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. 1973 Feb 1;56(2):500–518. doi: 10.1083/jcb.56.2.500

THE DEVELOPMENT OF MICROBODIES AND PEROXISOMAL ENZYMES IN GREENING BEAN LEAVES

Peter J Gruber 1, Wayne M Becker 1, Eldon H Newcomb 1
PMCID: PMC2108903  PMID: 4682904

Abstract

The ontogeny of leaf microbodies (peroxisomes) has been followed by (a) fixing primary bean leaves at various stages of greening and examining them ultrastructurally, and (b) homogenizing leaves at the same stages and assaying them for three peroxisomal enzymes. A study employing light-grown seedlings showed that when the leaves are still below ground and achlorophyllous, microbodies are present as small organelles (e.g., 0.3 µm in diameter) associated with endoplasmic reticulum, and that after the leaves have turned green and expanded fully, the microbodies occur as much larger organelles (e.g., 1.5 µm in diameter) associated with chloroplasts. Specific activities of the peroxisomal enzymes increase 3- to 10-fold during this period. A second study showed that when etiolated seedlings are transferred to light, the microbodies do not appear to undergo any immediate morphological change, but that by 72 h they have attained approximately the size and enzymatic activity possessed by microbodies in the mature primary leaves of light-grown plants. It is concluded from the ultrastructural observations that leaf microbodies form as small particles and gradually develop into larger ones through contributions from smooth portions of endoplasmic reticulum. In certain aspects, the development of peroxisomes appears analogous to that of chloroplasts. The possibility is examined that microbodies in green leaves may be relatively long-lived organelles.

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Selected References

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  1. Appleman D. CATALASE-CHLOROPHYLL RELATIONSHIP IN BARLEY SEEDLINGS. Plant Physiol. 1952 Jul;27(3):613–621. doi: 10.1104/pp.27.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Breidenbach R. W., Kahn A., Beevers H. Characterization of glyoxysomes from castor bean endosperm. Plant Physiol. 1968 May;43(5):705–713. doi: 10.1104/pp.43.5.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cooper T. G., Beevers H. Beta oxidation in glyoxysomes from castor bean endosperm. J Biol Chem. 1969 Jul 10;244(13):3514–3520. [PubMed] [Google Scholar]
  5. De Duve C., Baudhuin P. Peroxisomes (microbodies and related particles). Physiol Rev. 1966 Apr;46(2):323–357. doi: 10.1152/physrev.1966.46.2.323. [DOI] [PubMed] [Google Scholar]
  6. Essner E. Endoplasmic reticulum and the origin of microbodies in fetal mouse liver. Lab Invest. 1967 Jul;17(1):71–87. [PubMed] [Google Scholar]
  7. Eyster H. C. CATALASE ACTIVITY IN CHLOROPLAST PIGMENT DEFICIENT TYPES OF CORN. Plant Physiol. 1950 Oct;25(4):630–638. doi: 10.1104/pp.25.4.630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Feierabend J., Beevers H. Developmental Studies on Microbodies in Wheat Leaves : II. Ontogeny of Particulate Enzyme Associations. Plant Physiol. 1972 Jan;49(1):33–39. doi: 10.1104/pp.49.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Feierabend J., Beevers H. Developmental studies on microbodies in wheat leaves : I. Conditions influencing enzyme development. Plant Physiol. 1972 Jan;49(1):28–32. doi: 10.1104/pp.49.1.28. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Filner B., Klein A. O. Changes in enzymatic activities in etiolated bean seedling leaves after a brief illumination. Plant Physiol. 1968 Oct;43(10):1587–1596. doi: 10.1104/pp.43.10.1587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Frederick S. E., Newcomb E. H. Cytochemical localization of catalase in leaf microbodies (peroxisomes). J Cell Biol. 1969 Nov;43(2):343–353. doi: 10.1083/jcb.43.2.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Frederick S. E., Newcomb E. H. Microbody-like organelles in leaf cells. Science. 1969 Mar 21;163(3873):1353–1355. doi: 10.1126/science.163.3873.1353. [DOI] [PubMed] [Google Scholar]
  13. Gerhardt B. P., Beevers H. Developmental studies on glyoxysomes in Ricinus endosperm. J Cell Biol. 1970 Jan;44(1):94–102. doi: 10.1083/jcb.44.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hruban Z., Rechcigl M., Jr Microbodies and related particles. Morphology, biochemistry, and physiology. Int Rev Cytol. 1969;(Suppl):1–296. [PubMed] [Google Scholar]
  15. Klein A. O. Persistent photoreversibility of leaf development. Plant Physiol. 1969 Jun;44(6):897–902. doi: 10.1104/pp.44.6.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kleinkopf G. E., Huffaker R. C., Matheson A. Light-induced de Novo Synthesis of Ribulose 1,5-Diphosphate Carboxylase in Greening Leaves of Barley. Plant Physiol. 1970 Sep;46(3):416–418. doi: 10.1104/pp.46.3.416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Lazarow P. B., De Duve C. Intermediates in the biosynthesis of peroxisomal catalase in rat liver. Biochem Biophys Res Commun. 1971 Dec 3;45(5):1198–1204. doi: 10.1016/0006-291x(71)90145-8. [DOI] [PubMed] [Google Scholar]
  19. Locke M., McMahon J. T. The origin and fate of microbodies in the fat body of an insect. J Cell Biol. 1971 Jan;48(1):61–78. doi: 10.1083/jcb.48.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Novikoff P. M., Novikoff A. B. Peroxisomes in absorptive cells of mammalian small intestine. J Cell Biol. 1972 May;53(2):532–560. doi: 10.1083/jcb.53.2.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Poole B., Higashi T., De Duve C. The synthesis and turnover of rat liver peroxisomes. 3. The size distribution of peroxisomes and the incorporation of new catalase. J Cell Biol. 1970 May;45(2):408–415. doi: 10.1083/jcb.45.2.408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Poole B., Leighton F., De Duve C. The synthesis and turnover of rat liver peroxisomes. II. Turnover of peroxisome proteins. J Cell Biol. 1969 May;41(2):536–546. doi: 10.1083/jcb.41.2.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Reddy J., Svoboda D. Microbodies in experimentally altered cells. 8. Continuities between microbodies and their possible biologic significance. Lab Invest. 1971 Jan;24(1):74–81. [PubMed] [Google Scholar]
  24. Rigatuso J. L., Legg P. G., Wood R. L. Microbody formation in regenerating rat liver. J Histochem Cytochem. 1970 Dec;18(12):893–900. doi: 10.1177/18.12.893. [DOI] [PubMed] [Google Scholar]
  25. Scott N. S., Nair H., Smillie R. M. The Effect of Red Irradiation on Plastid Ribosomal RNA Synthesis in Dark-grown Pea Seedlings. Plant Physiol. 1971 Mar;47(3):385–388. doi: 10.1104/pp.47.3.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. TOLBERT N. E., BURRIS R. H. Light activation of the plant enzyme which oxidizes glycolic acid. J Biol Chem. 1950 Oct;186(2):791–804. [PubMed] [Google Scholar]
  27. TOLBERT N. E., COHAN M. S. Activation of glycolic acid oxidase in plants. J Biol Chem. 1953 Oct;204(2):639–648. [PubMed] [Google Scholar]
  28. Tolbert N. E., Oeser A., Yamazaki R. K., Hageman R. H., Kisaki T. A survey of plants for leaf peroxisomes. Plant Physiol. 1969 Jan;44(1):135–147. doi: 10.1104/pp.44.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Tolbert N. E., Yamazaki R. K. Leaf peroxisomes and their relation to photorespiration and photosynthesis. Ann N Y Acad Sci. 1969 Dec 19;168(2):325–341. doi: 10.1111/j.1749-6632.1969.tb43119.x. [DOI] [PubMed] [Google Scholar]
  30. Tolbert N. E., Yamazaki R. K., Oeser A. Localization and properties of hydroxypyruvate and glyoxylate reductases in spinach leaf particles. J Biol Chem. 1970 Oct 10;245(19):5129–5136. [PubMed] [Google Scholar]
  31. Trelease R. N., Becker W. M., Gruber P. J., Newcomb E. H. Microbodies (Glyoxysomes and Peroxisomes) in Cucumber Cotyledons: Correlative Biochemical and Ultrastructural Study in Light- and Dark-grown Seedlings. Plant Physiol. 1971 Oct;48(4):461–475. doi: 10.1104/pp.48.4.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Tsukada H., Mochizuki Y., Konishi T. Morphogenesis and development of microbodies of hepatocytes of rats during pre- and postnatal growth. J Cell Biol. 1968 May;37(2):231–243. doi: 10.1083/jcb.37.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wood R. L., Legg P. G. Peroxidase activity in rat liver microbodies after amino-triazole inhibition. J Cell Biol. 1970 Jun;45(3):576–585. doi: 10.1083/jcb.45.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. van Poucke M., Cerff R., Barthe F., Mohr H. Simultaneous induction of glycolate oxidase and glyoxylate reductase in white mustard seedlings by phytochrome. Naturwissenschaften. 1970 Mar;57(3):132–133. doi: 10.1007/BF00600062. [DOI] [PubMed] [Google Scholar]

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