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. 1974 Jan 1;60(1):153–167. doi: 10.1083/jcb.60.1.153

MICROTUBULE SURFACE LATTICE AND SUBUNIT STRUCTURE AND OBSERVATIONS ON REASSEMBLY

Harold P Erickson 1
PMCID: PMC2109150  PMID: 4855592

Abstract

Neuronal microtubules have been reassembled from brain tissue homogenates and purified. In reassembly from purified preparations, one of the first structures formed was a flat sheet, consisting of up to 13 longitudinal filaments, which was identified as an incomplete microtubule wall. Electron micrographs of these flat sheets and intact microtubules were analyzed by optical diffraction, and the surface lattice on which the subunits are arranged was determined to be a 13 filament, 3-start helix. A similar, and probably identical, lattice was found for outer-doublet microtubules. Finally, a 2-D image of the structure and arrangement of the microtubule subunits was obtained by processing selected images with a computer filtering and averaging system. The 40 x 50 Å morphological subunit, which has previously been seen only as a globular particle and identified as the 55,000-dalton tubulin monomer, is seen in this higher resolution reconstructed image to be elongated, and split symmetrically by a longitudinal cleft into two lobes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Behnke O. Incomplete microtubules observed in mammalian blood platelets during microtubule polymerization. J Cell Biol. 1967 Aug;34(2):697–701. doi: 10.1083/jcb.34.2.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Behnke O., Zelander T. Filamentous substructure of microtubules of the marginal bundle of mammalian blood platelets. J Ultrastruct Res. 1967 Jul;19(1):147–165. doi: 10.1016/s0022-5320(67)80065-0. [DOI] [PubMed] [Google Scholar]
  3. Borisy G. G., Olmsted J. B. Nucleated assembly of microtubules in porcine brain extracts. Science. 1972 Sep 29;177(4055):1196–1197. doi: 10.1126/science.177.4055.1196. [DOI] [PubMed] [Google Scholar]
  4. Bryan J., Wilson L. Are cytoplasmic microtubules heteropolymers? Proc Natl Acad Sci U S A. 1971 Aug;68(8):1762–1766. doi: 10.1073/pnas.68.8.1762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chasey D. Subunit arrangement in ciliary microtubules from Tetrahymena pyriformis. Exp Cell Res. 1972 Sep;74(1):140–146. doi: 10.1016/0014-4827(72)90489-2. [DOI] [PubMed] [Google Scholar]
  6. Cohen C., Harrison S. C., Stephens R. E. X-ray diffraction from microtubules. J Mol Biol. 1971 Jul 28;59(2):375–380. doi: 10.1016/0022-2836(71)90057-x. [DOI] [PubMed] [Google Scholar]
  7. DeRosier D. J., Klug A. Structure of the tubular variants of the head of bacteriophage T4 (polyheads). I. Arrangement of subunits in some classes of polyheads. J Mol Biol. 1972 Apr 14;65(3):469–488. doi: 10.1016/0022-2836(72)90202-1. [DOI] [PubMed] [Google Scholar]
  8. DeRosier D. J., Moore P. B. Reconstruction of three-dimensional images from electron micrographs of structures with helical symmetry. J Mol Biol. 1970 Sep 14;52(2):355–369. doi: 10.1016/0022-2836(70)90036-7. [DOI] [PubMed] [Google Scholar]
  9. Grimstone A. V., Klug A. Observations on the substructure of flagellar fibres. J Cell Sci. 1966 Sep;1(3):351–362. doi: 10.1242/jcs.1.3.351. [DOI] [PubMed] [Google Scholar]
  10. Longley W. The crystal structure of bovine liver catalase: a combined study by x-ray diffraction and electron microscopy. J Mol Biol. 1967 Dec 14;30(2):323–327. doi: 10.1016/s0022-2836(67)80042-1. [DOI] [PubMed] [Google Scholar]
  11. Moody M. F. Structure of the sheath of bacteriophage T4. I. Structure of the contracted sheath and polysheath. J Mol Biol. 1967 Apr 28;25(2):167–200. doi: 10.1016/0022-2836(67)90136-2. [DOI] [PubMed] [Google Scholar]
  12. Olmsted J. B., Witman G. B., Carlson K., Rosenbaum J. L. Comparison of the microtubule proteins of neuroblastoma cells, brain, and Chlamydomonas flagella. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2273–2277. doi: 10.1073/pnas.68.9.2273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shelanski M. L., Gaskin F., Cantor C. R. Microtubule assembly in the absence of added nucleotides. Proc Natl Acad Sci U S A. 1973 Mar;70(3):765–768. doi: 10.1073/pnas.70.3.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shelanski M. L., Taylor E. W. Properties of the protein subunit of central-pair and outer-doublet microtubules of sea urchin flagella. J Cell Biol. 1968 Aug;38(2):304–315. doi: 10.1083/jcb.38.2.304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stephens R. E. Thermal fractionation of outer fiber doublet microtubules into A- and B-subfiber components. A- and B-tubulin. J Mol Biol. 1970 Feb 14;47(3):353–363. doi: 10.1016/0022-2836(70)90307-4. [DOI] [PubMed] [Google Scholar]
  16. Warner F. D., Satir P. The substructure of ciliary microtubules. J Cell Sci. 1973 Jan;12(1):313–326. doi: 10.1242/jcs.12.1.313. [DOI] [PubMed] [Google Scholar]
  17. Witman G. B., Carlson K., Rosenbaum J. L. Chlamydomonas flagella. II. The distribution of tubulins 1 and 2 in the outer doublet microtubules. J Cell Biol. 1972 Sep;54(3):540–555. doi: 10.1083/jcb.54.3.540. [DOI] [PMC free article] [PubMed] [Google Scholar]

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