Abstract
A specific, 0.1–0.3-µm large ribonucleoprotein complex consisting of a central core with stalklike extensions on top of which 280–320-Å ribonucleoprotein particles are situated is found in an experimentally activated chromosome region, 2–48C, of the polytene chromosomes of Drosophila hydei. Alkaline hydrolysis, RNAse digestion, and uranyl-EDTA-lead staining indicated the ribonucleoprotein character of the 280–320-Å particles, whereas the central core seems to be devoid of RNA. The characteristic complexes are present in the nucleoplasm and at the nuclear membrane, but absent from the cytoplasm. It is suggested that the large RNP complexes are the specific products of the puff at 2–48C. Complexes similar to the ones described have not been observed in any other region of the polytene salivary gland chromosomes of this species.
Full Text
The Full Text of this article is available as a PDF (885.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BEERMANN W., BAHR G. F. The submicroscopic structure of Balbiani-ring. Exp Cell Res. 1954 Feb;6(1):195–201. doi: 10.1016/0014-4827(54)90161-5. [DOI] [PubMed] [Google Scholar]
- Berendes H. D. Factors involved in the expression of gene activity in polytene chromosomes. Chromosoma. 1968;24(4):418–437. doi: 10.1007/BF00285017. [DOI] [PubMed] [Google Scholar]
- Berendes H. D., Meyer G. F. A specific chromosome element, the telomere of Drosophila polytene chromosomes. Chromosoma. 1968;25(2):184–197. doi: 10.1007/BF00327177. [DOI] [PubMed] [Google Scholar]
- Bernhard W. A new staining procedure for electron microscopical cytology. J Ultrastruct Res. 1969 May;27(3):250–265. doi: 10.1016/s0022-5320(69)80016-x. [DOI] [PubMed] [Google Scholar]
- Holt T. K. Local protein accumulation during gene activation. II. Interferometric measurements of the amount of solid material in temperature induced puffs of Drosophila hydei. Chromosoma. 1971 Mar 16;32(4):428–435. doi: 10.1007/BF00285253. [DOI] [PubMed] [Google Scholar]
- Leenders H. J., Berendes H. D. The effect of changes in the respiratory metabolism upon genome activity in Drosophila. I. The induction of gene activity. Chromosoma. 1972;37(4):433–444. doi: 10.1007/BF00284892. [DOI] [PubMed] [Google Scholar]
- Lukanidin E. M., Zalmanzon E. S., Komaromi L., Samarina O. P., Georgiev G. P. Structure and function of informofers. Nat New Biol. 1972 Aug 16;238(85):193–197. doi: 10.1038/newbio238193a0. [DOI] [PubMed] [Google Scholar]
- Monneron A., Bernhard W. Fine structural organization of the interphase nucleus in some mammalian cells. J Ultrastruct Res. 1969 May;27(3):266–288. doi: 10.1016/s0022-5320(69)80017-1. [DOI] [PubMed] [Google Scholar]
- SWIFT H., ADAMS B. J., LARSEN K. ELECTRON MICROSCOPE CYTOCHEMISTRY OF NUCLEIC ACIDS IN DROSOPHILA SALIVARY GLANDS AND TETRAHYMENA. J R Microsc Soc. 1964 Jun;83:161–167. doi: 10.1111/j.1365-2818.1964.tb00525.x. [DOI] [PubMed] [Google Scholar]
- VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]