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. 1974 Apr 1;61(1):188–200. doi: 10.1083/jcb.61.1.188

ANALYTICAL STUDY OF MICROSOMES AND ISOLATED SUBCELLULAR MEMBRANES FROM RAT LIVER

I. Biochemical Methods

Henri Beaufay 1, Alain Amar-Costesec 1, Ernest Feytmans 1, Denise Thinès-Sempoux 1, Maurice Wibo 1, Mariette Robbi 1, Jacques Berthet 1
PMCID: PMC2109252  PMID: 4150488

Abstract

The series introduced by this paper reports the results of a detailed analysis of the microsomal fraction from rat liver by density gradient centrifugation. The biochemical methods used throughout this work for the determination of monoamine oxidase, NADH cytochrome c reductase, NADPH cytochrome c reductase, cytochrome oxidase, catalase, aminopyrine demethylase, cytochromes b 5 and P 450, glucuronyltransferase, galactosyltransferase, esterase, alkaline and acid phosphatases, 5'-nucleotidase, glucose 6-phosphatase, alkaline phosphodiesterase I, N-acetyl-β-glucosaminidase, β-glucuronidase, nucleoside diphosphatase, aldolase, fumarase, glutamine synthetase, protein, phospholipid, cholesterol, and RNA are described and justified when necessary.

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Selected References

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