Skip to main content
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1973 Dec 1;59(3):766–771. doi: 10.1083/jcb.59.3.766

RAPID, SIMULTANEOUS MEASUREMENT OF DNA, PROTEIN, AND CELL VOLUME IN SINGLE CELLS FROM LARGE MAMMALIAN CELL POPULATIONS

Harry A Crissman 1, John A Steinkamp 1
PMCID: PMC2109119  PMID: 4128323

Full Text

The Full Text of this article is available as a PDF (373.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams L. R., Kamentsky L. A. Machine characterization of human leukocytes by acridine orange fluorescence. Acta Cytol. 1971 May-Jun;15(3):289–291. [PubMed] [Google Scholar]
  2. Anderson E. C., Bell G. I., Petersen D. F., Tobey R. A. Cell growth and division. IV. Determination of volume growth rate and division probability. Biophys J. 1969 Feb;9(2):246–263. doi: 10.1016/S0006-3495(69)86383-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blomquist C. H., Gregg C. T., Tobey R. A. Enzyme and coenzyme levels, oxygen uptake and lactate production in synchronized cultures of Chinese hamster cells. Exp Cell Res. 1971 May;66(1):75–80. doi: 10.1016/s0014-4827(71)80013-7. [DOI] [PubMed] [Google Scholar]
  4. Bonner W. A., Hulett H. R., Sweet R. G., Herzenberg L. A. Fluorescence activated cell sorting. Rev Sci Instrum. 1972 Mar;43(3):404–409. doi: 10.1063/1.1685647. [DOI] [PubMed] [Google Scholar]
  5. Dittrich W., Göhde W. Impulsfluorometrie bei Einzelezellen in Suspensionen. Z Naturforsch B. 1969 Mar;24(3):360–361. [PubMed] [Google Scholar]
  6. Grover N. B., Naaman J., Ben-Sasson S., Doljanski F. Electrical sizing of particles in suspensions. I. Theory. Biophys J. 1969 Nov;9(11):1398–1414. doi: 10.1016/S0006-3495(69)86461-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holm D. M., Cram L. S. An improved flow microfluorometer for rapid measurement of cell fluorescence. Exp Cell Res. 1973 Jul;80(1):105–110. doi: 10.1016/0014-4827(73)90280-2. [DOI] [PubMed] [Google Scholar]
  8. Julius M. H., Masuda T., Herzenberg L. A. Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence-activated cell sorter. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1934–1938. doi: 10.1073/pnas.69.7.1934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kamentsky L. A., Melamed M. R., Derman H. Spectrophotometer: new instrument for ultrarapid cell analysis. Science. 1965 Oct 29;150(3696):630–631. doi: 10.1126/science.150.3696.630. [DOI] [PubMed] [Google Scholar]
  10. Kraemer P. M., Petersen D. F., Van Dilla M. A. DNA constancy in heteroploidy and the stem line theory of tumors. Science. 1971 Nov 12;174(4010):714–717. doi: 10.1126/science.174.4010.714. [DOI] [PubMed] [Google Scholar]
  11. Kraemer P. M., Tobey R. A., Van Dilla M. A. Flow microfluorometric studies of lectin binding to mammalian cells. I. General features. J Cell Physiol. 1973 Jun;81(3):305–314. doi: 10.1002/jcp.1040810303. [DOI] [PubMed] [Google Scholar]
  12. LePecq J. B., Paoletti C. A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. J Mol Biol. 1967 Jul 14;27(1):87–106. doi: 10.1016/0022-2836(67)90353-1. [DOI] [PubMed] [Google Scholar]
  13. Painter P. R., Marr A. G. Mathematics of microbial populations. Annu Rev Microbiol. 1968;22:519–548. doi: 10.1146/annurev.mi.22.100168.002511. [DOI] [PubMed] [Google Scholar]
  14. Tobey R. A., Crissman H. A., Kraemer P. M. A method for comparing effects of different synchronizing protocols on mammalian cell cycle traverse. The traverse perturbation index. J Cell Biol. 1972 Sep;54(3):638–645. doi: 10.1083/jcb.54.3.638. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tobey R. A., Crissman H. A. Use of flow microfluorometry in detailed analysis of effects of chemical agents on cell cycle progression. Cancer Res. 1972 Dec;32(12):2726–2732. [PubMed] [Google Scholar]
  16. Van Dilla M. A., Trujillo T. T., Mullaney P. F., Coulter J. R. Cell microfluorometry: a method for rapid fluorescence measurement. Science. 1969 Mar 14;163(3872):1213–1214. doi: 10.1126/science.163.3872.1213. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES