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. 1988 Mar;170(3):1123–1128. doi: 10.1128/jb.170.3.1123-1128.1988

Localization and enumeration of fimbria-associated adhesins of Bacteroides loescheii.

E I Weiss 1, J London 1, P E Kolenbrander 1, A R Hand 1, R Siraganian 1
PMCID: PMC210881  PMID: 2893786

Abstract

Monoclonal antibodies that specifically inhibit coaggregation between Bacteroides loescheii PK1295 and its two gram-positive partners Streptococcus sanguis 34 and Actinomyces israelii PK14 were used to enumerate and localize two distinct types of fimbria-associated adhesins on the surface of B. loescheii. Binding studies with radiolabeled monoclonal antibodies indicated that a maximum (Bmax calculated from Scatchard plots) of approximately 400 adhesin molecules specific for S. sanguis and 310 adhesin molecules specific for A. israelii reside on the surface of the cell. Immunoelectron microscopy revealed that the adhesins were not an integral part of the fimbrial subunit; rather, they were usually found on the distal portion of the structures arranged in a random fashion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gaastra W., de Graaf F. K. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiol Rev. 1982 Jun;46(2):129–161. doi: 10.1128/mr.46.2.129-161.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hales B. A., Amyes S. G. The transfer of genes encoding production of mannose-resistant haemagglutinating fimbriae from uropathogenic enterobacteria. J Gen Microbiol. 1986 Aug;132(8):2243–2247. doi: 10.1099/00221287-132-8-2243. [DOI] [PubMed] [Google Scholar]
  3. Hinson G., Knutton S., Lam-Po-Tang M. K., McNeish A. S., Williams P. H. Adherence to human colonocytes of an Escherichia coli strain isolated from severe infantile enteritis: molecular and ultrastructural studies of a fibrillar adhesin. Infect Immun. 1987 Feb;55(2):393–402. doi: 10.1128/iai.55.2.393-402.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jones G. W., Isaacson R. E. Proteinaceous bacterial adhesins and their receptors. Crit Rev Microbiol. 1983;10(3):229–260. doi: 10.3109/10408418209113564. [DOI] [PubMed] [Google Scholar]
  5. Klemm P. Fimbrial adhesions of Escherichia coli. Rev Infect Dis. 1985 May-Jun;7(3):321–340. doi: 10.1093/clinids/7.3.321. [DOI] [PubMed] [Google Scholar]
  6. Kolenbrander P. E., Andersen R. N. Multigeneric aggregations among oral bacteria: a network of independent cell-to-cell interactions. J Bacteriol. 1986 Nov;168(2):851–859. doi: 10.1128/jb.168.2.851-859.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lindberg F., Lund B., Johansson L., Normark S. Localization of the receptor-binding protein adhesin at the tip of the bacterial pilus. Nature. 1987 Jul 2;328(6125):84–87. doi: 10.1038/328084a0. [DOI] [PubMed] [Google Scholar]
  8. Lund B., Lindberg F. P., Båga M., Normark S. Globoside-specific adhesins of uropathogenic Escherichia coli are encoded by similar trans-complementable gene clusters. J Bacteriol. 1985 Jun;162(3):1293–1301. doi: 10.1128/jb.162.3.1293-1301.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Moch T., Hoschützky H., Hacker J., Kröncke K. D., Jann K. Isolation and characterization of the alpha-sialyl-beta-2,3-galactosyl-specific adhesin from fimbriated Escherichia coli. Proc Natl Acad Sci U S A. 1987 May;84(10):3462–3466. doi: 10.1073/pnas.84.10.3462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rhen M., Tenhunen J., Väisänen-Rhen V., Pere A., Båga M., Korhonen T. K. Fimbriation and P-antigen recognition of Escherichia coli strains harbouring mutated recombinant plasmids encoding fimbrial adhesins of the uropathogenic E. coli strain KS71. J Gen Microbiol. 1986 Jan;132(1):71–77. doi: 10.1099/00221287-132-1-71. [DOI] [PubMed] [Google Scholar]
  11. Rosenthal H. E. A graphic method for the determination and presentation of binding parameters in a complex system. Anal Biochem. 1967 Sep;20(3):525–532. doi: 10.1016/0003-2697(67)90297-7. [DOI] [PubMed] [Google Scholar]
  12. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  13. Uhlin B. E., Båga M., Göransson M., Lindberg F. P., Lund B., Norgren M., Normark S. Genes determining adhesin formation in uropathogenic Escherichia coli. Curr Top Microbiol Immunol. 1985;118:163–178. doi: 10.1007/978-3-642-70586-1_9. [DOI] [PubMed] [Google Scholar]
  14. Uhlin B. E., Norgren M., Båga M., Normark S. Adhesion to human cells by Escherichia coli lacking the major subunit of a digalactoside-specific pilus-adhesin. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1800–1804. doi: 10.1073/pnas.82.6.1800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weiss E. I., Kolenbrander P. E., London J., Hand A. R., Andersen R. N. Fimbria-associated proteins of Bacteroides loescheii PK1295 mediate intergeneric coaggregations. J Bacteriol. 1987 Sep;169(9):4215–4222. doi: 10.1128/jb.169.9.4215-4222.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weiss E. I., London J., Kolenbrander P. E., Andersen R. N., Fischler C., Siraganian R. P. Characterization of monoclonal antibodies to fimbria-associated adhesins of Bacteroides loescheii PK1295. Infect Immun. 1988 Jan;56(1):219–224. doi: 10.1128/iai.56.1.219-224.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yoshimura F., Takahashi K., Nodasaka Y., Suzuki T. Purification and characterization of a novel type of fimbriae from the oral anaerobe Bacteroides gingivalis. J Bacteriol. 1984 Dec;160(3):949–957. doi: 10.1128/jb.160.3.949-957.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yoshimura F., Takasawa T., Yoneyama M., Yamaguchi T., Shiokawa H., Suzuki T. Fimbriae from the oral anaerobe Bacteroides gingivalis: physical, chemical, and immunological properties. J Bacteriol. 1985 Aug;163(2):730–734. doi: 10.1128/jb.163.2.730-734.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. de Ree J. M., Schwillens P., van den Bosch J. F. Monoclonal antibodies raised against Pap fimbriae recognize minor component(s) involved in receptor binding. Microb Pathog. 1987 Feb;2(2):113–121. doi: 10.1016/0882-4010(87)90103-3. [DOI] [PubMed] [Google Scholar]

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