Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Mar;170(3):1380–1383. doi: 10.1128/jb.170.3.1380-1383.1988

Replication patterns of multiple plasmids coexisting in Escherichia coli.

A C Leonard 1, C E Helmstetter 1
PMCID: PMC210920  PMID: 3277954

Abstract

The replication patterns of several plasmids were measured simultaneously during the cell division cycle of Escherichia coli B/r. F plasmids harboring oriS, both oriS and oriV, pSC101, and pBR322 were found to replicate at all stages of the cell division cycle with kinetics which were indistinguishable from one another and clearly different from the periodic synthesis of the minichromosomes pAL49 and pAL70.

Full text

PDF
1380

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andresdottir V., Masters M. Evidence that F' lac replicates asynchronously during the cell cycle of Escherichia coli B/r. Mol Gen Genet. 1978 Jul 11;163(2):205–212. doi: 10.1007/BF00267411. [DOI] [PubMed] [Google Scholar]
  2. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  3. Cohen S. N., Chang A. C. Revised interpretation of the origin of the pSC101 plasmid. J Bacteriol. 1977 Nov;132(2):734–737. doi: 10.1128/jb.132.2.734-737.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collins J., Pritchard R. H. Relationship between chromosome replication and F'lac episome replication in Escherichia coli. J Mol Biol. 1973 Jun 25;78(1):143–155. doi: 10.1016/0022-2836(73)90434-8. [DOI] [PubMed] [Google Scholar]
  5. Cooper S. Relationship of Flac replication and chromosome replication. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2706–2710. doi: 10.1073/pnas.69.9.2706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davis D. B., Helmstetter C. E. Control of F'lac replication in Escherichia coli B-r. J Bacteriol. 1973 Apr;114(1):294–299. doi: 10.1128/jb.114.1.294-299.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Finkelstein M., Helmstetter C. E. Cell cycle analysis of F'lac replication in Escherichia coli B/r. J Bacteriol. 1977 Dec;132(3):884–895. doi: 10.1128/jb.132.3.884-895.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Frey J., Chandler M., Caro L. The effects of an Escherichia coli dnaAts mutation on the replication of the plasmids colE1 pSC101, R100.1 and RTF-TC. Mol Gen Genet. 1979 Jul 13;174(2):117–126. doi: 10.1007/BF00268349. [DOI] [PubMed] [Google Scholar]
  9. Gustafsson P., Nordström K., Perram J. W. Selection and timing of replication of plasmids R1drd-19 and F'lac in Escherichia coli. Plasmid. 1978 Feb;1(2):187–203. doi: 10.1016/0147-619x(78)90038-0. [DOI] [PubMed] [Google Scholar]
  10. Hansen E. B., Yarmolinsky M. B. Host participation in plasmid maintenance: dependence upon dnaA of replicons derived from P1 and F. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4423–4427. doi: 10.1073/pnas.83.12.4423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hasunuma K., Sekiguchi M. Replication of plasmid pSC101 in Escherichia coli K12: requirement for dnaA function. Mol Gen Genet. 1977 Sep 9;154(3):225–230. doi: 10.1007/BF00571277. [DOI] [PubMed] [Google Scholar]
  12. Helmstetter C. E., Leonard A. C. Coordinate initiation of chromosome and minichromosome replication in Escherichia coli. J Bacteriol. 1987 Aug;169(8):3489–3494. doi: 10.1128/jb.169.8.3489-3494.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Helmstetter C. E., Leonard A. C. Mechanism for chromosome and minichromosome segregation in Escherichia coli. J Mol Biol. 1987 Sep 20;197(2):195–204. doi: 10.1016/0022-2836(87)90118-5. [DOI] [PubMed] [Google Scholar]
  14. Hiraga S., Jaffé A., Ogura T., Mori H., Takahashi H. F plasmid ccd mechanism in Escherichia coli. J Bacteriol. 1986 Apr;166(1):100–104. doi: 10.1128/jb.166.1.100-104.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kahn M., Kolter R., Thomas C., Figurski D., Meyer R., Remaut E., Helinski D. R. Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2. Methods Enzymol. 1979;68:268–280. doi: 10.1016/0076-6879(79)68019-9. [DOI] [PubMed] [Google Scholar]
  16. Kline B. C. A review of mini-F plasmid maintenance. Plasmid. 1985 Jul;14(1):1–16. doi: 10.1016/0147-619x(85)90027-7. [DOI] [PubMed] [Google Scholar]
  17. Kline B. C., Kogoma T., Tam J. E., Shields M. S. Requirement of the Escherichia coli dnaA gene product for plasmid F maintenance. J Bacteriol. 1986 Oct;168(1):440–443. doi: 10.1128/jb.168.1.440-443.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Laskey R. A. The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis. Methods Enzymol. 1980;65(1):363–371. doi: 10.1016/s0076-6879(80)65047-2. [DOI] [PubMed] [Google Scholar]
  19. Leonard A. C., Helmstetter C. E. Cell cycle-specific replication of Escherichia coli minichromosomes. Proc Natl Acad Sci U S A. 1986 Jul;83(14):5101–5105. doi: 10.1073/pnas.83.14.5101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lovett M. A., Helinski D. R. Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid. J Bacteriol. 1976 Aug;127(2):982–987. doi: 10.1128/jb.127.2.982-987.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nordström K. Control of plasmid replication: theoretical considerations and practical solutions. Basic Life Sci. 1985;30:189–214. doi: 10.1007/978-1-4613-2447-8_17. [DOI] [PubMed] [Google Scholar]
  22. Oka A., Sugimoto K., Takanami M., Hirota Y. Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication. Mol Gen Genet. 1980 Apr;178(1):9–20. doi: 10.1007/BF00267207. [DOI] [PubMed] [Google Scholar]
  23. Prentki P., Chandler M., Caro L. Replication of prophage P1 during the cell cycle of Escherichia coli. Mol Gen Genet. 1977 Mar 28;152(1):71–76. doi: 10.1007/BF00264942. [DOI] [PubMed] [Google Scholar]
  24. Projan S. J., Carleton S., Novick R. P. Determination of plasmid copy number by fluorescence densitometry. Plasmid. 1983 Mar;9(2):182–190. doi: 10.1016/0147-619x(83)90019-7. [DOI] [PubMed] [Google Scholar]
  25. Scott J. R. Regulation of plasmid replication. Microbiol Rev. 1984 Mar;48(1):1–23. doi: 10.1016/b978-0-12-048850-6.50006-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shields M. S., Kline B. C., Tam J. E. Similarities in control of mini-F plasmid and chromosomal replication in Escherichia coli. J Bacteriol. 1987 Jul;169(7):3375–3378. doi: 10.1128/jb.169.7.3375-3378.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Steinberg D. A., Helmstetter C. E. F plasmid replication and the division cycle of Escherichia coli B/r. Plasmid. 1981 Nov;6(3):342–353. doi: 10.1016/0147-619x(81)90042-1. [DOI] [PubMed] [Google Scholar]
  28. Zeuthen J., Pato M. L. Replication of the F'lac sex factor in the cell cycle of Escherichia coli. Mol Gen Genet. 1971;111(3):242–255. doi: 10.1007/BF00433109. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES