Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1974 Dec 1;63(3):986–997. doi: 10.1083/jcb.63.3.986

EFFECTS OF CYTOCHALASIN B ON THE RESPONSE OF TOAD URINARY BLADDER TO VASOPRESSIN

Walter L Davis 1, David B P Goodman 1, Richard J Schuster 1, Howard Rasmussen 1, James H Martin 1
PMCID: PMC2109355  PMID: 4373479

Abstract

A combined physiological and morphological study of the effects of cytochalasin B (CB) on the toad urinary bladder has been carried out. CB inhibits the hydro-osmotic response to vasopressin without altering basal water permeability or diffusion, or the increase in 3H2O diffusion observed after hormone addition. Although CB increases [22Na]-, [36Cl]-, and [14C]urea fluxes, and decreases transepithelial potential, no alteration in basal short-circuit current, the vasopressin-induced increase in this parameter, or [14C]inulin permeability occurs. In the absence of hormone, CB does not markedly alter the structure of the toad bladder. However, in the presence of vasopressin, CB induces the formation of large intracellular vacuoles. These results suggest a possible coupling of solute and water movement across the tissue.

Full Text

The Full Text of this article is available as a PDF (1.2 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENTLEY P. J. The effects of neurohypophysial extracts on the water transfer across the wall of the isolated urinary bladder of the toad Bufo marinus. J Endocrinol. 1958 Sep;17(3):201–209. doi: 10.1677/joe.0.0170201. [DOI] [PubMed] [Google Scholar]
  2. CHOI J. K. The fine structure of the urinary bladder of the toad, Bufo marinus. J Cell Biol. 1963 Jan;16:53–72. doi: 10.1083/jcb.16.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohn R. H., Banerjee S. D., Shelton E. R., Bernfield M. R. Cytochalasin B: lack of effect on mucopolysaccharide synthesis and selective alterations in precursor uptake. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2865–2869. doi: 10.1073/pnas.69.10.2865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diamond J. M. Water-solute coupling and ion selectivity in epithelia. Philos Trans R Soc Lond B Biol Sci. 1971 Aug 20;262(842):141–151. doi: 10.1098/rstb.1971.0085. [DOI] [PubMed] [Google Scholar]
  5. Finn A. L., Rockoff M. L. The kinetics of sodium transport in the toad bladder. I. Determination of the transport pool. J Gen Physiol. 1971 Mar;57(3):326–348. doi: 10.1085/jgp.57.3.326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goodman D. B., Allen J. E., Rasmussen H. On the mechanism of action of aldosterone. Proc Natl Acad Sci U S A. 1969 Sep;64(1):330–337. doi: 10.1073/pnas.64.1.330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grantham J., Cuppage F. E., Fanestil D. Direct observation of toad bladder response to vasopressin. J Cell Biol. 1971 Mar;48(3):695–699. doi: 10.1083/jcb.48.3.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Handler J. S., Butcher R. W., Sutherland E. W., Orloff J. The effect of vasopressin and of theophylline on the concentration of adenosine 3',5'-phosphate in the urinary bladder of the toad. J Biol Chem. 1965 Nov;240(11):4524–4526. [PubMed] [Google Scholar]
  10. Kletzien R. F., Perdue J. F., Springer A. Cytochalasin A and B. Inhibition of sugar uptake in cultured cells. J Biol Chem. 1972 May 10;247(9):2964–2966. [PubMed] [Google Scholar]
  11. LEAF A., HAYS R. M. Permeability of the isolated toad bladder to solutes and its modification by vasopressin. J Gen Physiol. 1962 May;45:921–932. doi: 10.1085/jgp.45.5.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Leaf A. Membrane effects of antidiuretic hormone. Am J Med. 1967 May;42(5):745–756. doi: 10.1016/0002-9343(67)90092-7. [DOI] [PubMed] [Google Scholar]
  14. Levine S., Franki N., Hays R. M. Effect of phloretin on water and solute movement in the toad bladder. J Clin Invest. 1973 Jun;52(6):1435–1442. doi: 10.1172/JCI107317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Masur S. K., Holtzman E., Walter R. Hormone-stimulated exocytosis in the toad urinary bladder. Some possible implications for turnover of surface membranes. J Cell Biol. 1972 Jan;52(1):211–219. doi: 10.1083/jcb.52.1.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mizel S. B., Wilson L. Inhibition of the transport of several hexoses in mammalian cells by cytochalasin B. J Biol Chem. 1972 Jun 25;247(12):4102–4105. [PubMed] [Google Scholar]
  17. ORLOFF J., HANDLER J. S. The similarity of effects of vasopressin, adenosine-3',5'-phosphate (cyclic AMP) and theophylline on the toad bladder. J Clin Invest. 1962 Apr;41:702–709. doi: 10.1172/JCI104528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. PEACHEY L. D., RASMUSSEN H. Structure of the toad's urinary bladder as related to its physiology. J Biophys Biochem Cytol. 1961 Aug;10:529–553. doi: 10.1083/jcb.10.4.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Parisi M., Piccinni Z. F. The penetration of water into the epithelium of toad urinary bladder and its modification by oxytocin. J Membr Biol. 1973;12(3):227–246. doi: 10.1007/BF01870003. [DOI] [PubMed] [Google Scholar]
  20. Plagemann P. G., Estensen R. D. Cytochalasin B. VI. Competitive inhibition of nucleoside transport by cultured Novikoff rat hepatoma cells. J Cell Biol. 1972 Oct;55(1):179–185. doi: 10.1083/jcb.55.1.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sanger J. W., Holtzer H. Cytochalasin B: effects on cell morphology, cell adhesion, and mucopolysaccharide synthesis (cultured cells-contractile microfilaments-glycoproteins-embryonic cells-sorting-out). Proc Natl Acad Sci U S A. 1972 Jan;69(1):253–257. doi: 10.1073/pnas.69.1.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schroeder T. E. The contractile ring. I. Fine structure of dividing mammalian (HeLa) cells and the effects of cytochalasin B. Z Zellforsch Mikrosk Anat. 1970;109(4):431–449. [PubMed] [Google Scholar]
  23. Taylor A., Mamelak M., Reaven E., Maffly R. Vasopressin: possible role of microtubules and microfilaments in its action. Science. 1973 Jul 27;181(4097):347–350. doi: 10.1126/science.181.4097.347. [DOI] [PubMed] [Google Scholar]
  24. Wessells N. K., Spooner B. S., Ash J. F., Bradley M. O., Luduena M. A., Taylor E. L., Wrenn J. T., Yamada K. Microfilaments in cellular and developmental processes. Science. 1971 Jan 15;171(3967):135–143. doi: 10.1126/science.171.3967.135. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES