Abstract
The fine structure of newborn and fetal mouse liver and of newborn kidney cells homozygous for any of three albino alleles known to have multiple biochemical effects was investigated. Electron microscope studies of mutant cells revealed dilation and vesiculation of the rough endoplasmic reticulum in parenchymal liver cells, as well as dilation and other anomalies of the Golgi apparatus. These abnormalities were observed in all newborn mutants but never in littermate controls. Although they were most pronounced in liver parenchymal cells, they were found also to a lesser degree in kidney cells, but they were absent altogether in other cell types of the mutant newborn. Homozygous fetuses showed similar anomalies in the liver at 19 days of gestational age. In one of the alleles studied, mutant liver parenchymal cells were found to be abnormal as early as the 18th day of gestation. There appears to be a striking parallelism between the biochemical defects and those of the cellular membranes in homozygous mutant newborn and fetuses. Although the specific nature of the mutational effect on membrane structure remains unknown, the results are compatible with the assumption that a mutationally caused defect in a membrane component interferes with a mechanism vital in the integration of morphological and biochemical differentiation.
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Selected References
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- Erickson R. P., Gluecksohn-Waelsch S., Cori C. F. Glucose-6-phosphatase deficiency caused by radiation-induced alleles at the albino locus in the mouse. Proc Natl Acad Sci U S A. 1968 Feb;59(2):437–444. doi: 10.1073/pnas.59.2.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gluecksohn-Waelsch S., Cori C. F. Glucose 6-phosphatase deficiency: mechanisms of genetic control and biochemistry. Biochem Genet. 1970 Feb;4(1):195–201. doi: 10.1007/BF00484030. [DOI] [PubMed] [Google Scholar]
- Hugon J. S., Maestracci D., Ménard D. Glucose 6-phosphatase activity in the intestinal epithelium of the mouse. J Histochem Cytochem. 1971 Sep;19(9):515–525. doi: 10.1177/19.9.515. [DOI] [PubMed] [Google Scholar]
- Jézéquel A. M., Arakawa K., Steiner J. W. The fine structure of the normal, neonatal mouse liver. Lab Invest. 1965 Nov;14(11):1894–1930. [PubMed] [Google Scholar]
- Kanamura S. Ultrastructural localization of glucose-6-phosphatase activity in proximal convoluted tubule cells of rat kidney. Histochemie. 1971;28(4):288–295. doi: 10.1007/BF00702634. [DOI] [PubMed] [Google Scholar]
- LAZARUS S. S., BARDEN H. SPECIFICITY AND ULTRASTRUCTURAL LOCALIZATION OF PANCREATIC B CELL GLUCOSE-6-PHOSPHATASE. Diabetes. 1965 Mar;14:146–156. doi: 10.2337/diab.14.3.146. [DOI] [PubMed] [Google Scholar]
- Maunsbach A. B. The influence of different fixatives and fixation methods on the ultrastructure of rat kidney proximal tubule cells. I. Comparison of different perfusion fixation methods and of glutaraldehyde, formaldehyde and osmium tetroxide fixatives. J Ultrastruct Res. 1966 Jun;15(3):242–282. doi: 10.1016/s0022-5320(66)80109-0. [DOI] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Redman C. M., Cherian M. G. The secretory pathways of rat serum glycoproteins and albumin. Localization of newly formed proteins within the endoplasmic reticulum. J Cell Biol. 1972 Feb;52(2):231–245. doi: 10.1083/jcb.52.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smuckler E. A., Arcasoy M. Structural and functional changes of the endoplasmic reticulum of hepatic parenchymal cells. Int Rev Exp Pathol. 1969;7:305–418. [PubMed] [Google Scholar]
- Thorndike J., Trigg M. J., Stockert R., Gluecksohn-Waelsch S., Cori C. F. Multiple biochemical effects of a series of x-ray induced mutations at the albino locus in the mouse. Biochem Genet. 1973 May;9(1):25–39. doi: 10.1007/BF00485588. [DOI] [PubMed] [Google Scholar]