of the changes in enzyme activity induced by phenobarbital during culture suggests that the regulatory mechanisms for enzyme activity are different in 5- and 11-day embryo liver and that these differences reflect developmental changes occurring in ovo.
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Selected References
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- Benzo C. A., Nemeth A. M. Factors controlling development of chick embryo liver cells during organ culture. J Cell Biol. 1971 Feb;48(2):235–247. doi: 10.1083/jcb.48.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berlin C. M., Schimke R. T. Influence of turnover rates on the responses of enzymes to cortisone. Mol Pharmacol. 1965 Sep;1(2):149–156. [PubMed] [Google Scholar]
- Dingman C. W., Peacock A. C. Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresis. Biochemistry. 1968 Feb;7(2):659–668. doi: 10.1021/bi00842a022. [DOI] [PubMed] [Google Scholar]
- Dutton G. J., Ko V. The synthesis of o-aminophenyl glucuronide in several tissues of the domestic fowl, Gallus gallus, during development. Biochem J. 1966 Jun;99(3):550–556. doi: 10.1042/bj0990550. [DOI] [PMC free article] [PubMed] [Google Scholar]
- King E. J. The colorimetric determination of phosphorus. Biochem J. 1932;26(2):292–297. doi: 10.1042/bj0260292. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ko V., Dutton G. J., Nemeth A. M. Development of uridine diphosphate-glucuronyltransferase activity in cultures of chick-embryo liver. Biochem J. 1967 Sep;104(3):991–998. doi: 10.1042/bj1040991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Louis-Ferdinand R. T., Fuller G. C. Suppression of hepatic ribonuclease during phenobarbital stimulation of drug metabolism. Biochem Biophys Res Commun. 1970 Feb 20;38(4):811–816. doi: 10.1016/0006-291x(70)90654-6. [DOI] [PubMed] [Google Scholar]
- OYAMA V. I., EAGLE H. Measurement of cell growth in tissue culture with a phenol reagent (folin-ciocalteau). Proc Soc Exp Biol Med. 1956 Feb;91(2):305–307. doi: 10.3181/00379727-91-22245. [DOI] [PubMed] [Google Scholar]
- STOREY I. D. THE INHIBITION OF THE URIDINE DIPHOSPHATE-TRANSGLUCURONYLASE ACTIVITY OF MOUSE-LIVER HOMOGENATES BY THIOL REAGENTS. Biochem J. 1965 Apr;95:201–208. doi: 10.1042/bj0950201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seifert J., Remmer H. Suppression of synthesis of RNA present in microsomal membranes after administration of phenobarbital. Biochem Pharmacol. 1971 Mar;20(3):553–560. doi: 10.1016/0006-2952(71)90142-0. [DOI] [PubMed] [Google Scholar]
- Skea B. R., Nemeth A. M. Factors influencing premature induction of UDP-glucuronyltransferase activity in cultured chick embryo liver cells. Proc Natl Acad Sci U S A. 1969 Oct;64(2):795–802. doi: 10.1073/pnas.64.2.795. [DOI] [PMC free article] [PubMed] [Google Scholar]