Abstract
The "circle analysis" method of Williams (1969) and a new improved method employing hypothetical grains described in the previous paper have been used to analyze the distribution of autoradiographic grains over erythroid bone marrow cells labeled with radioactive iron, 55Fe. The resolution obtainable with this isotope was determined by measuring the distribution of grains about a thin line source. This distribution was also used in calculation of the circle size for the Williams's analysis and the distances of hypothetical grains for the new method. The new method provides estimates for the amount of activity in the regions of condensed and extended nuclear chromatin and for the concentration of isotope at the junction between these two areas. The possible significance of activity in this junctional region is discussed.
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- BACHMANN L., SALPETER M. M. AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE; A QUANTITATIVE EVALUATION. Lab Invest. 1965 Jun;14:1041–1053. [PubMed] [Google Scholar]
- DAVIES H. G. Structure in nucleated erythrocytes. J Biophys Biochem Cytol. 1961 Mar;9:671–687. doi: 10.1083/jcb.9.3.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FORBERG S., ODEBLAD E., SOEREMARK R., ULLBERG S. AUTORADIOGRAPHY WITH ISOTOPES EMITTING INTERNAL CONVERSION ELECTRONS AND AUGER ELECTRONS. Acta Radiol Ther Phys Biol. 1964 Aug;2:241–262. doi: 10.1080/02841866409134060. [DOI] [PubMed] [Google Scholar]
- HAMMEL C. L., BESSMAN S. P. HEMOGLOBIN SYNTHESIS IN AVIAN ERYTHROCYTES. J Biol Chem. 1964 Jul;239:2228–2238. [PubMed] [Google Scholar]
- KARNOVSKY M. J. Simple methods for "staining with lead" at high pH in electron microscopy. J Biophys Biochem Cytol. 1961 Dec;11:729–732. doi: 10.1083/jcb.11.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Milner G. R., Hayhoe F. G. Ultrastructural localization of nucleic acid synthesis in human blood cells. Nature. 1968 May 25;218(5143):785–787. doi: 10.1038/218785a0. [DOI] [PubMed] [Google Scholar]
- Orlic D. The use of 55Fe in high-resolution radioautography of developing red cells. J Cell Biol. 1968 Oct;39(1):201–208. doi: 10.1083/jcb.39.1.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Salpeter M. M., Salpeter E. E. Resolution in electron microscope radioautography. II. Carbon 14. J Cell Biol. 1971 Aug;50(2):324–332. doi: 10.1083/jcb.50.2.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Small J. V., Davies H. G. The haemoglobin in the condensed chromatin of mature amphibian erythrocytes: a further study. J Cell Sci. 1970 Jul;7(1):15–33. doi: 10.1242/jcs.7.1.15. [DOI] [PubMed] [Google Scholar]
- TEALE F. W. Cleavage of the haem-protein link by acid methylethylketone. Biochim Biophys Acta. 1959 Oct;35:543–543. doi: 10.1016/0006-3002(59)90407-x. [DOI] [PubMed] [Google Scholar]
- TOOZE J., DAVIES H. G. The occurrence and possible significance of haemoglobin in the chromosomal regions of mature erythrocyte nuclei of the newt Triturus cristatus cristatus. J Cell Biol. 1963 Mar;16:501–511. doi: 10.1083/jcb.16.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tarmy E. M., Kaplan N. O. Chemical characterization of D-lactate dehydrogenase from Escherichia coli B. J Biol Chem. 1968 May 25;243(10):2579–2586. [PubMed] [Google Scholar]