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. 1973 Dec 1;59(3):559–572. doi: 10.1083/jcb.59.3.559

PERMEABILITY OF SERTOLI CELL TIGHT JUNCTIONS TO LANTHANUM AFTER LIGATION OF DUCTUS DEFERENS AND DUCTULI EFFERENTES

William B Neaves 1
PMCID: PMC2109111  PMID: 4761331

Abstract

The permeability of Sertoli cell tight junctions to lanthanum administered during fixation has been compared in rats after ligation of the ductus deferens and after ligation of the ductuli efferentes. In both control and vasoligated testes, lanthanum penetrated only short distances into the Sertoli cell tight junctions before stopping abruptly. The tight junction, consisting of numerous pentalaminar fusions of contiguous Sertoli cell membranes, prevented diffusion of lanthanum into the adluminal compartment of the seminiferous epithelium. In rats with ligated ductuli efferentes, lanthanum completely permeated many Sertoli cell tight junctions and occupied intercellular spaces of the adluminal compartment. In spite of their newly acquired permeability to lanthanum, tight junctions retained characteristic ultrastructural features, including numerous membrane fusions. When lanthanum-filled tight junctions were sectioned en face, membrane fusions appeared as pale lines in lakes of electron-opaque tracer. These linearly extensive fasciae occludentes occasionally ended blindly, suggesting that lanthanum may have traversed the junction by diffusing around such incomplete barriers. The increased permeability of Sertoli cell tight junctions after efferent ductule ligation, which caused rapid testicular weight gain followed by atrophy, indicates that tight junctions are sensitive to enforced retention of testicular secretions inside the seminiferous tubules. The apparent normalcy of Sertoli cell tight junctions after vasoligation, which had no effect on testis weight, supports the view that blockage of testicular secretions distal to the epididymis is relatively innocuous.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ansbacher R., Keung-Yeung K., Wurster J. C. Sperm antibodies in vasectomized men. Fertil Steril. 1972 Sep;23(9):640–643. [PubMed] [Google Scholar]
  2. Ansbacher R. Sperm-agglutinating and sperm-immobilizing antibodies in vasectomized men. Fertil Steril. 1971 Oct;22(10):629–632. [PubMed] [Google Scholar]
  3. Brightman M. W., Reese T. S. Junctions between intimately apposed cell membranes in the vertebrate brain. J Cell Biol. 1969 Mar;40(3):648–677. doi: 10.1083/jcb.40.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dym M., Fawcett D. W. The blood-testis barrier in the rat and the physiological compartmentation of the seminiferous epithelium. Biol Reprod. 1970 Dec;3(3):308–326. doi: 10.1093/biolreprod/3.3.308. [DOI] [PubMed] [Google Scholar]
  5. FARQUHAR M. G., PALADE G. E. Junctional complexes in various epithelia. J Cell Biol. 1963 May;17:375–412. doi: 10.1083/jcb.17.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fawcett D. W., Leak L. V., Heidger P. M., Jr Electron microscopic observations on the structural components of the blood-testis barrier. J Reprod Fertil Suppl. 1970;10:105–122. [PubMed] [Google Scholar]
  7. Flickinger C. J. Ultrastructure of the rat testis after vasectomy. Anat Rec. 1972 Dec;174(4):477–493. doi: 10.1002/ar.1091740407. [DOI] [PubMed] [Google Scholar]
  8. Friend D. S., Gilula N. B. Variations in tight and gap junctions in mammalian tissues. J Cell Biol. 1972 Jun;53(3):758–776. doi: 10.1083/jcb.53.3.758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goodenough D. A., Revel J. P. A fine structural analysis of intercellular junctions in the mouse liver. J Cell Biol. 1970 May;45(2):272–290. doi: 10.1083/jcb.45.2.272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. JHAVER P. S., OHRI B. B. The history of experimental and clinical work on vasectomy. J Int Coll Surg. 1960 Apr;33:482–486. [PubMed] [Google Scholar]
  11. Johnson M. H. An immunological barrier in the guinea-pig testis. J Pathol. 1970 Jun;101(2):129–139. doi: 10.1002/path.1711010208. [DOI] [PubMed] [Google Scholar]
  12. Johnson M. H. Changes in the blood-testis barrier of the guinea-pig in relation to histological damage following iso-immunization with testis. J Reprod Fertil. 1970 Jun;22(1):119–127. doi: 10.1530/jrf.0.0220119. [DOI] [PubMed] [Google Scholar]
  13. Johnson M. H. Selective damage to spermatogenic cells of high antigenicity during auto-allergic aspermatogenesis. J Pathol. 1970 Nov;102(3):131–138. doi: 10.1002/path.1711020303. [DOI] [PubMed] [Google Scholar]
  14. Johnson M. H. The distribution of immunoglobulin and spermatozoal autoantigen in the genital tract of the male guinea pig: its relationship to autoallergic orchitis. Fertil Steril. 1972 Jun;23(6):383–392. doi: 10.1016/s0015-0282(16)39003-3. [DOI] [PubMed] [Google Scholar]
  15. Kar A. B., Chandra H., Kamboj V. P. Long-term effect of vasectomy on the gonad-pituitary system of rats. Acta Biol Med Ger. 1965;15(4):381–385. [PubMed] [Google Scholar]
  16. Kubota R. [Electron microscopic studies of the testis after vasectomy in rats and men]. Nihon Hinyokika Gakkai Zasshi. 1969 May;60(5):373–397. doi: 10.5980/jpnjurol1928.60.5_373. [DOI] [PubMed] [Google Scholar]
  17. Laumas E. R., Uniyal J. P. Disintegration of spermatozoa & testicular atrophy by a Silastic block of vas deferens in rats. Indian J Exp Biol. 1967 Oct;5(4):199–202. [PubMed] [Google Scholar]
  18. Machen T. E., Erlij D., Wooding F. B. Permeable junctional complexes. The movement of lanthanum across rabbit gallbladder and intestine. J Cell Biol. 1972 Aug;54(2):302–312. doi: 10.1083/jcb.54.2.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Revel J. P., Karnovsky M. J. Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J Cell Biol. 1967 Jun;33(3):C7–C12. doi: 10.1083/jcb.33.3.c7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rümke P., Titus M. Spermagglutinin formation in male rats by subcutaneously injected syngeneic epididymal spermatozoa and by vasoligation or vasectomy. J Reprod Fertil. 1970 Feb;21(1):69–79. doi: 10.1530/jrf.0.0210069. [DOI] [PubMed] [Google Scholar]
  21. Sackler A. M., Weltman A. S., Pandhi V., Schwartz R. Gonadal effects of vasectomy and vasoligation. Science. 1973 Jan 19;179(4070):293–295. doi: 10.1126/science.179.4070.293. [DOI] [PubMed] [Google Scholar]
  22. Schatzki P. F. Bile canaliculus and space of Disse. Electron microscopic relationships as delineated by lanthanum. Lab Invest. 1969 Jan;20(1):87–93. [PubMed] [Google Scholar]
  23. Schatzki P. F. The passage of radioactive lanthanum from the biliary to the vascular system. An electron microscopic and radioactive tracer study. Z Zellforsch Mikrosk Anat. 1971;119(4):451–459. doi: 10.1007/BF00455242. [DOI] [PubMed] [Google Scholar]
  24. Segal R. J. Effect of vasoligation of 14-day-old and 21-day-old rats on spermatogenesis. Int J Fertil. 1972;17(1):33–34. [PubMed] [Google Scholar]
  25. Shea S. M. Lanthanum staining of the surface coat of cells. Its enhancement by the use of fixatives containing Alcian blue or cetylpyridinium chloride. J Cell Biol. 1971 Dec;51(3):611–620. doi: 10.1083/jcb.51.3.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shulman S., Zappi E., Ahmed U., Davis J. E. Immunologic consequences of vasectomy. Contraception. 1972 Apr;5(4):269–278. doi: 10.1016/0010-7824(72)90066-2. [DOI] [PubMed] [Google Scholar]

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