Abstract
The in vitro regression of experimentally induced chromosome puffs was investigated in explanted salivary gland chromosomes of Drosophila hydei. It was observed that the regression of the puffs 2-32A, 2-36A, 2-48C, and 4-81B is accelerated if substrates for the respiratory metabolism are supplied to the cells. A similar effect can be produced by addition of KCN or oligomycin to medium in which intact salivary glands are incubated. The acceleration of puff regression by these substances occurs not only if the puff-inducing stimulus is removed but as well under conditions in which the stimulus is maintained. Regression of the puffs 2-32A, 2-36A, and 4-81B is inhibited if cycloheximide is present in the incubation medium. Chloramphenicol has no effect on puff regression. Measurements on nicotinamide adenine dinucleotide-dehydrogenase activity in homogenates of salivary glands revealed an increase in enzyme activity of 41 %. Maximum increase is attained at 30 min after the induced puffs have reached their maximum size. The increase in enzyme activity does not occur if the glands are kept in a medium containing either actinomycin D or cycloheximide. Chloramphenicol does not inhibit the increase in enzyme activity. The possible relationship between puff activity and its control as a result of changes in the respiratory metabolism is discussed.
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Selected References
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- Ashburner M. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. V. Responses to environmental treatments. Chromosoma. 1970;31(3):356–376. doi: 10.1007/BF00321231. [DOI] [PubMed] [Google Scholar]
- Berendes H. D. Factors involved in the expression of gene activity in polytene chromosomes. Chromosoma. 1968;24(4):418–437. doi: 10.1007/BF00285017. [DOI] [PubMed] [Google Scholar]
- Berendes H. D. The hormone ecdysone as effector of specific changes in the pattern of gene activities of Drosophila hydei. Chromosoma. 1967;22(3):274–293. doi: 10.1007/BF00319878. [DOI] [PubMed] [Google Scholar]
- Boyd J. B., Berendes H. D., Boyd H. Mass preparation of nuclei from the larval salivary glands of Drosophila hydei. J Cell Biol. 1968 Aug;38(2):369–376. doi: 10.1083/jcb.38.2.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ellgaard E. G., Clever U. RNA metabolism during puff induction in Drosophila melanogaster. Chromosoma. 1971;36(1):60–78. doi: 10.1007/BF00326422. [DOI] [PubMed] [Google Scholar]
- Greenberg J. R. Synthesis and properties of ribosomal RNA in Drosophila. J Mol Biol. 1969 Nov 28;46(1):85–98. doi: 10.1016/0022-2836(69)90059-x. [DOI] [PubMed] [Google Scholar]
- KROEGER H. CHEMICAL NATURE OF THE SYSTEM CONTROLLING GENE ACTIVITIES IN INSECT CELLS. Nature. 1963 Dec 21;200:1234–1235. doi: 10.1038/2001234a0. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Leenders H. J., Berendes H. D. The effect of changes in the respiratory metabolism upon genome activity in Drosophila. I. The induction of gene activity. Chromosoma. 1972;37(4):433–444. doi: 10.1007/BF00284892. [DOI] [PubMed] [Google Scholar]
- Poels C. L. Mucopolysaccharide secretion from Drosophila salivary gland cells as a consequence of hormone induced gene activity. Cell Differ. 1972 Jun;1(2):63–78. doi: 10.1016/0045-6039(72)90030-9. [DOI] [PubMed] [Google Scholar]
- RITOSSA F. M. EXPERIMENTAL ACTIVATION OF SPECIFIC LOCI IN POLYTENE CHROMOSOMES OF DROSOPHILA. Exp Cell Res. 1964 Sep;35:601–607. doi: 10.1016/0014-4827(64)90147-8. [DOI] [PubMed] [Google Scholar]
- Shields G., Sang J. H. Characteristics of five cell types appearing during in vitro culture of embryonic material from Drosophila melanogaster. J Embryol Exp Morphol. 1970 Feb;23(1):53–69. [PubMed] [Google Scholar]
- van Breugel F. M. Puff induction in larval salivary gland chromosomes of Drosophila hydei sturtevant. Genetica. 1966;37(1):17–28. doi: 10.1007/BF01547116. [DOI] [PubMed] [Google Scholar]