Skip to main content
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1974 Feb 1;60(2):507–519. doi: 10.1083/jcb.60.2.507

AN IMPROVED METHOD FOR ISOLATING ALPHA GRANULES AND MITOCHONDRIA FROM HUMAN PLATELETS

M Johan Broekman 1, Nelson P Westmoreland 1, Phin Cohen 1
PMCID: PMC2109166  PMID: 4360293

Full Text

The Full Text of this article is available as a PDF (1.3 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEAUFAY H., BENDALL D. S., BAUDHUIN P., DE DUVE C. Tissue fractionation studies. 12. Intracellular distribution of some dehydrogenases, alkaline deoxyribonuclease and iron in rat-liver tissue. Biochem J. 1959 Dec;73:623–628. doi: 10.1042/bj0730623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BENDALL D. S., DE DUVE C. Tissue-fractionation studies. 14. The activation of latent dehydrogenases in mitochondria from rat liver. Biochem J. 1960 Mar;74:444–450. doi: 10.1042/bj0740444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baggiolini M., Hirsch J. G., De Duve C. Further biochemical and morphological studies of granule fractions from rabbit heterophil leukocytes. J Cell Biol. 1970 Jun;45(3):586–597. doi: 10.1083/jcb.45.3.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barber A. J., Jamieson G. A. Isolation and characterization of plasma membranes from human blood platelets. J Biol Chem. 1970 Dec 10;245(23):6357–6365. [PubMed] [Google Scholar]
  5. Barber A. J., Pepper D. S., Jamieson G. A. A comparison of methods for platelet lysis and the isolation of platelet membranes. Thromb Diath Haemorrh. 1971 Aug 31;26(1):38–57. [PubMed] [Google Scholar]
  6. Brunk U. T., Ericsson J. L. Cytochemical evidence for the leakage of acid phosphatase through ultrastructurally intact lysosomal membranes. Histochem J. 1972 Nov;4(6):479–491. doi: 10.1007/BF01011128. [DOI] [PubMed] [Google Scholar]
  7. COOPERSTEIN S. J., LAZAROW A., KURFESS N. J. A microspectrophotometric method for the determination of succinic dehydrogenase. J Biol Chem. 1950 Sep;186(1):129–139. [PubMed] [Google Scholar]
  8. CRANE R. K. The substrate specificity of liver glucose-6-phosphatase. Biochim Biophys Acta. 1955 Jul;17(3):443–444. doi: 10.1016/0006-3002(55)90396-6. [DOI] [PubMed] [Google Scholar]
  9. DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. DE DUVE C., WATTIAUX R. Tissue fractionation studies. VII. Release of bound hydrolases by means of triton X-100. Biochem J. 1956 Aug;63(4):606–608. doi: 10.1042/bj0630606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Day H. J., Holmsen H., Hovig T. Subcellular particles of human platelets. A biochemical and electron microscopic study with particular reference to the influence of fractionation techniques. Scand J Haematol Suppl. 1969;7:3–35. [PubMed] [Google Scholar]
  12. Day H. J., Stormorken H., Holmsen H. Subcellular localization of platelet factor 3 and platelet factor 4. Scand J Haematol. 1973;10(4):254–260. doi: 10.1111/j.1600-0609.1973.tb00069.x. [DOI] [PubMed] [Google Scholar]
  13. Demers L. M., Gabbe S. G., Villee C. A., Greep R. O. The effects of insulin on human placental glycogenesis. Endocrinology. 1972 Jul;91(1):270–275. doi: 10.1210/endo-91-1-270. [DOI] [PubMed] [Google Scholar]
  14. Derksen A., Cohen P. Extensive incorporation of (2-14C) mevalonic acid into cholesterol precursors by human platelets in vitro. J Biol Chem. 1973 Nov 10;248(21):7396–7403. [PubMed] [Google Scholar]
  15. GIANETTO R., DE DUVE C. Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, beta-glucuronidase and cathepsin by rat-liver particles. Biochem J. 1955 Mar;59(3):433–438. doi: 10.1042/bj0590433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. HOGEBOOM G. H., SCHNEIDER W. C. Intracellular distribution of enzymes. XI. Glutamic dehydrogenase. J Biol Chem. 1953 Sep;204(1):233–238. [PubMed] [Google Scholar]
  17. Harris G. L., Crawford N. Isolation of pig platelet membranes and granules. Distribution and validity of marker enzymes. Biochim Biophys Acta. 1973 Feb 16;291(3):701–719. doi: 10.1016/0005-2736(73)90475-6. [DOI] [PubMed] [Google Scholar]
  18. Holmsen H., Day H. J. The selectivity of the thrombin-induced platelet release reaction: subcellular localization of released and retained constituents. J Lab Clin Med. 1970 May;75(5):840–855. [PubMed] [Google Scholar]
  19. Kaulen H. D., Gross R. The differentiation of acid phosphatases of human blood platelets. Thromb Diath Haemorrh. 1971 Oct 31;26(2):353–361. [PubMed] [Google Scholar]
  20. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  21. Marcus A. J., Zucker-Franklin D., Safier L. B., Ullman H. L. Studies on human platelet granules and membranes. J Clin Invest. 1966 Jan;45(1):14–28. doi: 10.1172/JCI105318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Minter B. F., Crawford N. Subcellular distribution of 5 hydroxytryptamine in pig platelets. Biochem Pharmacol. 1971 Apr;20(4):783–802. doi: 10.1016/0006-2952(71)90041-4. [DOI] [PubMed] [Google Scholar]
  23. Schnaitman C., Greenawalt J. W. Enzymatic properties of the inner and outer membranes of rat liver mitochondria. J Cell Biol. 1968 Jul;38(1):158–175. doi: 10.1083/jcb.38.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Siegel A., Burri P. H., Weibel E. R., Bettex-Galland M., Lüscher E. F. Density gradient centrifugation and electron microscopic characterization of subcellular fractions from human blood platelets. Thromb Diath Haemorrh. 1971 Jun 30;25(2):252–267. [PubMed] [Google Scholar]
  25. Siegel A., Lüscher E. F. Non-identity of the alpha-granules of human blood platelets with typical lysosomes. Nature. 1967 Aug 12;215(5102):745–747. doi: 10.1038/215745a0. [DOI] [PubMed] [Google Scholar]
  26. Siekevitz P. [The organization of biologic membranes]. N Engl J Med. 1970 Nov 5;283(19):1035–1041. doi: 10.1056/NEJM197011052831908. [DOI] [PubMed] [Google Scholar]
  27. Sottocasa G. L., Kuylenstierna B., Ernster L., Bergstrand A. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. J Cell Biol. 1967 Feb;32(2):415–438. doi: 10.1083/jcb.32.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Stahn R., Maier K. P., Hannig K. A new method for the preparation of rat liver lysosomes. Separation of cell organelles of rat liver by carrier-free continuous electrophoresis. J Cell Biol. 1970 Sep;46(3):576–591. doi: 10.1083/jcb.46.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Vaes G., Jacques P. Studies on bone enzymes. The assay of acid hydrolases and other enzymes in bone tissue. Biochem J. 1965 Nov;97(2):380–388. doi: 10.1042/bj0970380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. WURTMAN R. J., AXELROD J. A SENSITIVE AND SPECIFIC ASSAY FOR THE ESTIMATION OF MONOAMINE OXIDASE. Biochem Pharmacol. 1963 Dec;12:1439–1441. doi: 10.1016/0006-2952(63)90215-6. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES