Abstract
The surface morphology of Chinese hamster ovary cells treated with cytochalasin B (CB) has been examined using the scanning electron microscope. The cells respond to treatment with CB by retracting peripheral processes, rounding up, and assuming a smooth or gently convoluted surface. This response occurs within minutes. Cells in different stages of the cell cycle all respond in a similar manner. When CB is removed from treated cells by washing with conditioned medium, the cells regain their normal surface conformation within minutes. The surface topography of these released cells is characteristic of their stage in the cell cycle. Because CB causes an alteration in the morphology of the cell surface and because of the speed of the response and recovery, it is proposed that the primary site of action of CB is the cell surface.
Full Text
The Full Text of this article is available as a PDF (513.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Everhart L. P. Effects of deprivation of two essential amino acids on DNA synthesis in Chinese hamster cells. Exp Cell Res. 1972 Oct;74(2):311–318. doi: 10.1016/0014-4827(72)90382-5. [DOI] [PubMed] [Google Scholar]
- Everhart L. P., Jr, Rubin R. W. Cyclic changes in the cell surface. I. Change in thymidine transport and its inhibition by cytochalasin B in Chinese hamster ovary cells. J Cell Biol. 1974 Feb;60(2):434–441. doi: 10.1083/jcb.60.2.434. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldman R. D., Pollack R., Hopkins N. H. Preservation of normal behavior by enucleated cells in culture. Proc Natl Acad Sci U S A. 1973 Mar;70(3):750–754. doi: 10.1073/pnas.70.3.750. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldman R. D. The effects of cytochalasin B on the microfilaments of baby hamster kidney (BHK-21) cells. J Cell Biol. 1972 Feb;52(2):246–254. doi: 10.1083/jcb.52.2.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Porter K., Prescott D., Frye J. Changes in surface morphology of Chinese hamster ovary cells during the cell cycle. J Cell Biol. 1973 Jun;57(3):815–836. doi: 10.1083/jcb.57.3.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubin R. W., Everhart L. P. The effect of cell-to-cell contact on the surface morphology of Chinese hamster ovary cells. J Cell Biol. 1973 Jun;57(3):837–844. doi: 10.1083/jcb.57.3.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanger J. W., Holtzer H. Cytochalasin B: effects on cell morphology, cell adhesion, and mucopolysaccharide synthesis (cultured cells-contractile microfilaments-glycoproteins-embryonic cells-sorting-out). Proc Natl Acad Sci U S A. 1972 Jan;69(1):253–257. doi: 10.1073/pnas.69.1.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spooner B. S., Yamada K. M., Wessells N. K. Microfilaments and cell locomotion. J Cell Biol. 1971 Jun;49(3):595–613. doi: 10.1083/jcb.49.3.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tobey R. A., Anderson E. C., Petersen D. F. Properties of mitotic cells prepared by mechanically shaking monolayer cultures of Chinese hamster cells. J Cell Physiol. 1967 Aug;70(1):63–68. doi: 10.1002/jcp.1040700109. [DOI] [PubMed] [Google Scholar]
- Tobey R. A., Ley K. D. Isoleucine-mediated regulation of genome repliction in various mammalian cell lines. Cancer Res. 1971 Jan;31(1):46–51. [PubMed] [Google Scholar]
- Wessells N. K., Spooner B. S., Ash J. F., Bradley M. O., Luduena M. A., Taylor E. L., Wrenn J. T., Yamada K. Microfilaments in cellular and developmental processes. Science. 1971 Jan 15;171(3967):135–143. doi: 10.1126/science.171.3967.135. [DOI] [PubMed] [Google Scholar]
- Zigmond S. H., Hirsch J. G. Cytochalasin B: inhibition of D-2-deoxyglucose transport into leukocytes and fibroblasts. Science. 1972 Jun 30;176(4042):1432–1434. doi: 10.1126/science.176.4042.1432. [DOI] [PubMed] [Google Scholar]