Abstract
Fluorescent and red light environments generate greatly different patterns of pigmentation and morphology in Fremyella diplosiphon. Most strikingly, red-illuminated cultures contain no measurable C-phycoerythrin and have a mean filament length about 10 times shorter than fluorescent-illuminated cultures. C-phycoerythrin behaves as a photoinducible constituent of this alga. Spectrophotometric and immunochemical procedures were devised so that C-phycoerythrin metabolism could be studied quantitatively with [14C]-phenylalanine pulse-chased cultures. Transfer of red-illuminated cultures to fluorescent light initiates C-phycoerythrin production by essentially de novo synthesis. C-phycoerythrin is not degraded to any significant extent in cultures continuously illuminated with fluorescent light. Transfer of fluorescent-illuminated cultures to red light causes an abrupt cessation of C-phycoerythrin synthesis. The C-phycoerythrin content of cultures adapting to red light decreases and subsequently becomes constant. Loss of C-phycoerythrin is not brought about by metabolic degradation, but rather by a decrease in mean filament length which is effected by transcellular breakage. In this experimental system, light influences intracellular C-phycoerythrin levels by regulating the rate of synthesis of the chromoprotein.
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Selected References
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- Bennett A., Bogorad L. Properties of subunits and aggregates of blue-green algal biliproteins. Biochemistry. 1971 Sep 14;10(19):3625–3634. doi: 10.1021/bi00795a022. [DOI] [PubMed] [Google Scholar]
- DUYSENS L. N. M. Transfer of light energy within the pigment systems present in photosynthesizing cells. Nature. 1951 Sep 29;168(4274):548–550. doi: 10.1038/168548a0. [DOI] [PubMed] [Google Scholar]
- FRENCH C. S., YOUNG V. K. The fluorescence spectra of red algae and the transfer of energy from phycoerythrin to phycocyanin and chlorophyll. J Gen Physiol. 1952 Jul;35(6):873–890. doi: 10.1085/jgp.35.6.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAXO F. T., BLINKS L. R. Photosynthetic action spectra of marine algae. J Gen Physiol. 1950 Mar;33(4):389–422. doi: 10.1085/jgp.33.4.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOGNESS D. S., COHN M., MONOD J. Studies on the induced synthesis of beta-galactosidase in Escherichia coli: the kinetics and mechanism of sulfur incorporation. Biochim Biophys Acta. 1955 Jan;16(1):99–116. doi: 10.1016/0006-3002(55)90188-8. [DOI] [PubMed] [Google Scholar]
- KOSHLAND M. E., ENGLBERGER F. M., GADDONE S. M. The effect of iodination on the binding of haptene to arsonic antibody. J Immunol. 1962 Oct;89:517–524. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Scheibe J. Photoreversible pigment: occurrence in a blue-green alga. Science. 1972 Jun 2;176(4038):1037–1039. doi: 10.1126/science.176.4038.1037. [DOI] [PubMed] [Google Scholar]
- Schulze I. T., Colowick S. P. The modification of yeast hexokinases by proteases and its relationship to the dissociation of hexokinase into subunits. J Biol Chem. 1969 May 10;244(9):2306–2316. [PubMed] [Google Scholar]
- Simon R. D. Cyanophycin Granules from the Blue-Green Alga Anabaena cylindrica: A Reserve Material Consisting of Copolymers of Aspartic Acid and Arginine. Proc Natl Acad Sci U S A. 1971 Feb;68(2):265–267. doi: 10.1073/pnas.68.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tishler P. V., Epstein C. J. A convenient method of preparing polyacrylamide gels for liquid scintillation spectrometry. Anal Biochem. 1968 Jan;22(1):89–98. doi: 10.1016/0003-2697(68)90262-5. [DOI] [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]