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. 1973 Mar 1;56(3):697–701. doi: 10.1083/jcb.56.3.697

[6N]METHYL ADENINE IN THE NUCLEAR DNA OF A EUCARYOTE, TETRAHYMENA PYRIFORMIS

Martin A Gorovsky 1, Stanley Hattman 1, Gloria Lorick Pleger 1
PMCID: PMC2108936  PMID: 4631666

Abstract

DNA isolated from macronuclei of the ciliate, Tetrahymena pyriformis, has been found to contain [6N]methyl adenine (MeAde); this represents the first clear demonstration of significant amounts of MeAde in the DNA of a eucaryote. The amounts of macronuclear MeAde differed slightly between different strains of Tetrahymena, with approximately 0.65–0.80% of the adenine bases being methylated. The MeAde content of macronuclear DNA did not seem to vary in different physiological states. The level of MeAde in DNA isolated from micronuclei, on the other hand, was quite low (at least tenfold lower than in macronuclear DNA).

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Selected References

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