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. 1974 Jul 1;62(1):114–131. doi: 10.1083/jcb.62.1.114

KERATIN SYNTHESIS DURING DEVELOPMENT OF THE EMBRYONIC CHICK FEATHER

D J Kemp 1, P Y Dyer 1, G E Rogers 1
PMCID: PMC2109183  PMID: 4407045

Abstract

The synthesis of keratin proteins during development of the embryonic chick feather was studied by quantitative gel electrophoresis of the reduced and carboxymethylated proteins. The results demonstrated a coordinated synthesis of the major keratin proteins, during and after the onset of keratin synthesis. The results from gel electrophoresis correlated well with electron microscope visualization or keratin fibrils in the developing feathers. Autoradiography at the electron microscope level indicated that the feather cells lose the ability to synthesize DNA before keratin synthesis begins, but retain the ability to synthesize RNA after keratin synthesis begins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BELL E. PROTEIN SYNTHESIS IN DIFFERENTIATING CHICK SKIN. Natl Cancer Inst Monogr. 1964 Apr;13:1–11. [PubMed] [Google Scholar]
  2. BELL E., THATHACHARI Y. T. Development of feather keratin during embryogenesis of the chick. J Cell Biol. 1963 Feb;16:215–223. doi: 10.1083/jcb.16.2.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BEN-OR S., BELL E. SKIN ANTIGENS IN THE CHICK EMBRYO IN RELATION TO OTHER DEVELOPMENTAL EVENTS. Dev Biol. 1965 Apr;11:184–201. doi: 10.1016/0012-1606(65)90056-4. [DOI] [PubMed] [Google Scholar]
  4. Chrambach A., Reisfeld R. A., Wyckoff M., Zaccari J. A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis. Anal Biochem. 1967 Jul;20(1):150–154. doi: 10.1016/0003-2697(67)90272-2. [DOI] [PubMed] [Google Scholar]
  5. DOWNES A. M., SHARRY L. F., ROGERS G. E. SEPARATE SYNTHESIS OF FIBRILLAR AND MATRIX PROTEINS IN THE FORMATION OF KERATIN. Nature. 1963 Sep 14;199:1059–1061. doi: 10.1038/1991059a0. [DOI] [PubMed] [Google Scholar]
  6. Dahlberg A. E., Dingman C. W., Peacock A. C. Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels. J Mol Biol. 1969 Apr 14;41(1):139–147. doi: 10.1016/0022-2836(69)90131-4. [DOI] [PubMed] [Google Scholar]
  7. FUKUYAMA K., BERNSTEIN I. A. Autoradiographic studies of the incorporation of thymidine-H3 into deoxyribonucleic acid in the skin of young rats. J Invest Dermatol. 1961 May;36:321–326. doi: 10.1038/jid.1961.53. [DOI] [PubMed] [Google Scholar]
  8. Fraser I. E. Proteins of keratin and their synthesis. I. Proteins of prekeratin and keratin. Aust J Biol Sci. 1969 Feb;22(1):213–229. [PubMed] [Google Scholar]
  9. Fraser I. E. Proteins of keratin and their synthesis. II. Incorporation of [35S]cystine into prekeratin and keratin proteins. Aust J Biol Sci. 1969 Feb;22(1):231–238. [PubMed] [Google Scholar]
  10. Kemp D. J., Rogers G. E. Differentiation of avian keratinocytes. Characterization and relationships of the keratin proteins of adult and embryonic feathers and scales. Biochemistry. 1972 Mar 14;11(6):969–975. doi: 10.1021/bi00756a005. [DOI] [PubMed] [Google Scholar]
  11. Kischer C. W., Furlong N. B. DNA polymerase activity in extracts of the developing chick skin and down feather organ. Proc Soc Exp Biol Med. 1967 Apr;124(4):1188–1190. doi: 10.3181/00379727-124-31960. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Malt R. A., Bell E. Feather proteins during embyronic development. Nature. 1965 Mar 13;205(976):1081–1083. doi: 10.1038/2051081a0. [DOI] [PubMed] [Google Scholar]
  14. O'Donnell I. J. The complete amino acid sequence of a feather keratin from emu (Dromaius novae-hollandiae). Aust J Biol Sci. 1973 Apr;26(2):415–435. doi: 10.1071/bi9730415. [DOI] [PubMed] [Google Scholar]
  15. Smith K. B. The proteins of the embryonic chick epidermis. I. During the normal development in ovo. Dev Biol. 1973 Feb;30(2):249–262. doi: 10.1016/0012-1606(73)90087-0. [DOI] [PubMed] [Google Scholar]
  16. Yatvin M. B. Hypophyseal control of genetic expression during chick feather and skin differentiation. Science. 1966 Jul 8;153(3732):184–185. doi: 10.1126/science.153.3732.184. [DOI] [PubMed] [Google Scholar]

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