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. 1973 Mar 1;56(3):787–796. doi: 10.1083/jcb.56.3.787

A ROLE FOR ANIONIC SITES IN EPITHELIAL ARCHITECTURE

Effects of Cationic Polymers on Cell Membrane Structure

P M Quinton 1, C W Philpott 1
PMCID: PMC2108935  PMID: 4687916

Abstract

The effects of several cationic polymers (poly-L-lysines, protamine, and histone) on rabbit gall bladder epithelial cells were studied to explore possible roles for negative sites in the membrane. The tissue was bathed for 30 min at 37°C in Ringer's solutions containing from 0.1 to 100.0 µg/ml of cationic polymers, and subsequently was fixed with 1% OsO4 and examined with the electron microscope. All cationic polymers, at appropriate concentrations, produced similar changes in membrane structure. Adjacent membranes frequently were fused. Membrane structures such as microvilli lost rigidity. Cell membranes showed an apparent increase in permeability as judged by osmotically traumatized cells. These results indicate that fixed anionic sites play significant roles in stabilizing epithelial membrane structures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cook G. M. Glycoproteins in membranes. Biol Rev Camb Philos Soc. 1968 Aug;43(3):363–391. doi: 10.1111/j.1469-185x.1968.tb00964.x. [DOI] [PubMed] [Google Scholar]
  2. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  3. Diamond J. M., Wright E. M. Biological membranes: the physical basis of ion and nonelectrolyte selectivity. Annu Rev Physiol. 1969;31:581–646. doi: 10.1146/annurev.ph.31.030169.003053. [DOI] [PubMed] [Google Scholar]
  4. Franke W. W., Kartenbeck J., Zentgraf H., Scheer U., Falk H. Membrane-to-membrane cross-bridges. A means to orientation and interaction of membrane faces. J Cell Biol. 1971 Dec;51(3):881–888. doi: 10.1083/jcb.51.3.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Glaeser R. M., Mel H. C. Microelectrophoretic and enzymic studies concerning the carbohydrate at the surface of rat erythrocytes. Arch Biochem Biophys. 1966 Jan;113(1):77–82. doi: 10.1016/0003-9861(66)90158-5. [DOI] [PubMed] [Google Scholar]
  6. Green N. M. Possible modes of organization of protein molecules in membranes. Biochem J. 1971 May;122(5):37P–38P. doi: 10.1042/bj1220037p. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kaye G. I., Maenza R. M., Lane N. Cell replication in rabbit gall bladder. An autoradiographic study of epithelial and associated fibroblast renewal in vivo and in vitro. Gastroenterology. 1966 Nov;51(5):670–680. [PubMed] [Google Scholar]
  8. Kemp R. B. Effect of the removal of cell surface sialic acids on cell aggregation in vitro. Nature. 1968 Jun 29;218(5148):1255–1256. doi: 10.1038/2181255a0. [DOI] [PubMed] [Google Scholar]
  9. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mohos S. C., Skoza L. Glomerular sialoprotein. Science. 1969 Jun 27;164(3887):1519–1521. doi: 10.1126/science.164.3887.1519. [DOI] [PubMed] [Google Scholar]
  11. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rappaport C., Howze G. B. Dissociation of adult mouse liver by sodium tetraphenylboron, a potassium complexing agent. Proc Soc Exp Biol Med. 1966 Apr;121(4):1010–1016. doi: 10.3181/00379727-121-30951. [DOI] [PubMed] [Google Scholar]
  13. Rosenberg S. A., Einstein A. B., Jr Sialic acids on the plasma membrane of cultured human lymphoid cells. Chemical aspects and biosynthesis. J Cell Biol. 1972 May;53(2):466–473. doi: 10.1083/jcb.53.2.466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sherbet G. V., Lakshimi M. S., Rao K. V. Characterisation of ionogenic groups and estimation of the net negative electric charge on the surface of cells using natural pH gradients. Exp Cell Res. 1972 Jan;70(1):113–123. doi: 10.1016/0014-4827(72)90188-7. [DOI] [PubMed] [Google Scholar]
  15. Slegers J. F. Ionic secretion by epithelial membranes. Ciba Found Study Group. 1968;32:68–89. [PubMed] [Google Scholar]
  16. Springer G. F. Role of human cell surface structures in interactions between man and microbes. Naturwissenschaften. 1970 Apr;57(4):162–171. doi: 10.1007/BF00592967. [DOI] [PubMed] [Google Scholar]
  17. Tormey J. M., Diamond J. M. The ultrastructural route of fluid transport in rabbit gall bladder. J Gen Physiol. 1967 Sep;50(8):2031–2060. doi: 10.1085/jgp.50.8.2031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Weiss L., Levinson C. Cell electrophoretic mobility and cationic flux. J Cell Physiol. 1969 Feb;73(1):31–36. doi: 10.1002/jcp.1040730105. [DOI] [PubMed] [Google Scholar]
  19. Weiss L. STUDIES ON CELL DEFORMABILITY : I. Effect of Surface Charge. J Cell Biol. 1965 Sep 1;26(3):735–739. doi: 10.1083/jcb.26.3.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weiss L. The cell periphery. Int Rev Cytol. 1969;26:63–105. doi: 10.1016/s0074-7696(08)61634-4. [DOI] [PubMed] [Google Scholar]

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