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. 1974 Apr 1;61(1):156–165. doi: 10.1083/jcb.61.1.156

ASSOCIATION OF CALCIUM WITH MEMBRANES OF SQUID GIANT AXON

Ultrastructure and Microprobe Analysis

J L Oschman 1, T A Hall 1, P D Peters 1, B J Wall 1
PMCID: PMC2109275  PMID: 4132064

Abstract

Giant axons from the squid, Loligo pealei, were fixed in glutaraldehyde and postfixed in osmium tetroxide. Calcium chloride (5 mM/liter) was added to all aqueous solutions used for tissue processing. Electron-opaque deposits were found along the axonal plasma membranes, within mitochondria, and along the basal plasma membranes of Schwann cells. X-ray microprobe analysis (EMMA-4) yielded signals for calcium and phosphorus when deposits were probed, whereas these elements were not detected in the axoplasm.

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Selected References

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