Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1973 May 1;57(2):345–359. doi: 10.1083/jcb.57.2.345

PEROXISOMES IN INNER ADRENOCORTICAL CELLS OF FETAL AND ADULT GUINEA PIGS

Virginia H Black 1, Bruce I Bogart 1
PMCID: PMC2108982  PMID: 4633170

Abstract

Abundant membrane-bounded granules, 0.1–0.45 µm in diameter, occur among the elements of the smooth-surfaced endoplasmic reticulum in zona fasciculata and zona reticularis adrenocortical cells of guinea pigs. Acid phosphatase cannot be cytochemically demonstrated in them, and they are therefore distinct from lysosomes. Incubation in medium containing 3,3'-diaminobenzidine results in dense staining of the granules, identifying them as peroxisomes. These small peroxisomes increase in number as fetal adrenocortical cells differentiate, and they appear to arise from dilated regions of endoplasmic reticulum. They maintain interconnections with the smooth endoplasmic reticulum and with one another.

Full Text

The Full Text of this article is available as a PDF (1.7 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams E. C., Hertig A. T. Studies on the human corpus luteum. II. Observations on the ultrastructure of luteal cells during pregnancy. J Cell Biol. 1969 Jun;41(3):716–735. doi: 10.1083/jcb.41.3.716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ahlabo I., Barnard T. Observations on peroxisomes in brown adipose tissue of the rat. J Histochem Cytochem. 1971 Nov;19(11):670–675. doi: 10.1177/19.11.670. [DOI] [PubMed] [Google Scholar]
  3. BELT W. D. The origin of adrenal cortical mitochondria and liposomes: a preliminary report. J Biophys Biochem Cytol. 1958 May 25;4(3):337–340. doi: 10.1083/jcb.4.3.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BOYD G. S. Effect of linoleate and estrogen on cholesterol metabolism. Fed Proc. 1962 Jul-Aug;21(4):86–92. [PubMed] [Google Scholar]
  5. Baudhuin P., Beaufay H., De Duve C. Combined biochemical and morphological study of particulate fractions from rat liver. Analysis of preparations enriched in lysosomes or in particles containing urate oxidase, D-amino acid oxidase, and catalase. J Cell Biol. 1965 Jul;26(1):219–243. doi: 10.1083/jcb.26.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Beard M. E. Identification of peroxisomes in the rat adrenal cortex. J Histochem Cytochem. 1972 Mar;20(3):173–179. doi: 10.1177/20.3.173. [DOI] [PubMed] [Google Scholar]
  7. Beard M. E., Novikoff A. B. Distribution of peroxisomes (microbodies) in the nephron of the rat: a cytochemical study. J Cell Biol. 1969 Aug;42(2):501–518. doi: 10.1083/jcb.42.2.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Berchtold J. P. Contribution à l'étude ultrastructurale des cellules interrénales de Salamandra salamandra L. (amphibien urodèle). I. Conditions normales. Z Zellforsch Mikrosk Anat. 1969;102(3):357–375. [PubMed] [Google Scholar]
  9. Black V. H. Gonocytes in fetal guinea pig testes: phagocytosis of degenerating gonocytes by Sertoli cells. Am J Anat. 1971 Aug;131(4):415–426. doi: 10.1002/aja.1001310403. [DOI] [PubMed] [Google Scholar]
  10. CHRISTENSEN A. K., FAWCETT D. W. The normal fine structure of opossum testicular interstitial cells. J Biophys Biochem Cytol. 1961 Mar;9:653–670. doi: 10.1083/jcb.9.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Chang C. H., Schiller B., Goldfischer S. Small cytoplasmic bodies in the loop of Henle and distal convoluted tubule that resemble peroxisomes. J Histochem Cytochem. 1971 Jan;19(1):56–62. doi: 10.1177/19.1.56. [DOI] [PubMed] [Google Scholar]
  12. Christensen A. K., Fawcett D. W. The fine structure of testicular interstitial cells in mice. Am J Anat. 1966 Mar;118(2):551–571. doi: 10.1002/aja.1001180214. [DOI] [PubMed] [Google Scholar]
  13. Crisp T. M., Dessouky D. A., Denys F. R. The fine structure of the human corpus luteum of early pregnancy and during the progestational phase of the mestrual cycle. Am J Anat. 1970 Jan;127(1):37–69. doi: 10.1002/aja.1001270105. [DOI] [PubMed] [Google Scholar]
  14. De Duve C., Baudhuin P. Peroxisomes (microbodies and related particles). Physiol Rev. 1966 Apr;46(2):323–357. doi: 10.1152/physrev.1966.46.2.323. [DOI] [PubMed] [Google Scholar]
  15. ENDERS A. C. Observations on the fine structure of lutein cells. J Cell Biol. 1962 Jan;12:101–113. doi: 10.1083/jcb.12.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ericsson J. L., Trump B. F. Electron microscopic studies of the epithelium of the proximal tubule of the rat kidney. 3. Microbodies, multivesicular bodies, and the golgi apparatus. Lab Invest. 1966 Oct;15(10):1610–1633. [PubMed] [Google Scholar]
  17. Essner E. Endoplasmic reticulum and the origin of microbodies in fetal mouse liver. Lab Invest. 1967 Jul;17(1):71–87. [PubMed] [Google Scholar]
  18. GANSLER H., ROUILLER C. Modifications physiologiques et pathologiques du chondriome; etude au microscope électronique. Schweiz Z Pathol Bakteriol. 1956;19(2):217–243. [PubMed] [Google Scholar]
  19. Gillim S. W., Christensen A. K., McLennan C. E. Fine structure of the human menstrual corpus luteum at its stage of maximum secretory activity. Am J Anat. 1969 Dec;126(4):409–427. doi: 10.1002/aja.1001260403. [DOI] [PubMed] [Google Scholar]
  20. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  21. HRUBAN Z., SWIFT H. URICASE: LOCALIZATION IN HEPATIC MICROBODIES. Science. 1964 Dec 4;146(3649):1316–1318. doi: 10.1126/science.146.3649.1316. [DOI] [PubMed] [Google Scholar]
  22. Johannisson E. The foetal adrenal cortex in the human. Its ultrastructure at different stages of development and in different functional states. Acta Endocrinol (Copenh) 1968;58(Suppl):7+–7+. [PubMed] [Google Scholar]
  23. Kuhn C. Particles resembling microbodies in normal and neoplastic perianal glands of dogs. Z Zellforsch Mikrosk Anat. 1968;90(4):554–562. doi: 10.1007/BF00339503. [DOI] [PubMed] [Google Scholar]
  24. Kupfer D., Orrenius S. Characteristics of guinea pig liver and adrenal monooxygenase systems. Mol Pharmacol. 1970 May;6(3):221–230. [PubMed] [Google Scholar]
  25. Lazarow P. B., De Duve C. Intermediates in the biosynthesis of peroxisomal catalase in rat liver. Biochem Biophys Res Commun. 1971 Dec 3;45(5):1198–1204. doi: 10.1016/0006-291x(71)90145-8. [DOI] [PubMed] [Google Scholar]
  26. Legg P. G., Wood R. L. New observations on microbodies. A cytochemical study on CPIB-treated rat liver. J Cell Biol. 1970 Apr;45(1):118–129. doi: 10.1083/jcb.45.1.118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Leighton F., Poole B., Lazarow P. B., De Duve C. The synthesis and turnover of rat liver peroxisomes. I. Fractionation of peroxisome proteins. J Cell Biol. 1969 May;41(2):521–535. doi: 10.1083/jcb.41.2.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Long J. A., Jones A. L. Alterations in fine structure of the opossum adrenal cortex following sodium deprivation. Anat Rec. 1970 Jan;166(1):1–25. doi: 10.1002/ar.1091660102. [DOI] [PubMed] [Google Scholar]
  29. Magalhães M. C., Magalhães M. M. Ultrastructural alterations produced in rat adrenal by aminoglutethimide. A stereologic and cytochemical study. Endocrinology. 1972 Feb;90(2):444–452. doi: 10.1210/endo-90-2-444. [DOI] [PubMed] [Google Scholar]
  30. Magalhäes M. M., Magalhäes M. C. Microbodies (peroxisomes) in rat adrenal cortex. J Ultrastruct Res. 1971 Dec;37(5):563–573. doi: 10.1016/s0022-5320(71)80025-4. [DOI] [PubMed] [Google Scholar]
  31. McNutt N. S., Jones A. L. Observations on the ultrastructure of cytodifferentiation in the human fetal adrenal cortex. Lab Invest. 1970 Jun;22(6):513–527. [PubMed] [Google Scholar]
  32. Nickerson P. A., Brownie A. C., Skelton F. R. An electron microscopic study of the regenerating adrenal gland during the development of adrenal regeneration hypertension. Am J Pathol. 1969 Nov;57(2):335–364. [PMC free article] [PubMed] [Google Scholar]
  33. Novikoff A. B., Goldfischer S. Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine. J Histochem Cytochem. 1969 Oct;17(10):675–680. doi: 10.1177/17.10.675. [DOI] [PubMed] [Google Scholar]
  34. Novikoff P. M., Novikoff A. B. Peroxisomes in absorptive cells of mammalian small intestine. J Cell Biol. 1972 May;53(2):532–560. doi: 10.1083/jcb.53.2.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Petrik P. Fine structural identification of peroxisomes in mouse and rat bronchiolar and alveolar epithelium. J Histochem Cytochem. 1971 Jun;19(6):339–348. doi: 10.1177/19.6.339. [DOI] [PubMed] [Google Scholar]
  37. Poole B., Higashi T., De Duve C. The synthesis and turnover of rat liver peroxisomes. 3. The size distribution of peroxisomes and the incorporation of new catalase. J Cell Biol. 1970 May;45(2):408–415. doi: 10.1083/jcb.45.2.408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Poole B., Leighton F., De Duve C. The synthesis and turnover of rat liver peroxisomes. II. Turnover of peroxisome proteins. J Cell Biol. 1969 May;41(2):536–546. doi: 10.1083/jcb.41.2.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. RHODIN J. Electron microscopy of the kidney. Am J Med. 1958 May;24(5):661–675. doi: 10.1016/0002-9343(58)90373-5. [DOI] [PubMed] [Google Scholar]
  40. Reddy J., Svoboda D. Microbodies (peroxisomes) identification in interstitial cells of the testis. J Histochem Cytochem. 1972 Feb;20(2):140–142. doi: 10.1177/20.2.140. [DOI] [PubMed] [Google Scholar]
  41. Reddy J., Svoboda D. Microbodies in experimentally altered cells. 8. Continuities between microbodies and their possible biologic significance. Lab Invest. 1971 Jan;24(1):74–81. [PubMed] [Google Scholar]
  42. Rennels E. G. Observations on the ultrastructure of luteal cells from PMS and PMS-HCG treated immature rats. Endocrinology. 1966 Aug;79(2):373–386. doi: 10.1210/endo-79-2-373. [DOI] [PubMed] [Google Scholar]
  43. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. SABATINI D. D., DE ROBERTIS E. D. Ultrastructural zonation of adrenocortex in the rat. J Biophys Biochem Cytol. 1961 Jan;9:105–119. doi: 10.1083/jcb.9.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. TAPPEL A. L. The mechanism of the oxidation of unsaturated fatty acids catalyzed by hematin compounds. Arch Biochem Biophys. 1953 Jun;44(2):378–395. doi: 10.1016/0003-9861(53)90056-3. [DOI] [PubMed] [Google Scholar]
  46. Tsukada H., Mochizuki Y., Fujiwara S. The nucleoids of rat liver cell microbodies. Fine structure and enzymes. J Cell Biol. 1966 Mar;28(3):449–460. doi: 10.1083/jcb.28.3.449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Tsukada H., Mochizuki Y., Konishi T. Morphogenesis and development of microbodies of hepatocytes of rats during pre- and postnatal growth. J Cell Biol. 1968 May;37(2):231–243. doi: 10.1083/jcb.37.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Vigil E. L. Cytochemical and developmental changes in microbodies (glyoxysomes) and related organelles of castor bean endosperm. J Cell Biol. 1970 Sep;46(3):435–454. doi: 10.1083/jcb.46.3.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  50. Williams N. E., Luft J. H. Use of a nitrogen mustard derivative in fixation for electron microscopy and observations on the ultrastructure of Tetrahymena. J Ultrastruct Res. 1968 Nov;25(3):271–292. doi: 10.1016/s0022-5320(68)80074-7. [DOI] [PubMed] [Google Scholar]
  51. Wood R. L., Legg P. G. Peroxidase activity in rat liver microbodies after amino-triazole inhibition. J Cell Biol. 1970 Jun;45(3):576–585. doi: 10.1083/jcb.45.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  52. de Duve C. The peroxisome: a new cytoplasmic organelle. Proc R Soc Lond B Biol Sci. 1969 Apr 15;173(1030):71–83. [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES