Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Mar;170(3):1168–1174. doi: 10.1128/jb.170.3.1168-1174.1988

New loci required for Streptomyces coelicolor morphological and physiological differentiation.

W C Champness 1
PMCID: PMC210888  PMID: 3343216

Abstract

Streptomyces coelicolor colonies differentiate both morphologically, producing aerial spore chains, and physiologically, producing antibiotics as secondary metabolites. Single mutations, which block both aspects of differentiation, define bld (bald colony) genes. To identify new bld genes, mutagenized colonies were screened for blocks in the earliest stage of sporulation, the formation of aerial mycelia, and blocks in antibiotic synthesis. The mutations in 12 mutants were mapped; in each strain, the pleiotropic phenotype was due to a single mutation. Seven of the strains contained mutations in known bld loci, bldA and bldB. Three strains contained mutations in a new locus, bldG, and two contained mutations in another new locus, bldH. Like the previously defined bldA mutants, the bldG and bldH mutants were developmentally blocked on glucose. On a variety of carbon sources whose utilization was subject to glucose repression, the developmental blocks were partially relieved for bldG (and bldA) mutants and fully relieved for bldH mutants. These results are compatible with an hypothesis which suggests that there are two alternative controls on S. coelicolor differentiation, one of which is glucose repressible.

Full text

PDF
1168

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chater K. F. A morphological and genetic mapping study of white colony mutants of Streptomyces coelicolor. J Gen Microbiol. 1972 Aug;72(1):9–28. doi: 10.1099/00221287-72-1-9. [DOI] [PubMed] [Google Scholar]
  2. Chater K. F., Bruton C. J., King A. A., Suarez J. E. The expression of Streptomyces and Escherichia coli drug-resistance determinants cloned into the Streptomyces phage phi C31. Gene. 1982 Jul-Aug;19(1):21–32. doi: 10.1016/0378-1119(82)90185-8. [DOI] [PubMed] [Google Scholar]
  3. Hara O., Horinouchi S., Uozumi T., Beppu T. Genetic analysis of A-factor synthesis in Streptomyces coelicolor A3(2) and Streptomyces griseus. J Gen Microbiol. 1983 Sep;129(9):2939–2944. doi: 10.1099/00221287-129-9-2939. [DOI] [PubMed] [Google Scholar]
  4. Hopwood D. A., Wright H. M. CDA is a new chromosomally-determined antibiotic from Streptomyces coelicolor A3(2). J Gen Microbiol. 1983 Dec;129(12):3575–3579. doi: 10.1099/00221287-129-12-3575. [DOI] [PubMed] [Google Scholar]
  5. Horinouchi S., Hara O., Beppu T. Cloning of a pleiotropic gene that positively controls biosynthesis of A-factor, actinorhodin, and prodigiosin in Streptomyces coelicolor A3(2) and Streptomyces lividans. J Bacteriol. 1983 Sep;155(3):1238–1248. doi: 10.1128/jb.155.3.1238-1248.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kirby R., Hopwood D. A. Genetic determination of methylenomycin synthesis by the SCP1 plasmid of Streptomyces coelicolor A3(2). J Gen Microbiol. 1977 Jan;98(1):239–252. doi: 10.1099/00221287-98-1-239. [DOI] [PubMed] [Google Scholar]
  7. Lakey J. H., Lea E. J., Rudd B. A., Wright H. M., Hopwood D. A. A new channel-forming antibiotic from Streptomyces coelicolor A3(2) which requires calcium for its activity. J Gen Microbiol. 1983 Dec;129(12):3565–3573. doi: 10.1099/00221287-129-12-3565. [DOI] [PubMed] [Google Scholar]
  8. Lomovskaya N. D., Mkrtumian N. M., Gostimskaya N. L., Danilenko V. N. Characterization of temperate actinophage phi C31 isolated from Streptomyces coelicolor A3(2). J Virol. 1972 Feb;9(2):258–262. doi: 10.1128/jvi.9.2.258-262.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Losick R., Youngman P., Piggot P. J. Genetics of endospore formation in Bacillus subtilis. Annu Rev Genet. 1986;20:625–669. doi: 10.1146/annurev.ge.20.120186.003205. [DOI] [PubMed] [Google Scholar]
  10. Merrick M. J. A morphological and genetic mapping study of bald colony mutants of Streptomyces coelicolor. J Gen Microbiol. 1976 Oct;96(2):299–315. doi: 10.1099/00221287-96-2-299. [DOI] [PubMed] [Google Scholar]
  11. Piret J. M., Chater K. F. Phage-mediated cloning of bldA, a region involved in Streptomyces coelicolor morphological development, and its analysis by genetic complementation. J Bacteriol. 1985 Sep;163(3):965–972. doi: 10.1128/jb.163.3.965-972.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rudd B. A., Hopwood D. A. A pigmented mycelial antibiotic in Streptomyces coelicolor: control by a chromosomal gene cluster. J Gen Microbiol. 1980 Aug;119(2):333–340. doi: 10.1099/00221287-119-2-333. [DOI] [PubMed] [Google Scholar]
  13. Rudd B. A., Hopwood D. A. Genetics of actinorhodin biosynthesis by Streptomyces coelicolor A3(2). J Gen Microbiol. 1979 Sep;114(1):35–43. doi: 10.1099/00221287-114-1-35. [DOI] [PubMed] [Google Scholar]
  14. Seno E. T., Chater K. F. Glycerol catabolic enzymes and their regulation in wild-type and mutant strains of Streptomyces coelicolor A3(2). J Gen Microbiol. 1983 May;129(5):1403–1413. doi: 10.1099/00221287-129-5-1403. [DOI] [PubMed] [Google Scholar]
  15. Wright L. F., Hopwood D. A. Actinorhodin is a chromosomally-determined antibiotic in Streptomyces coelicolar A3(2). J Gen Microbiol. 1976 Oct;96(2):289–297. doi: 10.1099/00221287-96-2-289. [DOI] [PubMed] [Google Scholar]
  16. Wright L. F., Hopwood D. A. Identification of the antibiotic determined by the SCP1 plasmid of Streptomyces coelicolor A3(2). J Gen Microbiol. 1976 Jul;95(1):96–106. doi: 10.1099/00221287-95-1-96. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES