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. 1974 Jul 1;62(1):198–214. doi: 10.1083/jcb.62.1.198

THE UPPER CELL SURFACE: ITS INABILITY TO SUPPORT ACTIVE CELL MOVEMENT IN CULTURE

Albert DiPasquale 1, Paul B Bell Jr 1
PMCID: PMC2109182  PMID: 4600884

Abstract

A variety of epithelial cells and fibroblasts fail to move over one another's upper surfaces in culture, resulting in monolayering. The failure of seeded fibroblasts to adhere to and spread on epithelial cell surfaces suggests that monolayering in culture is due to the lack of adhesion of the upper cell surface, at least of epithelial cells. Seeded fibroblasts and postmitotic, rounded fibroblasts likewise fail to spread on the upper surfaces of spread fibroblasts, suggesting that the inability of the upper cell surface to support spreading may be a general phenomenon. Inert particles and cell processes do not adhere directly to the upper cell surface. However, they can initiate adhesions to the surface at a cell's free margin, suggesting a variation of adhesive properties over a cell's surface.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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