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. 1974 Jan 1;60(1):26–38. doi: 10.1083/jcb.60.1.26

CHEMICAL CHARACTERIZATION OF THE ISOLATED CELL SURFACE OF AMOEBA

Howard J Allen 1, Charles Ault 1, Richard J Winzler 1, James F Danielli 1
PMCID: PMC2109129  PMID: 4588271

Abstract

The cell surface has been isolated from uninucleate, freshwater, phagocytic amoebae by a new procedure. Several criteria were employed to demonstrate purity of the cell surface fraction. All morphological components of the tripartite surface were present in the isolated surface and the weight of the isolated surface was quantitatively accounted for by the components analyzed. Chemical analyses showed the presence of lipid, protein, and carbohydrate. Mannose was the predominant neutral sugar. Analyses for three different strains of Amoeba were similar. Phosphate was found to be the major anionic group in the cell surface material. Sulfate, uronic acid, sialic acid, muramic acid, and nonamidated glutamic acid and aspartic acid were absent. Evidence is presented suggesting that the phosphate is associated with an unidentified nonreducing polyol.

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Selected References

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  1. Alonso G. L., Tumilasci O. R., Nikonov J. M. Improvement of a direct colorimetric method for calcium determination. Clin Chim Acta. 1970 Mar;27(3):549–551. doi: 10.1016/0009-8981(70)90310-4. [DOI] [PubMed] [Google Scholar]
  2. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  3. BOAS N. F. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
  4. BRANDT P. W., PAPPAS G. D. An electron microscopic study of pinocytosis in ameba. II. The cytoplasmic uptake phase. J Cell Biol. 1962 Oct;15:55–71. doi: 10.1083/jcb.15.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brewer J. E., Bell L. G. Pseudopodium induction by the action of quaternary ammonium ions on Amoeba proteus. J Cell Sci. 1969 Jan;4(1):17–24. doi: 10.1242/jcs.4.1.17. [DOI] [PubMed] [Google Scholar]
  6. Bruce D. L., Marshall J. M., Jr Some ionic and bioelectric properties of the ameba Chaos chaos. J Gen Physiol. 1965 Sep;49(1):151–178. doi: 10.1085/jgp.49.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. CHAPMAN-ANDRESEN C., HOLTER H. Studies on the ingestion of 14C glucose by pinocytosis in the amoeba Chaos chaos. Exp Cell Res. 1955;(Suppl 3):52–63. [PubMed] [Google Scholar]
  8. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  9. Hendil K. B. Ion exchange properties of the glycocalyx of the amoeba Chaos chaos and its relation to pinocytosis. C R Trav Lab Carlsberg. 1971;38(12):187–211. [PubMed] [Google Scholar]
  10. Josefsson J. O. Induction and inhibition of pinocytosis in Amoeba proteus. Acta Physiol Scand. 1968 Aug;73(4):481–490. doi: 10.1111/j.1365-201x.1968.tb10887.x. [DOI] [PubMed] [Google Scholar]
  11. Korn E. D., Olivecrona T. Composition of an amoeba plasma membrane. Biochem Biophys Res Commun. 1971 Oct 1;45(1):90–97. doi: 10.1016/0006-291x(71)90054-4. [DOI] [PubMed] [Google Scholar]
  12. Korn E. D., Wright P. L. Macromolecular composition of an amoeba plasma membrane. J Biol Chem. 1973 Jan 25;248(2):439–447. [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Lehnhardt W. F., Winzler R. J. Determination of neutral sugars in glycoproteins by gas-liquid chromatography. J Chromatogr. 1968 May 7;34(4):471–479. doi: 10.1016/0021-9673(68)80091-3. [DOI] [PubMed] [Google Scholar]
  15. Lorch I. J., Jeon K. W. Character changes induced by heterologous nuclei in amoeba heterokary- ons. Exp Cell Res. 1969 Oct;57(2):223–229. doi: 10.1016/0014-4827(69)90144-x. [DOI] [PubMed] [Google Scholar]
  16. MARSHALL J. M., NACHMIAS V. T. CELL SURFACE AND PINOCYTOSIS. J Histochem Cytochem. 1965 Feb;13:92–104. doi: 10.1177/13.2.92. [DOI] [PubMed] [Google Scholar]
  17. Nachmias V. T. A study by electron microscopy of the formation of new surface by Chaos chaos. Exp Cell Res. 1966 Oct;43(3):583–601. doi: 10.1016/0014-4827(66)90030-9. [DOI] [PubMed] [Google Scholar]
  18. O'NEILL C. H. ISOLATION AND PROPERTIES OF THE CELL SURFACE MEMBRANE OF AMOEBA PROTEUS. Exp Cell Res. 1964 Sep;35:477–496. doi: 10.1016/0014-4827(64)90137-5. [DOI] [PubMed] [Google Scholar]
  19. PENNINGTON R. J. Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase. Biochem J. 1961 Sep;80:649–654. doi: 10.1042/bj0800649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PRESCOTT D. M., JAMES T. W. Culturing of Amoeba proteus on Tetrahymena. Exp Cell Res. 1955 Feb;8(1):256–258. doi: 10.1016/0014-4827(55)90067-7. [DOI] [PubMed] [Google Scholar]
  21. RUSTAD R. C. Pinocytosis. Sci Am. 1961 Apr;204:121–130. [PubMed] [Google Scholar]
  22. Stockem W. Pinocytose und Bewegung von Amöben. 3. Die Funktsion des Golgiapparates von Amoeba proteus und Chaos chaos. Histochemie. 1969;18(3):217–240. doi: 10.1007/BF00306169. [DOI] [PubMed] [Google Scholar]
  23. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]

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